(1/n) DNA-PAINT imaging inside the nucleus at single antibody resolution using TIRF? Ultrathin sectioning makes it happen! Grateful to share my postdoctoral work introducing “tomographic & kinetically-enhanced DNA-PAINT” or in brief: tkPAINT. Out in @pnas.org! doi.org/10.1073/pnas... 👇🧵

Astronomy Picture from 21/04/2008 Bacteriophages: The Most Common Life-Like Form on Earth Source: https://apod.nasa.gov/apod/ap080421.htm#Bacteriophagesi#Viruses##ElectronMicroscopyc#Microbiologyo#Astronomys#Spacey#Science##BacteriaB#Phages #TinyWorldsnyWorlds

Apply for a position as Postdoctoral Research Fellow in Fish Immunology/Microscopy @biovitenskap.bsky.social This role contributes to the ERC-funded project “Fish-S.H.I.E.L.D.”, which investigate the fish immune surveillance system and its importance for the fish defense against infectious diseases

We are proud to announce the launch of FAIR Image Analysis Across Sciences, supported by the OSCARS project, in collaboration with Simula Research Laboratory, #EPFL & University of Bergen (UiB)! 🎉

Vi har mange ledige #PhD-stillinger ledige ved @biovitenskap.bsky.social med søknadsfrist 28. februar 2025 Kanskje noen av stillingene er aktuelle for deg eller noen du kjenner? Ta en titt og del gjerne! www.mn.uio.no/ibv/om/ledig...

Hyperparasitisme : un virus qui parasite un virus qui infecte une amibe

We have a PhD position available at the Department of Biosciences, University of Oslo to work on peptide signaling in plants and insects. Please share. www.jobbnorge.no/en/available...

For the first time in plants, we used APEX-based electron microscopy to map the precise localization of ATG8 at the vacuolar membrane after stress! 🚀 Pushing the boundaries of plant cell biology—one EM image at a time. ⚡👀 #PlantScience #ElectronMicroscopy #Autophagy #Vacuole #NaturePlants

#Correlative Light and Electron Microscopy, #CLEM, is a modern #method combining light and #electronmicroscopy data. Although it is considered “new”, it was already used in the early 1960s, for instance to study the #ultrastructure of mitotic cells in this beautiful example: doi.org/10.1083/jcb....

sauerkraut with a variety of bacteria magnified 8,000x

I fear there is deep truth here.

“One of the challenges in science journalism is the oversimplification of research findings to attract attention. Headlines such as “scientists find cure for cancer” or “ozone layer is healing” are designed to attract readers’ attention but often misrepresent the complexity of scientific research.”

This is probably what a #rotifer gets to see when it encounters a (seemingly bad-tempered) 6dpf #zebrafish larva. #Creepy! But maybe the fluffy #nostrils or the funny #neuromast beard make the situation a little better... Scanning #electronmicroscopy / #SEM

Still our favorite #review! Unfortunately, as relevant today as when it was published, it is an excellent illustration of the #problems arising from the neglect of #ultrastructure and #electronmicroscopy in #cell-biology and the over-reliance on #fluorescence #microscopy. doi.org/10.1016/0962...

New #ISEPpapers! The nature of ‘jaws’: a new predatory representative of #Provora and the ultrastructure of nibbling protists royalsocietypublishing.org/doi/10.1098/... #Protists #Microbes #Biology #TreeOfLife #Microscopy

#Science in the age of #selfies A short #opinion article that we think hits a very important point! Albert #Einstein: “an academic career, in which a person is forced to produce #scientific writings in great amounts, creates a #danger of intellectual superficiality” www.pnas.org/doi/10.1073/...

Was gifted this one for xmas: www.goodreads.com/book/show/26..., looking forward to reading 👀

Dynamic duo at the cell's edge: Caveolae and the actin cytoskeleton work together to sense and respond to mechanical stress. Caveolae buffer tension, while actin provides structure and force. Ultimate biomechanics team. #CellBiology #Mechanotransduction

Instead of listing my publications, as the year draws to an end, I want to shine the spotlight on the commonplace assumption that productivity must always increase. Good research is disruptive and thinking time is central to high quality scholarship and necessary for disruptive research.

At my institution, they want to install profiles on our personal phones that will give IT root level access to our personal phones. And allow them to remotely wipe our phones. Our personal phones. Damn straight, take your work email off your phone. And remove your work profiles from your phones.

Safer, nonradioactive staining alternatives for electron microscopy are introduced, simplifying sample preparation, reducing costs and making structural biology more accessible to researchers globally #NegativeStaining #ElectronMicroscopy doi.org/10.1107/S205...

This is our IT department telling us that we cannot copy confocal microscopy data to our Imaris workstation so that we can... analyze the confocal data.

Did you know that just 100-200 gold particles in your #immuno-EM data can give you an incredibly good idea of the #labelling distribution and reveal positive signals that you may not immediately recognise? It's amazing how reproducible this " #100-gold-method" is. #stereology doi.org/10.1369/jhc....

Join us for the last Pub before the Winter Break! ☃️ We'll learn about #VolumeEM approaches from the experts at #EuroBioImaging’s Advanced Light & Electron Prague Node, part of @volumeem1.bsky.social community. 🗓️Fri, Dec 20 @ 13:00 CET All are welcome 🔽 www.eurobioimaging.eu/events/diver...

I wrote some thoughts about why peer review matters It shapes scientific standards, maintains field coherence & trains new researchers Yes, it needs improvement—but it's the glue that holds scientific progress together briscoelab.org/2024/12/11/i...

