Isolation and Co-culture of Paneth Cells and Intestinal Stem Cells Work by Ryosuke Isotani, Masaki Igarashi, Masaomi Miura and Toshimasa Yamauchi at The University of Tokyo. #LifeSciences #Reproducibility #StemCell

Practical Guide to In Vitro Clonal Propagation of Nicotiana benthamiana Using Axillary Shoot Induction Work by Pradeep Chand Deo at Plant Cell & Tissue Culture Professional. #LifeSciences #Reproducibility #PlantScience

A Protocol for Weighted Gene Co-expression Network Analysis With Module Preservation and Functional Enrichment Analysis for Tumor and Normal Transcriptomic Data: bio-protocol.org/en/bpdetail?...

📢Bio-protocol Webinars present: Optimizing live-cell imaging: From probe to FLIM-STED integration 🔗Register now! Date: October 15, 2025 ⏰ Time: 11:00 AM EST Speaker: Magalie Bénard, Ph.D. Moderator: Christophe Chamot This webinar is supported by Leica Microsystems. @leicamicrosystems.bsky.social

Bio-protocol's new issue is now live! 🎉 bio-protocol.org/en/archive?v...

NanoPDLIM2-Based Combination Therapy for Lung Cancer Treatment in Mouse Preclinical Studies Work by Thi Hoa Le et al. at University of Southern California Keck School of Medicine, and Fan Sun at University of Pittsburgh School of Medicine. #LifeSciences #Reproducibility #CancerBiology

Training Mice to Perform Attentional Set-Shifting Under Head Restraint Work by John J. Stout et al. at University of Connecticut School of Medicine, and Katarina Kalajzic at University of Connecticut. #LifeSciences #Reproducibility #Neuroscience

Quantitative Microscopy for Cell–Surface and Cell–Cell Interactions in Immunology Work by Beatriz Díaz-Bello, Dalia El Arawi, Rémy Torro, Patrick Chames, Kheya Sengupta and Laurent Limozin at Turing Center for Living Systems. #LifeSciences #Reproducibility #Immunology

Novel Experimental Approach to Investigate Immune Control of Vascular Function Work by Taylor C. Kress, Candee T. Barris, Simone Kennard and Eric J. Belin de Chantemèle at Medical College of Georgia at Augusta University. #LifeSciences #Reproducibility #Immunology

Simultaneous RNA Fluorescent In Situ Hybridization and Immunofluorescent Staining of Mouse Muscle Stem Cells on Fresh Frozen Skeletal Muscle Sections Work by Vedant R. Lakkundi et al. at University of Southern California. #LifeSciences #Reproducibility #StemCell

New Approach to Detect and Isolate Rhamnogalacturonan-II in Arabidopsis thaliana Seed Mucilage Work by Dayan Sanhueza and Susana Saez-Aguayo at Universidad Andrés Bello. #LifeSciences #Reproducibility #PlantScience

Ultrafast Isolation of Synaptic Terminals From Rat Brain for Cryo-Electron Tomography Analysis Work by Rong Sun and Qiangjun Zhou at Vanderbilt University. #LifeSciences #Reproducibility #Neuroscience

Real-Time Imaging of Specific Genomic Loci With CRISPR/dCas9 in Human Cells Using CRISPRainbow Work by Thomas J. Versosky, Dilshodbek U. Nishonov and Li-Chun Tu at Ohio State University. #LifeSciences #Reproducibility #Biophysics

Use of a High-Affinity Ubiquitin-Binding Domain to Detect and Purify Ubiquitinated Substrates and Their Interacting Proteins Work by Nitu Saha, Mengwen Zhang and Mark Hochstrasser at Yale University. #LifeSciences #Reproducibility #Biochemistry

Bio-protocol's new issue is now live! 🎉 bio-protocol.org/cn/archive?v... Check out the latest step-by-step protocols in various #LifeSciences fields. #Biochemistry #Biophysics #CancerBiology #Immunology #MolecularBiology #Neuroscience #PlantScience

Production of Homogeneous, Functional Zinc-Finger Arrays in High Yield With Two Chromatographic Steps Work by Jingchang Liang et al. at Stanford University, and Guanqiao Wang and Yan Nie at ShanghaiTech University. #LifeSciences #Reproducibility #Biochemistry

Bio-protocol's next webinar on Proteome Birthdating: A single-sample approach to measuring protein turnover and age, is just 1 day away! 🗓 Date: September 9, 2025 ⏰ Time: 11:00 AM EST Speaker: Michael Meadow, PhD Moderator: Emilie Besnard, PhD 🔗 Register now! #Webinar #LifeSciences

