My favorite tool #Opencloning.org has just released a feature for assembling golden gate cloning fragments. I have not used it **yet**, but I've used the In-Fusion tool, and that ones has worked amazingly. Give it a try! I am sure @manu-lera.bsky.social will be happy to hear from your experience.

I got a plasmid map from another lab as *.dna file 🧪. The SnapGene program can open it. I do not want to install their "free" viewer in my linux system. The solution: OpenCloning opencloning.org . With it, I loaded the .dna file and downloaded the sequence plus a nice map. Thanks OpenCloning team 🤩

Finally 🥳. Our resource paper under Open Science and FAIR data principles 🧪. I never thought that a simple resource paper will take more than 1 1/2 years to get reviewed. But now it is finally out bmcresnotes.biomedcentral.com/articles/10....

🧪Our first paper with Machine Learning under Open Science principles and FAIR data is out! rdcu.be/ezXFx

You have analized your data with many steps. Do you want to make the analysis reproducible for others and your future-you? If you are in the LMU Munich, you can come to our workshop on *May 6th*. We will show you how to create workflows in R. Www.pretix.osc.lmu.de/lmu-osim/hig... 🧪

More of this, please. Welcome to Bluesky, finally crawling out of the Upside Down? No Demogorgons here, the forecast is clear, the science sharper, and the community healthier. www.reuters.com/world/europe...

« Assessing the feasibility and impact of clinical trial trustworthiness checks via an application to Cochrane Reviews: Stage 2 of the INSPECT-SR project » by a gang of researchers led by @jackwilkinson.bsky.social

Iron is essential for growth of Tetrahymena in axenic media (prey-free)🧪. Last time we made our stock solution was three years ago. How do we prepare it and store it? The details are now published in @protocolsio.bsky.social under www.protocols.io/view/fecl3-s... and just in time for a new lot 🥼🫡

Buffer is ready. Hmm. can I sterilize it in the autoclave? When the answer is no, the next best option is to do a sterile filtration. It's not a big deal, but it's easy to do it wrong. Here is our protocol! 🧪 Sterile filtration of liquid solutions  www.protocols.io/view/sterile...

Nothing more annoying than preparing buffers, but extremely necessary if we want to mate ciliates. Here is our protocol for the 1 M Tris-HCl pH 7.5 for Tetrahymena mating (Stock solution) www.protocols.io/view/1-m-tri.... We hope it will be helpful for the community! 🧪 🤲🏻🥼

I love to help to solve challenges that come up in the lab, and yesterday I was able to link our protocol for veggie NEFF media in a personal communication (www.protocols.io/view/veggie-...) while discussing differences in flow cytometry results. Life is easier with @protocolsio.bsky.social 🤪🧪

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We still have places in our Machine Learning School. Anyone in natural sciences or medical background can take part. You just need to be in Munich between the 9th and 12th of December and have the full day available! You will learn the basic of python and will apply it to create your own ML model 🧪

Two more protocols out in @protocolsio.bsky.social. Little by little we can build upon each one to get everything ready for the big ones. This time we have a buffer solution for Quality Control of the AlamarBlue stain, and a classic: Gram staining www.protocols.io/view/gram-st....

Question to the community 🧪: in the last 4 years, I have tutored PhD students from other labs and institutions. Too many have had a lot of problems because of bad PIs, and I do mean really bad scientist and people. Some are under investigation. How can I warn future students about them?

First-of-its-kind Online marketplace for sharing tissues, organs and other biological materials. @science.org By @arukon0.bsky.social #scisky #news #science www.science.org/content/arti...

These are outstanding resources for anyone engaged in creating scientific knowledge. 🧪🛟🩺

www.linkedin.com/posts/eric-p... Really interesting view... how would you measure trustworthiness? 🧪

At erc.europa.eu/news-events/..., they link a document with the list of criteria on how to evaluate if a data repository complies with the new Horizon Europe grants. A list of trusted repositories should be in the document, too 🧪. Make you data FAIR!

The plan is to make all our protocols available for the community. Thanks to my team and their hard work, the first one is out! www.protocols.io/view/tb-medi.... Thanks to @protocolsio.bsky.social for such a great platform. 🧪

Do you want to start to see your data from the point of view of Machine Learning? Look into our course description under mlschool.rostlab.org and come if you are interested. We have places open and it is free! Our focus is biological sciences 🧪

Curious about how you can use your data in the 21st Century? Come and learn with us between the 9th and 12th of December about Machine Learning (mlschool.rostlab.org). After you learn the basics of python, we dive into tidy (FAIR) data, and your first model under best practices in the field. Free!!!

The fun starts now! Medical students are coming out, and we are ready for them!

Tod@s podemos ayudar a transformar ciencia a Ciencia Abierta! Publica tus protocolos de laboratorio en detalle en @protocolsio.bsky.social. Una vez publicados se pueden referenciar con el DOI! Así es mas fácil explicar métodos en tus publicaciones y tesis! Incrementa tu visibilidad! 🧪

For everyone in Munich or with an option to stay in the area in December: we have still places in our Winterschool for Toxicologist -Machine learning version (December9th to 12th). Check it out... and it is free. Mlschool.rostlab.org

And yes, we did just add "share on @bsky.app" to @protocolsio.bsky.social just as @altmetric.com recommended bsky.app/profile/altm.... (Thank you for the nudge @exotoxinslab.bsky.social!)

You can do it too! Start promoting Open Science by publishing detailed protocols with the help of @protocolsio.bsky.social. Very happy to help with the visibility of protocols made available by other communities! Veggie NEFF media for Tetrahymena thermophila www.protocols.io/view/veggie-...