Freddy Boutrot (@freddyboutrot.bsky.social)

Phytophthora targets plant extracellular vesicles to promote infection a and b, TET8, TET9, PEN1, but not ARA6 could be detected in total extracellular vesicles (P100 pellet) of Arabidopsis. a, eGFP labeled proteins and plant actin were detected by western blotting. b, Confocal microscopy of EVs isolated from TET8-eGFP, TET9-eGFP, ARA6-eGFP and eGFP-PEN1 expressing Arabidopsis respectively. Bar, 10 µM. c, EVs were isolated from TET8-eGFP expressing lines. AWF (without EVs) were prepared by ultracentrifugation at 100,000 × g for 6 hours. Proteins were detected by western blotting. d, Schematic representation of purification of plant EVs by Size Exclusion Chromatography (SEC). e, Elution fractions of Size Exclusion Chromatography were collected. TET8-eGFP, TET9-eGFP and eGFP-PEN1 were detected by western blotting. Dashed box indicates collection of EV fractions for further analysis. BV (Buffer Volum). f, Transmission electron microscope (TEM) observation of EVs fraction (Elution: 0.496-0.540 ml). Bars, 200 nm. g, Nanoparticle tracking analysis (NTA) of EVs fraction (Elution: 0.496-0.540 ml) was collected. Graph shows distribution of particle size intensity. h, SEC-EV particle number corresponding to different protein concentrations. EV abundance was quantified by NTA, with particle count representing EV quantity. i, TEM observation of soybean EVs (Size Exclusion Chromatography purification, same fractions with Arabidopsis EVs). EVs from soybean leaves were isolated. j... https://www.nature.com/articles/s41564-026-02325-3