Lucas A. Meirelles (@la-meirelles.bsky.social)

Intracellular glutamine fluctuates with nitrogen availability and regulates Mycobacterium smegmatis biofilm formation | Journal of Bacteriology A subset of nontuberculous mycobacteria (NTM), including Mycobacterium abscessus, are opportunistic pathogens that can cause severe pulmonary infections. Biofilm formation renders M. abscessus more tolerant to antibiotics; hence, the ability to inhibit NTM biofilm formation could help us better prevent and treat NTM infections. However, the regulatory systems controlling NTM biofilm formation, which could include targets for anti-biofilm therapeutics, are poorly understood. The significance of this work is that it reveals intracellular glutamine as an important node controlling the initiation of biofilm formation in the model NTM Mycobacterium smegmatis. Building on this foundation, future studies will investigate how NTM biofilms can be dispersed by altering glutamine levels and will describe how NTM translates intracellular glutamine to the alteration of surface adhesins. https://journals.asm.org/doi/10.1128/jb.00252-25