Starting to write up the first mechanistic paper from my lab about regulation of endosomal PI(3)P. We see retromer tubules (Vps35) budding from PI(3)P positive sorting endosomes with Actin at the neck. When PI(3)P homeostasis is perturbed this organisation breaks down and cells cant recycle cargo.

Nothing better for procrastination than going through some live cell imaging of Endosomes... Today we have the fusion of two equally sized PI(3)P positive endosomes. This is at 5s framerate, wish I had taken it at higher temporal resolution but still super cool to see

How do Endosomes dance? Looks like a waltz? Or maybe the more of a cèilidh? Cells expressing the PI(3)P probe imaged every 5s for ~15min. Currently preparing a really exciting paper about the regulation of endosomal PI(3)P homeostasis. Stay tuned!

Starting to put together an exciting paper about endosomal membrane identity and PowerPoint's AI knows exactly what I am up to: "A collage of different spots". Sums up this whole paper pretty well 😅

Great resource and evaluation on everything to do with preprints in the life sciences! Preprints have been transformational and there is a lot of good work being done at @ripplingideas.bsky.social !

Imaging phosphoinositides during mitosis ! Cells stained against PI(4,5)P2 using recombinant biosensors (tinyurl.com/447fabz8) PI(4,5)P2 is up regulated for cortex-membrane attachment. Also notice the membranes being shed and taken up by non-mitotic bystanders. #blebbisomes @mag2art.bsky.social?

We're hiring! Enjoyed @jackholcombe.bsky.social PhD Prize talk @edrc2025.bsky.social @fly-eds.bsky.social? We're recruiting a #postdoc to explore how cell biology is patterned in the #Drosophila renal system. Come join us for live-imaging, molecular cell biology and omics! Deadline 25th January.

Early bird deadline in 10 days!! This is going to be amazing!

Two days left to apply for a PhD position in plant development with me, Sam Amsbury and Andrew Fleming!!

While this does not solve the underlying issues with for-profit entities exploiting the free labour of academics, it does make my life easier in the short term. At the moment funders require me to publish open access while refusing to pay for the APC (a bit of a catch22). So this will help with that

#membrane seminars are back this THURSDAY for a super session with @dr-downes.bsky.social from the @giuliazanetti.bsky.social lab and Xin Zhou from the Ikonen lab! @felixmendu.bsky.social @ishier.bsky.social

This is making the rounds and people are be worried about fake data being published. I don’t see the point. All data can already be faked. All graphs and numerical data can freely be made up. Controls and samples can be switched. If we can trust scientists and institutions we might as well stop.

Really glad to show our recent work using recombinant phosphoinositide probes to investigate membrane identity changes during endosomal trafficking. Should be a great mini symposium to highlight the power of fluorescent microscopy techniques for cell biology!

📣Paper alert! Interested in faster and brighter protein labeling than before? 👀 @steffi-k.bsky.social & other colleagues have developed an improved tool for labeling proteins with synthetic fluorophores for bioimaging. Also works in STED microscopy 🤩🔬 Congratulations!🥳 www.nature.com/articles/s41...

Shit.

In all my interactions with @jacoates.bsky.social I can vouch for his sincere commitment and drive towards open science and engagement. Moving on from his role with ASAPbio will be a great opportunity for whomever he will work with next!

Choose Science. Choose Europe. A new Marie Skłodowska-Curie Actions Postdoctoral Fellowships 2025 call is now open. With a budget of €404.3 million, it will support around 1,650 researchers from Europe and beyond. Apply by 10 September → europa.eu/!fBTMgF

Looking for a student or postdoc! Want to combine evolutionary analysis + structural modeling with in vitro biochemistry & fluorescence microscopy? Help us uncover new protein-protein interactions — from in silico to in vitro. Join us at @istaresearch.bsky.social 👉 looselab.org 🧬💡🔬🎡

If using Bloomigton #Drosophila Stock Center stocks, pls. acknowledge them & their NIH funding (P40 OD018537). Papers listing this no. are being harvested as evidence. We massively depend on the @bdsc.bsky.social & they need our support in these dire times! @flybase.bsky.social @fly-eds.bsky.social

Pls re-skeet! There's a postdoc job available to join us in Bristol! We're looking for a molecular cell biologist to help us investigate plasma membrane proteostasis in neurons. Apply here by 23 April: www.jobs.ac.uk/job/DMO097/r.... If interested, do contact me and check out grieve-lab.com! Thanks!

