Faster, clearer, deeper 3D imaging Researchers developed an innovative light sheet fluorescence microscopy platform, which means detailed scans of fine networks of nerves or blood vessels are possible faster: www.uni-goettingen.de/en/3240.html... #NatureBiotechnology: doi.org/10.1038/s415...

Lichtblattmikroskope erstellen 3D-Scans von Geweben und Organen – erzeugen bei großen Proben aber unscharfe Bilder. Forschende haben eine Plattform entwickelt, die diese Hürde überwindet. Mehr erfahren: www.uni-goettingen.de/de/3240.html... #MBexC #UMG @mbexc.bsky.social @huiskenlab.bsky.social

Absolutely loved this paper. Really addresses my personal gripes of not having isotropic voxel size in microscopy imaging rdcu.be/eRgYm

Preprint alert! CochleaNet, our framework for analyzing light-sheet data of the cochlea. It consists of three networks to segment spiral ganglion neurons, inner hair cells, and to detect synapses. See rendering of a full cochlea in the image, find the preprint at doi.org/10.1101/2025....

Studying cochlear neuroanatomy demands imaging the entire cochlea at subcellular resolution. In our recent study (tinyurl.com/isotropicLSFM), we recorded the cochlea at isotropic resolution across the whole organ, laying the groundwork for what follows… #lightsheet #isotropic #cochlea

For technical details see the following post:

Very happy to share our latest work extending iterative immunofluorescence to in toto imaging of early zebrafish embryos (3D-4i), integrated with a 3D-dedicated image analysis pipeline! 🐟🔬📊 Huge kudos to Max Hess for this PhD milestone! 💪 @lucaspelkmans.bsky.social shorturl.at/cO0u7 Let's zoom in!

I am happy to share a recent collaboration with @huiskenlab.bsky.social and @aakhte.bsky.social , who developed a great technique for performing fast and, above all, isotropic light-sheet microscopy. www.nature.com/articles/s41...

🎉 I am very glad to share that our paper on the rapid isotropic light-sheet microscope has just been published in Nature Biotechnology @natbiotech.nature.com. 🔗 Read the full article: www.nature.com/articles/s41...

Happy to see our paper “Isotropic, aberration-corrected light sheet microscopy for rapid high-resolution imaging of cleared tissue” in @natbiotech.nature.com – with Tobias Moser’s lab from @unimedizin-goe.bsky.social and @janwenzel.bsky.social www.nature.com/articles/s41... #microscopy #lightsheet

Research Briefing: High-speed isotropic light-sheet microscopy www.nature.com/articles/s41... rdcu.be/ePWjG

Isotropic, aberration-corrected light sheet microscopy for rapid high-resolution imaging of cleared tissue - @huiskenlab.bsky.social go.nature.com/4odcS49

Hybrid Solid-Liquid Optics Enable Scalable, High-Resolution, Multi-Immersion Light-Sheet Microscopy www.biorxiv.org/content/10.1...

Our image of a sea squirt oozoid (Thalia democratica) was selected as an Image of Distinction in Nikon Small World. Imaged with the Flamingo microscope by @metteh-thorsager.bsky.social at @biodev-vlfr.bsky.social in Villefranche-sur-Mer. Salp sample courtesy of Alexandre Alie. nikonsmallworld.com/

The future of High-Content Imaging has arrived. -> Ultra-fast 3D imaging of multi-well plates at high resolution using an air objective! See previous posts and the new miOPM preprint for details: doi.org/10.1101/2025...

Huisken lab is attending Göttingen Cognition Forum: Curiosity & Interaction. Learn about our research... @spp2205.bsky.social @sfb1528.bsky.social @rtg2906-curiosity.bsky.social @uni-goettingen.de events.gwdg.de/event/1099/

Postdoc opportunity in the Averof lab: a crustacean meets a flamingo—not on the menu, but in the light sheet. Track the origins of cells during leg regeneration in Parhyale hawaiensis using light sheet microscopy. Excited to be part of this interdisciplinary project. Details on how to apply below.

We present a simple method to easily increase the imageable depth of an expansion microscopy gel on a typical inverted microscope ten-fold, using some carefully placed FEP film and a water dipping objective lens:

The Huisken lab is now on Bluesky @huiskenlab.bsky.social - follow us for updates on our activities!

We brought our Flamingo microscope to the light sheet microscope course in Dresden (#LISH25). We captured extensive time-lapse recordings and are excited to share highlights from our experiments with Hydra and Zebrafish.

#biabob 0.28.0 is out and it comes with support for OpenAI's codex-mini model. I also enabled its vision-support. With this, you can turn screenshots of user-interfaces into functional code - directly within your Jupyter notebook 🤯 github.com/haesleinhuep...

We are excited to share our development of a new 10X lens with NA=0.50 over a 7.2 mm FOV and a 35 mm working distance (water), fully corrected from 400-850 nm. This lens will power our upcoming ExA-SPIM "2" and is available for dissemination - please get in touch if interested!

Our manuscript on accelerating the acquisition rate in oblique plane microscopy (OPM) is online now: opg.optica.org/boe/fulltext... Our work addresses the issue that an OPM PSF is tilted, and as such it leaves a lot of "empty space" in Fourier space (FFT of experimental data on the right)... 1/n

Thermal Lensing Effects in Light-Sheet Microscopy arxiv.org/abs/2504.02969

Microscope objectives can be confusing, and detailed specs (or even basic properties) are often hard to find. Here's an introductory resource to help new builders and instrument designers with the basics. It's a work in progress so feedback is appreciated: amsikking.github.io/microscope_o...

To solve the collision problem in OPM (previous post), lower numerical aperture (NA) objectives with longer working distances were used: -> Here we show the pupils in a remote refocus (3 circles). The reduced NA in the downstream objectives loses light (yellow circle) and lowers the resolution!

Remote refocus (RR) strikes again! ;) Congrats to @aakhte.bsky.social and team on their interesting blend of opto-mechanical innovations!

Isotropic, aberration-corrected light sheet microscopy for rapid high-resolution imaging of cleared tissue www.biorxiv.org/content/10.1...

Impressive & clever. A light-sheet microscope with isotropic resolution <1 μm. Uses a using a concave mirror to correct optical abberation and swipes the light sheet. Validated on mouse brain vasculature & cochlea . @aakhte.bsky.social Huisken lab www.biorxiv.org/content/10.1...

Our first deposition of synaptic vesicles segmentations is now in the Cryo ET Portal! We segmented vesicles in over 50 tomograms to enable analysis of membrane proteins and more. cryoetdataportal.czscience.com/depositions/...

After a long journey, Segment Anything for Microscopy is now published in Nature Methods! We significantly improve SAM for interactive and automatic segmentation in light and electron microscopy and build a user-friendly tool. www.nature.com/articles/s41...