Close-up of vesicular ER exit sites by volume electron imaging using FIB-SEM. New report from Athul Nair, Tom Kirchhausen @kirchhausenlab.bsky.social and colleagues @bostonchildrens.bsky.social: rupress.org/jcb/article/... #Trafficking #Technology #ER_literature

#Tubulin regulates stability and localization of STMN2 by binding preferentially to its soluble form, say Xiang Deng, Timothy Mitchison and colleagues @harvardmed.bsky.social: rupress.org/jcb/article/... #ProteinHomeostasis #Neuroscience #Biochemistry #Cytoskeleton #Neurodegeneration #ALS

Calvo, Patel, Alonso @uva-es.bsky.social et al. describe a new bioluminescent sensor for measuring luminal Ca²⁺ in a mildly acidic subcompartment of the endo-lysosomal system. rupress.org/jcb/article/... #CellSignaling #Organelles #Physiology #Technology #Lysosomes #Endosomes

PCMD-1 stabilizes the PCM scaffold and facilitates #centriole separation, say Alina Schreiner, Tamara Mikeladze-Dvali @tdvalilab.bsky.social and colleagues: rupress.org/jcb/article/... @zaninlab.bsky.social #Centrosome #PCMstability #Celegans #Development #CellDivision

Roles of Srs2/PARI-family DNA helicases in NoCut checkpoint signaling and abscission regulation. New study from Monica Dam, David F. Moreno, Nicola Brownlow, Manuel Mendoza (@longchrom.bsky.social) and colleagues @igbmc.bsky.social: rupress.org/jcb/article/... #Genetics #CellDivision #Cytokinesis

Our paper on PCMD-1 and centrosome stability is finally online! Congratulations 🎉🎉🎉 to Alina and the entire team! Very proud and grateful - it was an incredible teamwork @zaninlab.bsky.social! @lifescimunich.bsky.social. If you want to read more check it out on JCB! rupress.org/jcb/article-...

Just published! We share an open-source workflow to measure membrane thickness from tomograms, including a tutorial with 3D visualizations. We analyze thickness variations across organelles and reflect on where to define a membrane boundary. @becklab.bsky.social #teamtomo rupress.org/jcb/article/...

Okada, Bi et al. @upenn.edu show that Bni5 interacts with #septin filaments, septin-associated kinase, and #myosin-II to regulate septin hourglass architecture and remodeling, retrograde #actin flow, and #cytokinesis rupress.org/jcb/article/... #Biochemistry #CellDivision #Cytoskeleton #Genetics

Peiyao Fan, Yang Chen et al. @pku1898.bsky.social show that tubular ER extends into retraction fibers and #migrasomes through #microtubule-dependent ER extension and ER-plasma membrane contact sites in migrating cells. rupress.org/jcb/article/... #ER_literature #Migration #Organelles

Great work of @deglushk.bsky.social from my PhD lab @mpibp.bsky.social on how to get more information out of all the membranes you see in cryoET data!

Very thorough work that applies cryo-ET to measure bilayer thickness. Surprisingly, the missing wedge is not a big problem!

Irg1l regulates neuromast size via metabolic reprogramming to promote supporting cell proliferation. New study from Xin Wang, Ruijun Shi, Yuqing Xiang, Yajing Gao, Guoqiang Wan, Shan Sun, and Dong Liu (Nantong University): rupress.org/jcb/article/... #CellMetabolism #Development #Zebrafish

In new study from Ryken, Bezanilla et al., analysis of PIIB-type calcium pump localization and function elucidates one of the molecular mechanisms that maintains the strength of the calcium gradient during polarized growth. rupress.org/jcb/article/... #PlantBiology #Cytoskeleton #Trafficking

Xu Liu, Ya Huang et al. show that the transcriptional regulator RTF1 promotes #circadian rhythm by enhancing CLOCK occupancy and H3K4me3 deposition at pacemaker gene loci. rupress.org/jcb/article/... #Chromatin #Epigenetics

Glushkova, Böhm, & Beck @maxplanck.de develop a publicly available computational method to measure the thickness of biological membranes in cryo-electron tomograms. Analysis of algae & human cells reveals systematic membrane thickness variations within & across organelles rupress.org/jcb/article/...

Delighted to share the latest chapter in the mystery series on how septins sense membrane curvature. Grateful to reviewers for thoughtful critiques and @jcb.org for publishing our work! doi.org/10.1083/jcb....

