Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass... I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands) #WesternBlot #cellsky

Persistent hindrances to data re-use in single-cell genomics https://www.biorxiv.org/content/10.1101/2025.10.02.680150v1

My Own Lab at Westlake University is OPEN! We are recruiting cool people at all levels NOW! By tinkering behaviors and neural circuits in distinct animal species (mouse, sugar glider, etc), we are exploring new ways of doing social neuroscience in vivo, in silico and at home!

Every single one of these leaves is a bug.

It's probably the deterioration of social media (I hope) but i'm exposed to a lot less cool science. It used to feel like a new cool study is being preprinted/published every week, and lately it's rare (at least so it seems). Share a recent study that's worth knowing!

mRNA-based sensors are now more modular, but still rely on overexpression of ADAR1 which interferes with endogenous biology. Seems to be a situation where enzyme engineering to make a bio-orthogonal ADAR-like protein that only deaminates the reporter should be within reach.

The great thing about Rosh Hashanah and Fat Bear Week starting the same day is apples and honey is completely appropriate for both

This is truly an incredible breakthrough IMO. Really exemplifies what you get when deep domain expertise (popgen/evolution/disease genetics in this case) fuses with cleverly crafted ML. What u get r sleek, well thought out architectures that absolutely destroy the behemoths. Wow!! 1/

Asking BlueSky for help: For a review, I am trying to accurately credit the first paper that measured pairwise 3D distances between 2 pieces of DNA on the same chromosome (or cosmid). Is Trask 1989 the first? I know of earlier single-locus papers (1982). www.sciencedirect.com/science/arti...

Against my better instincts, I have written some notes on how human probability judgements work and what you should expect from surveys that ask people to guess what proportion of the population is transgender. I hope never to speak of this matter again

Jamie Drayton and I were fortunate to contribute some RCMC analyses to this beautiful paper from Eder, Moene...van Steensel that systematically maps the relationship between enhancer location and gene expression (and nice to see RCMC predict expression in Fig 2H-I): www.science.org/doi/10.1126/...

Very interesting paper from the group of Alistair Boettiger examining DNA contact using long-term live cell imaging. Generated ~75TB of data! www.science.org/doi/10.1126/...

New preprint from our lab! What can we learn about the properties of gene regulatory elements by CRISPR’ing a random set of accessible sites in human cells? Find out here: www.biorxiv.org/content/10.1... 👇 1/

🚨 Request for functional genomics / GWAS folks. I repeatedly see claims that “… 90% of GWAS identified variants lie in regulatory regions…” from reviews, but have had a hard time tracking down where this number comes from. 🧵@sashagusevposts.bsky.social @jkpritch.bsky.social @tuuliel.bsky.social

Next-generation hybridization chain reaction tools with enhanced sensitivities to detect challenging targets https://www.biorxiv.org/content/10.1101/2025.09.09.675218v1

@clemmensenc.bsky.social you have a very nice 🧵 on this great review over at the other site. Would love to have something similar here www.cell.com/cell/fulltex...

Utilizing in silico designed protein binders to inhibit mismatch repair and increase editing efficiency of Cas9-mediated Prime Editing www.cell.com/cell/fulltex...

Important paper comparing TF footprinting methods for single-cell genomics studies from Katherine Pollard’s group at UCSF

How does the structure of a neural circuit shape its function? @neuralreckoning.bsky.social & I explore this in our new preprint: doi.org/10.1101/2025... 🤖🧠🧪 🧵1/9

Great thread and paper describing context-specific eQTL discovery

Your brain 🧠 senses the concentration of ions dissolved in your blood 🩸 and if too high, generates the feeling of “thirst” to induced water💧consumption. New results show that TMEM63B is the molecular sensor in the brain that detects blood osmolarity www.cell.com/neuron/fullt...

🎯Enrich for your favorite cell-type 🧬No transgenic modification neccessary 💰Save costly sequencing for targeted populations

Hey @lgmartelotto.bsky.social, I’m excited about your FixNCut protocol that’s works on tissues up to 3x3x3 mm of mouse tissue. Any idea how this protocol might perform on a whole mouse brain (~8x5x4 mm)?

Terrific piece by Danielle Allen in @theharvardcrimson.bsky.social

On the x-axis is every human gene, ranked by number of publications containing mention of the gene name. Lots left to discover...

Neat use of structured connectivity and restricted sampling to mimic dendrites in an ANN. www.nature.com/articles/s41...

I'm always curious to see what types of 🐭 mouse behavioral assays folks are using. A recent paper examining distinct BLA circuits in anxiety had some good graphics of traditional anxiety assays. 🧵👇 www-cell-com.ezp-prod1.hul.harvard.edu/neuron/fullt...

This is a question that I've asked myself as well, but coming from a more molecular/genetics neuroscience background. Here are some some examples in multiple brain areas that come to mind I hope others find useful...🧵👇