🧪🎅 12 Days of Tools for Science Management 🦠🎄 Day two: pubcrawler.gen.tcd.ie Written by the amazing Karsten Hokamp, Pubcrawler is a customizable alert system for newly-published manuscripts that gives you power over type and frequency of alert. Many other tools exist, but none with as much neon!

The toxic, #arsenic -containing #Cacodylate buffer is still used in many #electronmicroscopy labs today. Although it is a good buffer, it is no longer needed today as there are better and safer alternatives that perform just as well - #PHEM buffer (Schliwa, 1981) for example. doi.org/10.1111/j.13...

Not a fish or a dragon scale, but a single cell of a #protistsonsky. This rhizarian testate amoeba creates perfect tiny glass scales to build it's shell. From a peat bog. #protistaday.

I'm gonna piss a few nerds off but this is an example of why a lightsheet microscope should be used to study organoids. The detection optics are great for imaging in XY but they suck axially. Sure, yeah, hocus pocus, RI matching, correction collar, blah blah, but it's physics, baby.

Last week I showed to MSc students this iconic movie of a #leukocyte chasing a #bacteria, just wait... Version taken from a 16 mm video made by David Elliot Rogers @VanderbiltU in the 1950s ! (as @mag2art.bsky.social says "the Citizen Kane of #Microscopy) #CellMigration @focalplane.bsky.social

Last-minute Christmas shopping? Not for you! We recommend “Remarkable. What animals can teach us about ourselves, the value of diversity, and saving the world,” by @mdc-berlin.bsky.social science writer Russ Hodge and artist Kat Menschik! More details: www.mdc-berlin.de/news/news/wo...

It's #FluorescenceFriday and we celebrate the #HistoryOfMicroscopy: The paper that introduced PAINT was published on the 12th of December 2006 in the journal PNAS. Read the historic paper: t.co/fw3cQrnEhX #SuperResolution #HistoryOfScience #SMLM #Nanoimger #Microscopy

Hi Bluesky community! 👋 Are you looking forward to 2025 as much as we are? 👀 We just updated our 2025 Annual Poster to include even more events for you to join, so go and have a look! ➡️ s.embl.org/poster-bl #EMBLEvents #molecularbiology #lifesciencetraining

We are rather skeptical about conclusions based on #AI-driven / #deeplearning #image #analysis (not towards an application as a tool). This viewpoint is supported by a recent study on medical imaging, which we believe can be applied to #bioimage data in general. #microscopy doi.org/10.1038/s415...

It's truly surprising that the #tokuyasu method is so underutilized in #fluorescence #microscopy! It eliminates the need for permeabilisation and increases Z resolution to 50nm on any microscope. It is also the method used in the Nobel Prize-winning work on #super-resolution. doi.org/10.1177/0022...

It is truly remarkable that Fritiof Sjöstrand was able to draw such precise conclusions about the cell in the era of primary osmium tetroxide #fixation, preceding the introduction of glutaraldehyde by Sabbatini in 1963. This 1956 review is a masterpiece of #ElectronMicroscopy doi.org/10.1016/S007...

We organized a symposium titled "A Rab, a Rab, my kingdom for a Rab" to honor one of our founding directors Marino Zerial: www.mpi-cbg.de/news-outreac...

As we recently posted, our preferred #fixation #buffer for mammalian cells and most tissues is PHEM. Marine invertebrates, especially #plankton, seem to fix better with a variation, as shown in this publication by Montanaro. Of course, osmolarity has to be considered as well. doi.org/10.7717/peer...

I am pleased to be sharing my work in the @rouxlab.bsky.social at ASCB & EMBO Cell Bio 2024! If you’re interested in membrane bending, crowding, curvature and of course the ESCRT machinery, pass by my talk on Saturday, Dec. 14th and/or my poster on Tuesday, Dec. 17th! #CellBio2024

Always find this chilling to read given what we know now and how many people over several years were unknowingly exposed to and died of HIV / AIDS (1/2) #IDSky #HIVSky #MedSky

An exhibit that opened last weekend at Yale shows drawings from the influential duo Santiago Ramón y Cajal and Camillo Golgi, recasting them as collaborators rather than rivals. It also reveals lessons for modern scholars. By @claudia-lopez.bsky.social www.thetransmitter.org/art-in-scien...

Given the love for Sydney Brenner whenever I post stuff about him, here’s a link to a BBC World Service programme I made about him, which included a visit to interview him in Singapore.

Electron microscopy images and morphometric data of SARS-CoV-2 variants in ultrathin plastic sections www.nature.com/articles/s41...

Clathrin-coated pits working overtime in your heart this weekend🔬 #ClathrinLife #WeekendScience"

The Umeå Center for Electron Microscopy (UCEM), part of #EuroBioImaging 's Swedish NMI Node, has a new FIB-SEM system! Congratulations! 🎉 www.umu.se/en/news/new-...

I created a starter pack for Imaging Scientists that work in core facilities. I'm sure I missed lots of people since I searched for those that have "core" or "facility" in their bios, so let me know who else should be added! go.bsky.app/2bbgRQU

A byproduct of a project on the #fish #immunesystem with Julien Resseguier from IBV @biovitenskap.bsky.social. The colored #SEM image shows a cross-section of the #gill #vasculature and the position of a #rodlet-cell (green) in the epithelium including its surface protrusion. doi.org/10.1126/scia...

Hard to believe that these photos represent brain samples from 8 different healthy donors. #ImageForensics Published in Nature, 2022. Cited 449 times according to Dimensions. doi.org/10.1038/s415...