Rapid Isolation and Flow Cytometry Analysis of Murine Intestinal Immune Cells After Chemically Induced Colitis Work by Ashish K. Singh, Alfonso Blanco and Ulla G. Knaus at University College Dublin, and Ray Sinnott at Syntec International. #LifeSciences #Reproducibility #Immunology

Isolation and Ex Vivo Testing of CD8(+) T-Cell Division and Activation Using Mouse Splenocytes Work by Melissa Dolan, Yuhao Shi, Amber McKenery, et al. at Roswell Park Comprehensive Cancer Center. #LifeSciences #Reproducibility #CancerBiology

Assessing Human Treg Suppression at Single-Cell Resolution Using Mass Cytometry Work by Jonas Nørskov Søndergaard, Janyerkye Tulyeu, David Priest, Shimon Sakaguchi and James B. Wing at The University of Osaka. #LifeSciences #Reproducibility #Immunology

ClearDepth Method for Evaluations of Root Depth in Soil-Filled Pots Work by Michel Ruiz Rosquete, Juan Gonzalez, Natalie Gonzalez, Kristen Wertz, Suyash Patil and Wolfgang Busch at Salk Institute for Biological Studies. #LifeSciences #Reproducibility #PlantScience

Protocol for Quantifying γH2AX Foci in Irradiated Cells Using Immunofluorescence and Fiji Software Work by Lu Deng, Danning Wang and Lingying Wu at Chinese Academy of Medical Sciences and Peking Union Medical College. #LifeSciences #Reproducibility #CancerBiology

Intravitreal NHS-Biotin Injection and Immunohistochemistry to Label and Image Protein Transport in the Mouse Optic Nerve Work by Caroline R. McKeown, Lucio M. Schiapparelli, Hollis T. Cline at Neuroscience Department and Dorris Neuroscience Center. #LifeSciences #Reproducibility #Neuroscience

📢 Reminder: Don't miss our upcoming webinar, 'Proteome Birthdating: A single-sample approach to measuring protein turnover and age'. 🔗 Register now: s.bio-protocol.org/125b4cda7d7c...! 🗓 Date: September 9th, 2025 ⏰ Time: 11:00 AM EST Speaker: Michael Meadow, PhD #Webinar #LifeSciences

Heartiest congratulations to Dr. Hemant Prajapati, on his transition to Associate Editor at Bio-protocol! We’re delighted to see him take on this new role and continue contributing to advancing reproducibility in scientific research. #LifeSciences #Research #Reproducibility #OpenScience

Heartiest welcome to Dr. Marie Grandjean (@mmgrandjean.bsky.social‬), our new Assistant Editor at Bio-protocol! We’re delighted to have you join our editorial team and contribute to advancing reproducibility in scientific research. #LifeSciences #Research #Reproducibility #OpenScience

Heartiest welcome to Dr. Shengze Yao, our new Associate Editor at Bio-protocol! We’re delighted to have you join our editorial team and contribute to advancing reproducibility in scientific research. #LifeSciences #Research #Reproducibility #OpenScience

Heartiest welcome to Dr. Natalie M Doig, our new Assistant Editor at Bio-protocol! We’re delighted to have you join our editorial team and help to advance reproducibility in scientific research. #LifeSciences #Research #Reproducibility #OpenScience

An Optimized Enzyme-Coupled Spectrophotometric Method for Measuring Pyruvate Kinase Kinetics Work by Saurabh Upadhyay at Indian Institute of Technology. #LifeSciences #Reproducibility #Biochemistry

Integrated Membrane Yeast Two-Hybrid System for the Analysis of Membrane Protein Complexes Work by Brianna L. Greenwood, Kana Oshima and David T. Stuart at University of Alberta. #LifeSciences #Reproducibility #Microbiology

Assembly and Mutagenesis of Human Coronavirus OC43 Genomes in Yeast via Transformation-Associated Recombination Work by Brett A. Duguay and Craig McCormick at Dalhousie University. #LifeSciences #Reproducibility #Microbiology

Transplantation of Cultured Myoblasts Into Intact Skeletal Muscle and Analysis of Muscle Contraction Force in Mice Model Work by Kitora Dohi and Yasuro Furuichi at Tokyo Metropolitan University. #LifeSciences #Reproducibility #Cell Biology

Prokaryotic Expression and Purification of the hSox2-HMG Domain Work by Lijie Yang, Wansen Tan and Jingjun Hong at Anhui University. #LifeSciences #Reproducibility #Biochemistry

