More structural biology! From the @jburkevic.bsky.social lab with work from @sushantsuresh.bsky.social and @alexlouiseshaw.bsky.social - featuring data collected in our facility! #cryoEM www.jbc.org/article/S002...

Final part of my PhD work is now out! We were able to develop a nanobody that inhibits EFR3 mediated PI4KA membrane recruitment. Potential therapeutic idea? Amazing collaboration with different labs using a host of different techniques. @alexlouiseshaw.bsky.social

New preprint from the lab headed up by @sushantsuresh.bsky.social! Late last year, we published the molecular basis of PI4KA plasma membrane recruitment by EFR3 (PMID:39705356). This work builds on these findings and identifies a new potential therapeutic avenue! Check it out!

Cool new paper (www.jbc.org/article/S002... from my lab spearheaded by graduate students Sushant @sushantsuresh.bsky.social and Alex @alexlouiseshaw.bsky.social . This was a very cool collaboration with the Scott Hansen, Jennifer Cobb, Tamas Balla labs

Thanks to the UBC media team for coverage of our statin : RyR1 work. Atorvastatins bind and trigger opening of a calcium release channel critical for muscle contraction. news.ubc.ca/2025/11/ubc-... @ubcmedicine.bsky.social

Congratulations to @filipvanpetegem.bsky.social on their latest paper- with a structure of ryanadine receptor bound to statins! #cryoEM www.nature.com/articles/s41...

Our latest work : Statins are used to lower plasma cholesterol but often come with muscle-related side effects. Using cryo-EM, we show how multiple statin molecules cooperate to bind RyR1, a calcium release channel mainly found in skeletal muscle. nature.com/articles/s41...

Check out this paper, for which we collected data for Rashmi Panigrahi and the Glover lab at UAlberta! We needed a tilted collection to get the side views of these nucleosomes, and it was a lot of fun working with our colleagues from another province! pubmed.ncbi.nlm.nih.gov/41123210/ #cryoEM ❄️🔬🇨🇦

We are excited to share the recent paper from @filipvanpetegem.bsky.social featuring cryo-EM structures of the KCNQ1 potassium channel collected at HRMEM! #cryoEM #structuralbiology www.nature.com/articles/s41...

Our cryo-EM structure of the intermediate state of the KCNQ1 potassium channel is out. Collaborative effort with the David Fedida and Luca Maragliano labs. Cryo-EM work spearheaded by Efthimios Kyriakis, PhD www.nature.com/articles/s41...

HRMEM is excited to share this new paper from the Cullis lab! Congrats to Miffy Cheng et al! www.nature.com/articles/s41...

HRMEM is excited to spotlight this excellent review of cell envelope diversity and evolution from HRMEM cryoET user Eltiza Tocheva (@elitzatocheva.bsky.social‬) and team. www.nature.com/articles/s41...

HRMEM is happy to spotlight this exciting work, which applied a magnetic separation technique to characterize LNP bimolecular coronas. Congratulations to Valentina Francia, Yao Zhang and colleagues from the Cullis lab at UBC. We are proud to have supported this work! 🔬 doi.org/10.1073/pnas...

In case you missed our May webinar, it is now available!! Ellen Zhong from Princeton University presented CryoDRGN-AI. Topic: CryoDRGN-AI: Neural ab initio reconstruction of challenging cryo-EM and cryo-ET datasets Host: Pete Meyer Recorded on: May 20, 2025 buff.ly/jKjWTOa #SBGridWebinars

HRMEM is proud to spotlight this structure of the cardiac potassium channel, KCNQ1, bound to its activator, ML277! Shout to Katrien Willegems, Jodene Eldstrom, Efthimios Kyriakis, Fariba Ataei and researchers from the Fedida and Van Petegem Labs at UBC! 🔬🫀🧬 #cryoEM www.nature.com/articles/s41...

HRMEM is happy to spotlight this structure of calcineurin bound to PI4KA determined by Alexandria Shaw and colleagues from the Burke Lab at the University of Victoria and the Yip Lab at UBC! We are proud to have provided the electron microscopy for this work! Read more: doi.org/10.1016/j.st...

Mass Spec + lasers + cryoEM = COOL SCIENCE! New research from @coonlab.bsky.social and the Grant Lab combines mass spec to land proteins on TEM grids, a laser to restore protein structure, and cryoEM imaging and analysis to produce 3D reconstructions. www.mcponline.org/article/S153...

Hey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪

We are happy to spotlight these structures of human ABCA4, a crucial transporter for ocular function, determined by Jéssica Fernandes Scortecci and colleagues from the Molday and Van Petegem Labs at UBC! 🔬 We are happy to have supported this work! #cryoEM Read more: www.nature.com/articles/s41...

Cryo-ET of Thermotoga maritima in a study led by Danielle Sexton and colleagues from the Tocheva Lab at UBC broadened our understanding of outer membrane biogenesis! Shout out to @elitzatocheva.bsky.social ! Read more about this awesome work supported by HRMEM here: www.pnas.org/doi/10.1073/...

🚨New preprint!🚨 #teamtomo We expanded Surface Morphometrics to quantify membrane thickness from cryo-ET—revealing local variation across organelles. Led by the lab’s first grad student, @mmedina300kv.bsky.social (defending Monday! 🍾) w/ @attychang.bsky.social @hamid13r.bsky.social & @tomo.science

Bin Zhao and colleagues from the Cullis Lab at UBC have developed a technique to determine the pH inside LNPs using a pH-sensitive fluorescent dye-based DNA probe. 🧬🔬 HRMEM is happy to have supported the electron microscopy for this exciting research! Read more : doi.org/10.1016/j.bi...

"Not only will it provide sharper details of proteins and other molecules imaged with cryo-EM," says @berkeleylab.lbl.gov 's Peter Denes, "but it should enable microscopists to image even proteins roughly half the size of what is currently possible."

We are excited to spotlight this structure of the type 3 ryanodine receptor determined by Yu Seby Chen and colleagues from UBC and the University of Siena! It was a pleasure to collaborate with the Van Petegem (@filipvanpetegem.bsky.social) Lab on this lovely work! www.nature.com/articles/s41...

HRMEM is happy to have supported this structure determination of Rab3GAP by Gage Fairlie, Kha Nguyen and colleagues. Congratulations to the Burke Lab (@jburkevic.bsky.social )at the University of Victoria and the Yip Lab at UBC! 🔬🎉 Read more here: www.nature.com/articles/s41...

Congratulations to Franco Li, Shaun Peters and colleagues from the Strynadka Lab at UBC for their recent publication showcasing cryo-EM structures of S. aureus TarGH in the presence of targocil-II! HRMEM is proud to have supported this work! 🔬🫶 www.nature.com/articles/s41...

People always ask me what resolution they need and I say...depends on the question you're asking. This is a really cool example of that extra resolution being meaningful!

In @justinditrani.bsky.social final postdoc paper (with contributions from many others) we show that forming/purifying vesicles (here from M. smegmatis) allows structure det'n of *endogenous* membrane proteins in their *native lipid bilayer* 🧵 on methods & TB biology... www.pnas.org/doi/full/10....

Millin' like a villain

Bringing together tardigrades and #CryoEM, I love it! ❤️ www.biorxiv.org/content/10.1... 🧪

OK this is COOL i love it www.biorxiv.org/content/10.1...

A very cool method by Bonnie Murphy's lab to map elements for cryo-EM. The method, named REEL-EM, makes use of electron-energy loss spectroscopy. Honored to have contributed to this: www.biorxiv.org/content/10.1...