Che-Fan Jeffrey Huang
@jfhorner.bsky.social
📤 92
📥 238
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A Taiwanese in Chicago 👨🔬📯🌈🧋🧋🧋 Proteome explorer, horn player and boba enthusiast!
I will be presenting at Consortium for Top-Down Proteomics’s Proteoform Thursday Webinar Series. The catenin phospho-code work laid the foundation for my future research group. Free register link below!
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1 day ago
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I am thrilled to share that I will be joining
@tamuchemistry.bsky.social
as a tenure-track Assistant Professor this summer. My research group will advance top-down mass spectrometry and proteomics to study the proteoform biology of cell–cell adhesion and phosphorylation in disease.
3 days ago
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reposted by
Che-Fan Jeffrey Huang
Consortium for Top-Down Proteomics
3 months ago
Join us January 22 for Proteoform Thursday: Pei Su of UC Riverside presents "From Tissues to Single Cells: Direct Proteoform Profiling Using Orbitrap-Based Single Molecule Mass Spectrometry"
#proteomics
#proteoform
#massspec
us06web.zoom.us/meeting/regi...
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Wonderful native individual ion mass spectrometry work!
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3 months ago
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It was a great pleasure attending my first
#HUPO
world congress in Toronto last week and sharing our catenin phospho-code story! It was inspiring meeting with peers and pioneers in the field, which reminded me how special the proteomics community is!
4 months ago
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THRILLED to share our new publication in
@angewandtechemie.bsky.social
from
#CLP
&
@nucdb.bsky.social
"Intact Mass Profiling Reveals Phospho-Proteoforms of the Catenins (85–110 kDa) Regulated by Actomyosin Contractility” using TDMS to study LARGE catenin proteoforms. (1/7)
doi.org/10.1002/anie...
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Intact Mass Profiling Reveals Phospho‐Proteoforms of the Catenins (85–110 kDa) Regulated by Actomyosin Contractility
This study advances top-down individual ion mass spectrometry (I2MS) to profile intact phospho-proteoforms of β- and α-catenins (85–110 kDa) within cadherin–catenin complexes. By modulating actomyosi....
https://doi.org/10.1002/anie.202518593
4 months ago
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Heading to
#HUPO2025
! I’ll talk about our catenin phospho-code story and how top-down MS reveals how adhesion proteins respond to actomyosin force! Thanks for the invite,
#AFFINISEP
! Full story on bioRxiv 👉https://doi.org/10.1101/2025.09.06.674621
5 months ago
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reposted by
Che-Fan Jeffrey Huang
PastelBio
10 months ago
Neil Kelleher: The Human Proteoform Project Could Transform Medicine
theanalyticalscienti...
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#proteomics
#prot-article
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reposted by
Che-Fan Jeffrey Huang
MSAID
11 months ago
🚨 New in
@naturemethods.bsky.social
#CHIMERYS
has already transformed
#proteomics
since 2022, powering DDA, DIA & PRM analysis in a single workflow. Wanna know how CHIMERYS works? 👉
www.nature.com/articles/s41...
#TeamMassSpec
#NatureMethods
#ASMS2025
🧪🔬 1/7
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reposted by
Che-Fan Jeffrey Huang
Nikolai Slavov
11 months ago
High-performance proteomics at any chromatographic flow rate These data follow the expected trends as though taken from a textbook ! They illustrate clearly the trade-offs between high and low flow rates.
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Sigh…
add a skeleton here at some point
11 months ago
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reposted by
Che-Fan Jeffrey Huang
Biology Open
11 months ago
🧪Phuong Le, Jeanne Quinn,
@jfhorner.bsky.social
@carajgottardi.bsky.social
& co show that the adhesion protein α-catenin has a key modification that allows dividing cells to stay better connected to their neighbours, helping the tissue stick together during mechanical stress.
doi.org/10.1242/bio....
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The mass spectrometry research community at Chicago is thriving! Many thanks to the Chicago Mass Spec Interest Group for the invitation to share my catenin proteoform story in the inaugural event. It was a pleasure to meet with researchers at the beautiful CZbiohub space!
11 months ago
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Looking forward to sharing my work at the Chicago Mass Spec Interest Group’s inaugural meeting!
11 months ago
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It was a very fruitful
#USHUPO2025
! I was beyond thrilled to share our advances in catenin proteoform profiling and functional studies that will reveal the phospho-code in cell-cell adhesions. Many thanks to the conference organizers and participants!
about 1 year ago
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reposted by
Che-Fan Jeffrey Huang
Craig M. Crews
about 1 year ago
Next steps for targeted protein degradation: Cell Chemical Biology
www.cell.com/cell-chemica...
#TPD
#PROTACs
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Next steps for targeted protein degradation
Modalities and applications of targeted protein degradation (TPD) have rapidly expanded the therapeutic possibilities of proximity induced pharmacology. Here, Krone et al. spotlight three focal points...
https://www.cell.com/cell-chemical-biology/fulltext/S2451-9456%2824%2900439-2
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Looking forward to attending the
#USHUPO
conference in Philly this weekend and sharing some early results in catenin proteoform profiling and our vision to build a story behind acto-myosin contractility regulation in cell-cell adhesion. Please join my talk next Wednesday and say hi!
about 1 year ago
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reposted by
Che-Fan Jeffrey Huang
Ben Garcia
about 1 year ago
New manuscript from the lab to establish a Top Down MS platform using electron activated dissociation on the Sciex ZenoTOF 7600 is out at the Journal of Proteome Research!
pubs.acs.org/doi/full/10....
