Jesper Sjöström (@pjsjostrom.bsky.social)

I am delighted to highlight our recent publication in The Innovation — @theinnovation.bsky.social — titled “Principles of visual cortex excitatory microcircuit organization”, where we showcase optomapping, ie 2p optogenetic circuit mapping! www.cell.com/the-innovati...

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Principles of visual cortex excitatory microcircuit organization Synapse-specific connectivity and dynamics determine microcircuit function but are challenging to explore with classic paired recordings due to their low throughput. We therefore implemented optomapping, a ∼100-fold faster two-photon optogenetic method. In mouse primary visual cortex (V1), we optomapped 30,454 candidate inputs to reveal 1,790 excitatory inputs to pyramidal, basket, and Martinotti cells. Across these cell types, log-normal distribution of synaptic efficacies emerged as a principle. https://www.cell.com/the-innovation/fulltext/S2666-6758(24)00173-5#author-highlights-abstract