Bio-protocol's new issue is now live! 🎉 bio-protocol.org/en/archive?v... Check out the latest step-by-step protocols in various #LifeSciences fields. #Biochemistry #CancerBiology #CellBiology #Immunology #Microbiology #MolecularBiology #Neuroscience #PlantScience

📢 Bio-protocol Webinars present: Proteome Birthdating: A single-sample approach to measuring protein turnover and age 🔗 Register here: s.bio-protocol.org/125b4cda7d7c... Join us on: 🗓 Date: September 9th, 2025 ⏰ Time: 11:00 AM EST Speaker: Michael Meadow, Ph.D. #Webinar #LifeSciences #Proteomics

Heartiest welcome to Navnita Dutta, our new Associate Editor at Bio-protocol! We’re delighted to have you join our editorial team and help to advance reproducibility in scientific research. #LifeSciences #Research #Reproducibility #OpenScience

Heartiest welcome to Dr. Dana Savulescu (@danamanuela.bsky.social), our new Assistant Editor at Bio-protocol! We’re delighted to have you join our editorial team and help to advance reproducibility in scientific research. #LifeSciences #Research #Reproducibility #OpenScience

In the August 5 issue of Bio-protocol: two back-to-back protocols from the labs of Carolyn R. Bertozzi (2022 Nobel Laureate in Chemistry) and Tony Wyss-Coray at Stanford University.

Live Leaf-Section Imaging for Visualizing Intracellular Chloroplast Movement and Analyzing Cell–Cell Interactions Work by Yuta Kato, Takao Oi, Yoshikatsu Sato and Mitsutaka Taniguchi at Nagoya University. #LifeSciences #Reproducibility #PlantScience

Locomotor Activity Monitoring in Mice to Study the Phase Shift of Circadian Rhythms Using ClockLab (Actimetrics) Work by Andrea Brenna, Jürgen A. Ripperger and Urs Albrecht at University of Fribourg. #LifeSciences #Reproducibility #Neuroscience

Reprogramming White Fat Cells for Adipose Manipulation Transplantation (AMT) Therapy Work by Kelly An, Yusuke Ito and Nadav Ahituv at University of California San Francisco. #LifeSciences #Reproducibility #CellBiology

Heartiest welcome to Dr. Madhumala Sadanandappa, our new Associate Editor at Bio-protocol! We’re delighted to have you join our editorial team and help advance reproducibility in scientific research. #LifeSciences #Research #Reproducibility #OpenScience

On the cover ✨: This image shows adipocytes with lipid droplets highlighted by oil red staining. To learn more about the work from our latest issue, click on the full protocol link 👉 bio-protocol.org/en/bpdetail?... #Reproducibility #LifeSciences

A Step-By-Step Protocol for Correlative Light and Electron Microscopy Imaging of Proteinaceous Deposits in Cultured Cells and Human Brain Tissues Work by Peizhou Jiang and Dennis W. Dickson at Mayo Clinic. #LifeSciences #Reproducibility #Neuroscience

Heartiest welcome to Dr. Migla Miskinyte, our new Associate Editor at Bio-protocol, bringing expertise in bioinformatics and computational biology! We’re delighted to have you join our editorial team and help advance reproducibility in scientific research. #LifeSciences #Research #Reproducibility

Analyzing RNA Localization Using the RNA Proximity Labeling Method OINC-seq Work by Megan C. Pockalny, Hei-Yong G. Lo, Raeann Goering and J. Matthew Taliaferro at University of Colorado Anschutz Medical Campus. #LifeSciences #Reproducibility #BioinformaticsandComputationalBiology

Luminal Cerebrovascular Proteomics Work by Sophia M. Shi, Carolyn R. Bertozzi and Tony Wyss-Coray at Stanford University. #LifeSciences #Reproducibility #Neuroscience

Accurate Identification of Cell Cycle Stages in RPE1 Cells Using the ImmunoCellCycle-ID Method Work by Syon Reddy, Yu-Lin Chen and Aussie Suzuki at University of Wisconsin-Madison. #LifeSciences #Reproducibility #CellBiology

Direct Activity Measurement of Heterotrimeric Gi Proteins and Gq Protein By Effector Pulldown Work by Keiichiro Tanaka Jha and Martin A. Schwartz at Yale University. #LifeSciences #Reproducibility #Biochemistry

Bio-protocol's new issue is now live! 🎉 bio-protocol.org/en/archive?v... Check out the latest step-by-step protocols in various #LifeSciences fields. #Biochemistry #BioinformaticsandComputationalBiology #CellBiology #DevelopmentalBiology #Neuroscience #PlantScience #SystemsBiology