Moesin strikes an "Actin"g G-balance to regulate #osteoclast fusion and activity. Marwa Zeyad and Yousef Abu-Amer @washumedicine.bsky.social highlight new work from the @chrisverollet.bsky.social group (rupress.org/jcb/article/...) in Spotlight: rupress.org/jcb/article/... #CellSignaling

Spotlight: Jianuo Han and Yansong Miao highlight recent findings from Magliozzi et al. (rupress.org/jcb/article/...), identifying a novel “composite” #actin nucleator for the assembly of thick actin cables. rupress.org/jcb/article/... #Biochemistry #Cytoskeleton

Xinyang Li, Yue Hua et al. demonstrate that B4GALT1 and WLS cooperate to retain the canonical #Wnt receptor LRP5/6 on the #Golgi apparatus, leading to specific inhibition of the Wnt/β-catenin signaling in such Wnt-receiving cells that have no expression of Wnt proteins. rupress.org/jcb/article/...

Localization of the #septin #cytoskeleton is controlled by regulatory factors, membrane curvature, & charge. In this study, @gladfelterlab.bsky.social finds changes to #lipid composition that modulate lipid packing defects to impact septin assemblies in vitro & in cells. rupress.org/jcb/article/...

JCB’s November issue is here! rupress.org/jcb/issue/22... Cover shows a scanning electron #microscopy image of a human osteoclast derived from blood monocytes on bone slices. Image © Dufrançais and Poincloux. See doi.org/10.1083/jcb.... @chrisverollet.bsky.social

Moesin controls cell–cell fusion and #osteoclast function, say Ophélie Dufrançais, Brigitte Raynaud-Messina, Christel Vérollet (@chrisverollet.bsky.social) and colleagues @ipbstoulouse.bsky.social: rupress.org/jcb/article/... #Physiology #Cytoskeleton #Actin

Our story on Srs2/PARI family DNA helicases linking chromatin bridges to the Aurora-B dependent NoCut checkpoint is now out in its peer-reviewed version. Thanks to @reviewcommons.org and @jcb.org for a smooth review process! Original preprint thread here: bsky.app/profile/long...

Deng, Mitchison et al. @harvardmed.bsky.social employ two human cell models to elucidate how #tubulin binding, membrane association, and protein stability converge in the biology of STMN2, a protein implicated in amyotrophic lateral sclerosis. rupress.org/jcb/article/... #Neuroscience #ALS

What do ER exit sites really look like? Using large-scale volume FIB-SEM and a custom 3D-Unet pipeline (ASEM), Nahir et al @kirchhausenlab.bsky.social reveal that ERES are vesicular, abundant & diverse, resolving long-standing morphological discrepancies rupress.org/jcb/article/... #ER_literature

Schreiner, Mikeladze-Dvali et al. @lifescimunich.bsky.social show that CDK-1 primes PCMD-1 for PLK-1 phosphorylation. Mutations in PLK-1 docking sites impair SPD-5 binding, destabilize the PCM, and delay #centriole separation. rupress.org/jcb/article/... #Centrosome #PCMstability #Celegans

Dam, Moreno, Brownlow, @longchrom.bsky.social et al. @igbmc.bsky.social identify Srs2 as a key mediator of abscission inhibition in yeast and show that its human paralog PARI regulates abscission-associated events. rupress.org/jcb/article/... #Cytokinesis

TMEM175 does not function as a proton-selective ion channel to prevent lysosomal over-acidification, say Erika Riederer, Dejian Ren @upenn.edu and colleagues: rupress.org/jcb/article/... #CellSignaling #Physiology

#Fibroblasts promote osmotic surveillance by wound-induced unique calcium patterns. New study from László Fazekas, Diána Kaszás, Boldizsár Vámosi, Balázs Enyedi @enyedilab.bsky.social et al. (Semmelweis University): rupress.org/jcb/article/... #Physiology #Biochemistry #CellSignaling #Zebrafish

IQSEC2/BRAG1 may modulate postsynaptic density assembly through Ca2⁺-induced phase separation, say Guanhua Bai, Hao Li, Mingjie Zhang and colleagues (Southern University of Science and Technology, Shenzhen) rupress.org/jcb/article/... #Biochemistry #Neuroscience #CellSignaling

Phosphoproteomic identification of Mos-MAPK targets in meiotic cell cycle and asymmetric #oocyte divisions. New study from Ivan Avilov, Peter Lenart (@plenart.bsky.social) and colleagues @maxplanck.de: rupress.org/jcb/article/... #Cytoskeleton #CellCycle #CellDivision #Physiology #Meiosis

Beyond Selection: How chromosome 12 gain dominates stem cell genomes. Orléna Benamozig and Ofer Shoshani @weizmanninstitute.bsky.social highlight new work from @gorbskylab.bsky.social (rupress.org/jcb/article/...) in Spotlight: rupress.org/jcb/article/... #Aneuploidy #StemCells

