3D multi-omics tumour atlases: from technology to biology and clinical translation www.nature.com/articles/s41...

It's cool to see we aren't the only ones that think rationally designed probes + gapfilling is the future. Check out a corroborating workflow from @mgrillo.bsky.social, @lgmartelotto.bsky.social, Anna Nam, and colleagues: bsky.app/profile/mgri... 7/n

I had the “Filling” this week was going to be great! Brought to you by @danapeer.bsky.social @ronanchaligne.bsky.social and @caleblareau.bsky.social Sydney Blattman = ⭐️ Genotyping in fixed transcriptomes resolves clonal heterogeneity via single-cell sequencing. www.biorxiv.org/content/10.6...

New work from our @mgrillo.bsky.social with @lgmartelotto.bsky.social and others. Capture somatic mutations in single-cell assays via RNA-targeted gap filling. Check it out!

Capturing somatic mutations in single-cell probe-based assays (ie. 10X Flex) is a hard task, especially if you don't know *exactly* the mutations you want to capture, or if your target sequence is a mutational hotspot with many possible alternative alleles. Here's the way we pulled this off. Enjoy!

We GoT a Filling…uuuuhoooo 𝗧𝗮𝗿𝗴𝗲𝘁𝗲𝗱 𝘀𝗲𝗾𝘂𝗲𝗻𝗰𝗶𝗻𝗴 𝗼𝗳 𝗺𝘂𝘁𝗮𝘁𝗶𝗼𝗻𝘀 𝘃𝗶𝗮 𝗥𝗡𝗔-𝘁𝗲𝗺𝗽𝗹𝗮𝘁𝗲𝗱 𝗴𝗮𝗽 𝗳𝗶𝗹𝗹𝗶𝗻𝗴 𝗼𝗳 𝗼𝗹𝗶𝗴𝗼𝗻𝘂𝗰𝗹𝗲𝗼𝘁𝗶𝗱𝗲𝘀 𝗳𝗼𝗿 𝘀𝗶𝗻𝗴𝗹𝗲-𝗰𝗲𝗹𝗹 𝗥𝗡𝗔-𝘀𝗲𝗾 www.biorxiv.org/content/10.6...

Why so serious? 🃏 Marco Grillo, Anna Nam and yours truly 𝗚𝗼𝗧 𝗮 𝗙𝗶𝗹𝗹𝗶𝗻𝗴 that will make you happy 😃 No more limitations. Maximum flexibility. Something is coming. Stay tuned. #singlecellwithaplus

2026 HCA Asia Meeting in the land of Oz! 🫶🫶🫶 events.humancellatlas.org/2026asia/Home

Enjoyed writing this Preview with Jonas Gudera on GoT-Multi 🪄 A powerful #singlecell approach from the teams of Anna Nam and @lgmartelotto.bsky.social Preview: www.cell.com/cell-genomic... Paper: www.cell.com/cell-genomic...

New revolutionary hack about to change our molecular tool-box! Thanks @ioavlachos.bsky.social for sharing this! Rapid evolution of a highly efficient RNA polymerase by homologous recombination www.nature.com/articles/s41...

Join us to the last #GESTALT seminar the year! Free for all GESTALT member. If you are interested in anything #spatialbiology come be part of our growing community GESTALT Seminar Series globalspatial.org/seminar-seri... December 19 10am EST @ioavlachos.bsky.social @lgmartelotto.bsky.social

Thanks @natbiotech.nature.com for the shoutout and supporting our work at attempting QC in this emerging field of #spatial #transcriptomics @lgmartelotto.bsky.social @christophermason.bsky.social

Goliath effort to help with standards in spatial trancriptomics-can only be more amazing when done with @lgmartelotto.bsky.social @christophermason.bsky.social 🚀 Paper: nature.com/articles/s4158… Touchstone portal: spatialtouchstone.org Spatial touchstone protocols: protocols.io/view/spatial-t���

Standardized metrics for assessment and reproducibility of imaging-based spatial transcriptomics datasets - @jasmineplummer.bsky.social @lgmartelotto.bsky.social go.nature.com/4rBC1Z0

Calling all #singlecell #spatial officianados. Join GESTALT team in our workshop at NextGenOmics Spatial & Data 2025 Naveed Ishaque Musa Mhlanga Silas Maniatis Ahmet Coskun Free registration for GESTALT members Nov 13-14 in London UK! @jasmineplummer.bsky.social @ioavlachos.bsky.social

iSCALE www.nature.com/articles/s41...

