Congratulations to now graduated PhD student Stephen Winston for leading this study on rAAV contaminants. This study shows that a mere 2 nucleotide modification to the (Rep78/68 driving) p5 promoter results in rAAV with equivalent titers but ~50-fold lower p5-derived contaminants. #genetherapy #AAV

Starting 2026 with a thread a very recent manuscripts at Ryan Lab @tcddublin.bsky.social @tbsi-tcd.bsky.social "Extinction and subsequent updating of innate fear responses to a visual looming stimulus rely on hippocampus-dependent mechanisms" journals.plos.org/plosbiology/... @PLOSBiology

Two complementary studies from our group out today: a therapeutic peptide vaccine trial for fibrolamellar carcinoma (FLC) & TIRTL-Seq, our robust method for deep quantitative paired TCR sequencing a🧵w/ @markyarchoan.bsky.social & @pogorely.bsky.social www.nature.com/articles/s41...

How do we balance innate responses & adaptive learning when reacting to threats? @tjryan.bsky.social &co show that repeated exposure to looming stimuli reduces innate defensive responses via #hippocampal modulation, revealing a vCA1 engram needed for learned #fear @plosbiology.org 🧪 plos.io/4pNSnNp

So glad to see this story ready to share as a preprint. This was a really fun collaboration and a stunning finding with big potential implications. Congrats Iva!

Opposing effects of H2A.Z on memory, transcription and pathology in male and female Alzheimer's disease mice and patients https://www.biorxiv.org/content/10.1101/2025.05.28.656659v1

*You have a new citation, click here to view* Citing article: 'In contrast to a pointless 2024 study done by barely literate and grotesquely unattractive authors [12]'

Had an excellent meeting with staff from @slotkin.senate.gov about the importance of supporting robust funding for Biomedical research. @immunologyaai.bsky.social and the community appreciate your support #PPFPHillDay

Plasticity of visual looming response reveals a dissociation of innate and learned components https://www.biorxiv.org/content/10.1101/2024.12.18.628938v1

Durable lymphocyte subset elimination upon a single dose of AAV-delivered depletion antibody dissects immune control of chronic viral infection @cp-immunity.bsky.social www.cell.com/immunity/ful...

An amazing collaboration to be a part of: We helped @gisellavetere.bsky.social & @clementpouget.bsky.social simultaneously deliver 3 AAV constructs to Ca1 neurons. What they then acheived blew me away - the tagging of cells recruited in memory encoding with a temporal resolution of mere seconds!

We keep improving TIRTL-seq, see the updated preprint (www.biorxiv.org/content/10.1...)! New additions include: a manual 96-well protocol, code and raw data now available, and successful in vitro validation of EBV and SARS-CoV-2 TCRs.

New starter pack for all people interested in Gene Editing & Gene Therapy 🧬✂️ including Tools & Delivery|CRISPR-Cas|Zinc finger nucleases|Viral vectors|Non-viral delivery methods go.bsky.app/8R4dVez

Glad to get the chance to write this commentary on a recent paper by Penaud Budloo et al that found miRNA contaminants within purified rAAV preparations. An interesting and unique AAV vector purity study

TCR repertoire dream: decoding immune status by sequencing TCRs. Problems: 1) Need paired TCRab 2) Interesting T cell clones are often VERY small. Our solution: TIRTL-seq 🐢Deep, quantitative, & affordable paired TCR repertoire sequencing🧵 https://www.biorxiv.org/content/10.1101/2024.09.16.613345v1

Not only is the alternative histone H2A.Z.1 involved in memory formation, this role is lysine acetylation dependent! Thrilled to have contributed to this great work from Iva Zovkic's lab