Molecular Therapy Family of Journals (@moltherapy.bsky.social)

RT-qPCR and split-luciferase methods enable batch standardization and analysis of Base Editor engineered Virus-Like Particle transduction events Recently developed engineered Virus-Like Particles (eVLPs) have emerged as a promising delivery vehicle for ribonucleoprotein gene editing complexes. Variability in eVLP batch production may, however, hinder reproducibility and standardization across pre-clinical investigations designed to characterize and optimize this platform. Ultimately, stringent production, purification and quantification processes will be required for this technology to reach the clinic. In this study, we developed two titration methods for base editor (BE)-eVLPs: a spacer-agnostic RT-qPCR assay to quantify the copy number of sgRNA molecules, and a NanoBiT® Luciferase-based approach to estimate VSV-G glycoprotein abundance, per μl of BE-eVLP preparation. http://dlvr.it/TQrTPF