Nick Talbot (@talbotlabtsl.bsky.social)

Nat Plants: New alleles of Arabidopsis BIK1 reinforce its predominant role in pattern-triggered immunity and caution interpretations of other reported functions (2026) The receptor-like cytoplasmic kinase BIK1 and its close homologue PBL1 have been widely recognized as central components of plant immunity. However, most genetic studies of BIK1 and PBL1 functions were carried out with single transfer DNA (T-DNA) insertional mutant alleles. Some phenotypes observed in these mutants, for example autoimmunity, have been difficult to reconcile with the proposed role of BIK1 and PBL1 in pattern-triggered immunity. In this study, we generated several new alleles of bik1 and pbl1 by CRISPR–Cas9-based gene editing and systematically analysed these mutants alongside existing T-DNA insertional lines. These analyses reinforced the central role of BIK1 and PBL1 in pattern-triggered immunity mediated by both receptor kinases and receptor-like proteins. At the same time, however, we revealed several pleiotropic phenotypes associated with T-DNA insertions that are not necessarily linked to loss of BIK1 or PBL1 function. Further analyses of newly generated bik1 pbl1 double mutants uncovered an even greater contribution of these kinases to immune signalling and disease resistance than previously appreciated. These findings clarify longstanding ambiguities surrounding BIK1 and PBL1 functions. Thorough genetic analyses by four laboratories reinforce the central role of the protein kinase BIK1 in plant immunity. Other unrelated functions proposed for BIK1, however, are associated with genome rearrangement in a single transfer DNA mutant. https://www.nature.com/articles/s41477-025-02187-3?utm_medium=social&utm_source=bluesky