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Establishing a Top-Down Proteomics Platform on a Time-of-Flight Instrument with Electron-Activated Dissociation
Top-down proteomics is the study of intact proteins and their post-translational modifications with mass spectrometry. Historically, this field is more challenging than its bottom-up counterpart because the species are much bigger and have a larger number of possible combinations of sequences and modifications; thus, there is a great need for technological development. With improvements in instrumentation and a multiplicity of fragmentation modes available, top-down proteomics is quickly gaining in popularity with renewed attention on increasing confidence in identification and quantification. Here, we systematically evaluated the Sciex ZenoTOF 7600 system for top-down proteomics, applying standards in the field to validate the platform and further experimenting with its capabilities in electron-activated dissociation and post-translational modification site localization. The instrument demonstrated robustness in standard proteins for platform QC, as aided by zeno trapping. We were also able to apply this to histone post-translational modifications, achieving high sequence coverage that allowed PTM’s site localization across protein sequences with optimized EAD fragmentation. We demonstrated the ability to analyze proteins spanning the mass range and included analysis of glycosylated proteins. This is a reference point for future top-down proteomics experiments to be conducted on the ZenoTOF 7600 system.
https://pubs.acs.org/doi/full/10.1021/acs.jproteome.4c00874
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reposted by
Che-Fan Jeffrey Huang
BioMassSpec
about 1 year ago
Standardized workflow for multiplexed charge detection mass spectrometry on orbitrap analyzers
#NatProtoc
www.nature.com/articles/s41...
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Standardized workflow for multiplexed charge detection mass spectrometry on orbitrap analyzers - Nature Protocols
Orbitrap-based individual ion mass spectrometry enables charge detection mass spectrometry application on a broadly accessible mass spectrometry platform, enabling the analysis of complex mixtures tha...
https://www.nature.com/articles/s41596-024-01091-y
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It was a tremendous pleasure to visit my alma mater National Taiwan University and give a talk on top-down proteomics and proteoform biology at the Institute of Biomedical Sciences earlier this week.
about 1 year ago
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reposted by
Che-Fan Jeffrey Huang
PastelBio
about 1 year ago
Advancements in Global Phosphoproteomics Profiling: Overcoming Challenges in Sensitivity and Quantification
analyticalsciencejou...
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#proteomics
#prot-paper
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reposted by
Che-Fan Jeffrey Huang
PastelBio
about 1 year ago
Emerging opportunities for intact and native protein analysis using chemical proteomics
www.sciencedirect.co...
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#proteomics
#prot-paper
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I love it when a place plays the Nutcracker during the holiday season, not All I Want For Christmas Is You on repeat 😝
about 1 year ago
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reposted by
Che-Fan Jeffrey Huang
BioMassSpec
about 1 year ago
Multi-Reflecting TOF MS for Analyzing Proteins
#IJMS
www.sciencedirect.com/science/arti...
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Multi-Reflecting TOF MS for Analyzing Proteins
This paper presents detailed results of previously reported protein studies conducted using a prototype multi-reflecting time-of-flight mass spectrome…
https://www.sciencedirect.com/science/article/abs/pii/S1387380624002008
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reposted by
Che-Fan Jeffrey Huang
BioMassSpec
over 1 year ago
Intact Mass Proteomics Using a Proteoform Atlas
#JProteomeRes
pubs.acs.org/doi/10.1021/...
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Intact Mass Proteomics Using a Proteoform Atlas
Top-down proteomics, the characterization of intact proteoforms by tandem mass spectrometry, is the principal method for proteoform characterization in complex samples. Top-down proteomics relies on p...
https://pubs.acs.org/doi/10.1021/acs.jproteome.4c00838
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Very happy to share my third major work this year (and first on 🦋)! This is a co-first work with the Fitzgerald group at Duke where they perform the SPROX protein folding stability assay and I figure out how to decribe the results using top-down proteomics!
doi.org/10.1021/acs....
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Top-Down Stability of Proteins from Rates of Oxidation (TD-SPROX) Approach for Measuring Proteoform-Specific Folding Stability
The crucial roles of proteoforms in biological processes and disease mechanisms have been increasingly recognized. However, the rate at which new proteoforms are being discovered using top-down proteomics has far outpaced the rate at which the functional significance of different proteoforms can be determined. Because of the close connection between protein folding and protein function, protein folding stability measurements on proteoforms have the potential to identify functionally significant proteoforms of a given protein. While a number of mass spectrometry-based proteomics methods for making protein folding stability measurements on the proteomic scale have been reported over the past decade, none have been interfaced with top-down proteomics. Described here is a top-down (TD) stability of proteins from the rates of oxidation (SPROX) approach for making proteoform specific folding stability measurements. This approach is validated using a mixture of three model proteins with well-characterized protein folding behavior by conventional SPROX as well as other more conventional biophysical techniques. The method is also used to evaluate the relative folding stabilities of the <30 kDa protein fraction isolated from an MCF-7 cell lysate. The relative folding stabilities of 150 proteoforms from 83 proteins were successfully characterized in the cell lysate analysis using the TD-SPROX approach.
https://doi.org/10.1021/acs.analchem.4c04469
over 1 year ago
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Is “proteomics approach” the same thing as “proteomic approach”? I was under the impression that you should always add the s at the end of -omics, even as an adjective because it’s part of the word to show that we’re measuring thousands of targets at once. But I see people using proteomic without s.
over 1 year ago
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Going home from the lab be like 🥶. First snow is Chicago!
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over 1 year ago
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I’m so glad being in Chicago where I get to enjoy the finest musicians playing in concerts. Photo from Daniil Trifonov’s piano recital this afternoon at Chicago Symphony Orchestra!
over 1 year ago
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Here we go on another social media platform!
over 1 year ago
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you reached the end!!
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