Mechanical coordination between anaphase A and B drives asymmetric chromosome segregation, say Ana Dias Maia Henriques, Julien Dumont, Gilliane Maton and colleagues @ijmonod.bsky.social: rupress.org/jcb/article/... #Development #CellCycle #CellDivision #Celegans #Mitosis

Xin Wang et al. identify Irg1l as a regulator of #zebrafish neuromast size. They demonstrate that the Irg1l/itaconate axis promotes supporting cell proliferation by inducing metabolic reprogramming that activates Yap signaling rupress.org/jcb/article/... #CellMetabolism #Development

✍️ The Dumont Lab published a new article with contributions from the @minclab.bsky.social Lab 📖 @jcb.org 📃 Mechanical coordination between anaphase A and B drives asymmetric chromosome segregation 🔗 rupress.org/jcb/article-...

Proud that our work is published in @jcb.org. Thanks 🙏 for the great revision and publication process.

Too much cell fusion can break your bones, literally! IPBS researchers @chrisverollet.bsky.social & coll. found that moesin keeps bone-resorbing cells (osteoclasts) in check. Without it, bones weaken, a discovery that could inspire new #osteoporosis treatments. 📘 @jcb.org shorturl.at/N0Esc

Spotlight: Zeyad and Abu-Amer @washumedicine.bsky.social highlight work from Dufrancais @chrisverollet.bsky.social et al. (rupress.org/jcb/article/...) revealing a role of tunneling nanotubes in moesin-mediated #osteoclast fusion and regulation of #osteoclastogenesis. rupress.org/jcb/article/...

Dufrançais, Raynaud-Messina, @chrisverollet.bsky.social et al. show that moesin, a member of the ERM proteins, plays a negative regulatory role in #osteoclastogenesis. rupress.org/jcb/article/... #Physiology #Cytoskeleton #Actin

This is the first time I get credited in an article for... writing a plotting script. Thanks to the main authors ! We also had very interesting scientific discussions along the way, but that might come in an follow-up one day :)

A recent model suggests the #Parkinsons risk factor protein TMEM175 is primarily a proton-conducting channel. Riederer et al. @upenn.edu demonstrate that TMEM175 does not mediate a lysosomal proton leak and is not responsible for preventing lysosomal over-acidification. rupress.org/jcb/article/...

CD47 inhibits #phagocytosis through Vav dephosphorylation, say Wyatt Miller, Meghan Morrissey (@mmorrissey.bsky.social) and colleagues @ucsantabarbara.bsky.social: rupress.org/jcb/article/... #Immunology #CellSignaling

A key role for Canoe's intrinsically disordered region in linking cell junctions to the #cytoskeleton. New study from Corbin Jensen, Mark Peifer (@peiferlabunc.bsky.social) and colleagues @unclineberger.bsky.social: rupress.org/jcb/article/... #Development #Adhesion #Drosophila

Understanding #microtubule dynamics: The synergy of technology, theory, and experiment. New Perspective from Richard McIntosh @colorado.edu: rupress.org/jcb/article/... #Cytoskeleton #Biophysics #Microtubules

So pleased to see this out at JCB. Beautiful calcium imaging here… yet again demonstrating the power of zebrafish for exquisite imaging in vivo.

Dias Maia Henriques, Dumont, Maton et al. @ijmonod.bsky.social uncover an #anaphase A pathway, driven by the #kinesin-13 KLP-7 and opposed by the kinesin-12 KLP-18, that contributes to chromosome segregation in early #Celegans embryos. rupress.org/jcb/article/... #Mitosis #CellDivision #Spindle

Spotlight: Benamozig and Shoshani @weizmanninstitute.bsky.social highlight work from the Gorbsky Lab @gorbskylab.bsky.social (rupress.org/jcb/article/...), which shows how chromosomal instability can rapidly cause #aneuploidy. rupress.org/jcb/article/... #StemCells

Avilov, @plenart.bsky.social et al. @maxplanck.de used quantitative phosphoproteomics in #starfish #oocytes to identify downstream targets of the conserved Mos–MAPK pathway. rupress.org/jcb/article/... #Cytoskeleton #CellCycle #CellDivision #Physiology

New work from Guanhua Bai, Hao Li, Mingjie Zhang and colleagues reveals an unexpected function of IQSEC2 as a scaffold protein and thus explains why IQSEC2 can bidirectionally regulate synaptic strengths. rupress.org/jcb/article/... #Biochemistry #Neuroscience #CellSignaling

Fazekas, Kaszás, Vámosi et al @enyedilab.bsky.social describe a #fibroblast-specific synthetic promoter that enables #biosensor expression & targeted ablation in #zebrafish fins & reveal a role for fibroblasts in osmotic surveillance by wound-induced calcium patterns rupress.org/jcb/article/...