Must read from Nick Navin’s lab! www.cell.com/cell/abstrac...

New online! Imaging-based multimodal profiling of single cells with STAMP

Very proud of these two! Anna Pascual Reguant | Kellie Wise A beautiful collaboration with Jasmine Plummer & Holger Heyn CNAG | St Jude Children’s Research Hospital #STAMP Imaging-based multimodal profiling of single cells with STAMP | Nature Reviews Genetics www.nature.com/articles/s41...

Counting my lucky stars⭐️that not only do I get to work with these amazing scientists Jiwoon Park @christophermason.bsky.social but also give fun @cshlnews.bsky.social lectures together💪.Big thank you to @lgmartelotto.bsky.social Nicholas Navin Julea Vlassakis for uniting this #singlecell #dreamteam

STAMP brings us non-destructive single-cell profiling without the sequencing www.omic.ly/stamp-brings...

Tracing colorectal malignancy transformation from cell to tissue scale www.biorxiv.org/content/10.1...

Congrats @hoheyn.bsky.social , @lgmartelotto.bsky.social and team!

Don’t forget to register to the seminar on June 27 and July 25: GESTALT - Seminar Series June 27 - 2025: Dr. Fei Chen July 25 - 2025: Dr. Hanchen Wang Please visit the Seminar Series Webpage for more information and registration: globalspatial.org/seminar-seri...

Conceptualised by @hoheyn.bsky.social @jasmineplummer.bsky.social @lgmartelotto.bsky.social Brought to life by Emanuele Pitino, Felipe Segato Dezem, Kellie Wise, Irepan Salvador Martinez, @helucro.bsky.social, Max Ruiz, Juan Nieto Sachica, Ginevra Caratu and more! 🎇🙏🏽👏🏽

👩‍🔬The lead authors of the study are Dr. @pascual-reguant.bsky.social & Emanuele Pitino (CNAG) with Felipe Segato Dezem (@stjuderesearch.bsky.social) Corresponding authors: Dr. @hoheyn.bsky.social (CNAG), Dr. @jasmineplummer.bsky.social (St. Jude) & Dr. @lgmartelotto.bsky.social (Univ. of Adelaide)

STAMP: Single-cell transcriptomics analysis and multimodal profiling through imaging @cellcellpress.bsky.social @lgmartelotto.bsky.social @hoheyn.bsky.social www.cell.com/cell/fulltex...

“STAMP allows us to profile a million cells simultaneously, compared to tens of thousands typical of current methods, making it far more scalable,” said @jasmineplummer.bsky.social, PhD, St. Jude Center for Spatial Omics director.

STAMP:Single cell Transcriptomics Analysis & Multimodal Profiling-cheap scprofiling-MILLIONS of cells, multimodal, pertubation expts & tissue atlas'ing at scale.Thx to brilliant colleagues @lgmartelotto.bsky.social Holger Heyn & our amazing teams. @cellpress.bsky.social www.cell.com/cell/abstrac...

Awesome work by awesome people! Endless innovation by @lgmartelotto.bsky.social and team. Can’t wait to try it 💪

Time to join GESTALT – Global Alliance for Spatial Technologies – GESTALT – Global Alliance for Spatial Technologies Together we do more … www.nature.com/articles/s41... globalspatial.org

Check the #AISG2025 speaker lineup! 🤩 Prof Jim Johnson Prof Jenny Gunton Prof Shane Grey A/Prof Tracey Putoczki Prof Anand Hardikar A/Prof Sumaira Hasnain Prof Wayne Hawthorn Professor Henry Pleass A/Prof Luciano Martelotto @jimjohnsonsci.bsky.social @lgmartelotto.bsky.social

🚀 Interested Spatial Omics with R/Bioconductor? 🧨Do you want to learn how to analyse these data in R with the #tidyverse? Join us in May for this course with @stemang.bsky.social & @lgmartelotto.bsky.social 🔗 www.physalia-courses.org/courses-work... #SpatialOmics #Bioinformatics #RStats