Only 10 days left to apply! We are looking for a passionate protein biochemist for the reconstitution of lipid kinases. We have long term funding and you will be trained in the use of supported lipid bilayers. If you are interested just get in touch or apply directly!

First time playing around with single molecule STORM imaging of PI3P on endosomes using recombinant biosensors. This was great fun with our new fellow Ruby Peters and I see lots of potential for this in the future!

🚨Long term postdoc position available on the reconstitution of lipid kinases!🚨 Do you have a passion for protein reconstitution and lipid signalling? You will reconstitute lipid kinases from eukaryotic expression systems to determine their kinetic regulation. www.jobs.ac.uk/job/DLW981/r...

The open science community must lead by example in defending science. We are ideally positioned to combat the efforts by Trump, Musk & others that are anti-science & anti-inclusion. Time to practice what we preach! #academicsky #openscience #metasci #ScholComm

Registration is open for the 2025 iteration of the wonderful @EMBOevents workshop on cell polarity and membrane dynamics #EMBOcellPolarity25 I am organizing with Ed Munro! Wonderful lineup wonderful please please sign up and share! meetings.embo.org/event/25-cel...

Incredibly honoured to have my first paper as PI highlighted in this collection! The idea was do a quick tool paper as my start into independence and it turned out to be much more useful than I thought. Read about how to use recombinant biosensors to stain for lipids in fixed cells!

I want to share one of my favourite #microscopy images with you all: Epithelial cells in a monolayer (NMuMG) after undergoing EMT stained for N-Cadherin and actin show beautifully interdigitated cell junctions. Bonus points for spotting the actin asters ⭐

Clathrin-coated pits, plaques, and adhesion... This post is dedicated to Christien Merrifield, who left us far too soon around this time in 2017. His memory continues to inspire 🔬 doi.org/10.1016/j.js...

*Last few days to apply!* We're looking for a #DevBio researcher interested in gaining #SciComm experience to join us at @biologists.bsky.social Biologists @ 100 conference. We're open to any ideas💡If selected, we'll work with you to develop your ideas and assist you at the conference. More info👇

#Preprints allowed @maiblab.bsky.social ✍️ disseminate research to the community 🗯️ get valuable information about how well it is received 💡 highlight to journals that there is a strong interest in Hannes' work 📄 made it easier to get past the editor's desk

Trying this out for #fluorescencefriday. 🧪 AktPH through an isosurface mesh of the plasma membrane. From this lattice lightsheet collaboration with @minesbioimaging.bsky.social: www.nature.com/articles/s41...

Congratulations to Meiyan Jin on her beautiful publication out today. www.sciencedirect.com/science/arti...

Great opportunity for postdocs interested in molecular neuroscience/ biochemistry to study presynapse formation in human neurons funded by the ERC (see www.leibniz-fmp.de/haucke; Rizalar et al Haucke (2023) Science). We are a certified green lab. Apply now to [email protected] before 5 Dec 2024.

On my way to brave the snow to get to the EndoConnect conference (endoconnect.eu/endoconnect-...) in Cambridge now. Really looking forward to three days of endo/lysosomal sorting.

In my first real post I want to show the key experiment that revealed to me what I want my own lab to be about: The conversion of phosphoinositides! By using fluorescent biosensors and membrane coated beads I was able to reconstitute the real time kinetics of PI(4)P to PI(4,5)P2 conversion by PIP5K

New to this place. How do I share all the beautiful GIFs of my cells and membrane reconstitution? It looks like it should be able to do it in the preview but then just posts still images of the first frame!