Quick assembly. Just don’t lose the Allen/Hex key.

Just a reminder, “Epstein” is the name of the sex trafficker. The president is called “Epstein’s monster.”

1/ A deep, Friday night dive into how we check the accuracy of SARS-CoV-2 wastewater signals. It's becoming more important with new variants in the past year. TL;DR: Mutations can affect PCR targets, so QC is important — but wastewater trends remain reliable and closely monitored for accuracy.

Avg. of SARS-CoV-2 N1, N2 or other viral genetic markers normalized to Pepper Mild Mottle Virus as a fecal strength indicator; Samples collected by @ottawacity.bsky.social, tested & analyzed by Delatolla lab (uOttawa); Data i/o by @dougmanuel.bsky.social; Plots and tweet bot by @rnaguru.bsky.social

Ottawa WAVE (Wastewater Analytics for Viral Epidemiology) as of Nov 16. LEFT IMAGE: 7d trendline of SARS-CoV-2 signal since spring 2020 (A); Past year (B), and 2 months (C), inferred case incidence (D), and Reff (E) determined with ern (PMID: 38905266). RIGHT IMAGE: other viral traces

Avg. of SARS-CoV-2 N1, N2 or other viral genetic markers normalized to Pepper Mild Mottle Virus as a fecal strength indicator; Samples collected by @ottawacity.bsky.social, tested & analyzed by Delatolla lab (uOttawa); Data i/o by @dougmanuel.bsky.social; Plots and tweet bot by @rnaguru.bsky.social

Ottawa WAVE (Wastewater Analytics for Viral Epidemiology) as of Nov 16. LEFT IMAGE: 7d trendline of SARS-CoV-2 signal since spring 2020 (A); Past year (B), and 2 months (C), inferred case incidence (D), and Reff (E) determined with ern (PMID: 38905266). RIGHT IMAGE: other viral traces

"[History] is really complicated, and you have to tell that complication. ... Any idea of boiling it down to simplistic slogans is not American," Ken Burns said. https://to.pbs.org/4p6Y47H

This is a very sad day for public health and evidence-based science in general.

Avg. of SARS-CoV-2 N1, N2 or other viral genetic markers normalized to Pepper Mild Mottle Virus as a fecal strength indicator; Samples collected by @ottawacity.bsky.social, tested & analyzed by Delatolla lab (uOttawa); Data i/o by @dougmanuel.bsky.social; Plots and tweet bot by @rnaguru.bsky.social

Ottawa WAVE (Wastewater Analytics for Viral Epidemiology) as of Nov 16. LEFT IMAGE: 7d trendline of SARS-CoV-2 signal since spring 2020 (A); Past year (B), and 2 months (C), inferred case incidence (D), and Reff (E) determined with ern (PMID: 38905266). RIGHT IMAGE: other viral traces

Cost per km is now pretty much the same as Montreal REM, arguably a more future-proofed system. www.cbc.ca/news/canada/...

Avg. of SARS-CoV-2 N1, N2 or other viral genetic markers normalized to Pepper Mild Mottle Virus as a fecal strength indicator; Samples collected by @ottawacity.bsky.social, tested & analyzed by Delatolla lab (uOttawa); Data i/o by @dougmanuel.bsky.social; Plots and tweet bot by @rnaguru.bsky.social

Ottawa WAVE (Wastewater Analytics for Viral Epidemiology) as of Nov 09. LEFT IMAGE: 7d trendline of SARS-CoV-2 signal since spring 2020 (A); Past year (B), and 2 months (C), inferred case incidence (D), and Reff (E) determined with ern (PMID: 38905266). RIGHT IMAGE: other viral traces

US government reopens. Finally, back to the important work of arguing over a pedo’s inbox. Truly productive.

King Gustaf is arriving in #YOW Tuesday. IKEA Ottawa is serving free meatballs all day to celebrate, right? www.gg.ca/en/media/new...

Avg. of SARS-CoV-2 N1, N2 or other viral genetic markers normalized to Pepper Mild Mottle Virus as a fecal strength indicator; Samples collected by @ottawacity.bsky.social, tested & analyzed by Delatolla lab (uOttawa); Data i/o by @dougmanuel.bsky.social; Plots and tweet bot by @rnaguru.bsky.social

Ottawa WAVE (Wastewater Analytics for Viral Epidemiology) as of Nov 09. LEFT IMAGE: 7d trendline of SARS-CoV-2 signal since spring 2020 (A); Past year (B), and 2 months (C), inferred case incidence (D), and Reff (E) determined with ern (PMID: 38905266). RIGHT IMAGE: other viral traces

Toad was lying in bed. He had pulled all the covers over his head.

True comedy is watching Dave Portnay trying to use a hammer

The beautiful game is spectacular in the snow. www.cbc.ca/sports/socce...

Avg. of SARS-CoV-2 N1, N2 or other viral genetic markers normalized to Pepper Mild Mottle Virus as a fecal strength indicator; Samples collected by @ottawacity.bsky.social, tested & analyzed by Delatolla lab (uOttawa); Data i/o by @dougmanuel.bsky.social; Plots and tweet bot by @rnaguru.bsky.social

Ottawa WAVE (Wastewater Analytics for Viral Epidemiology) as of Nov 09. LEFT IMAGE: 7d trendline of SARS-CoV-2 signal since spring 2020 (A); Past year (B), and 2 months (C), inferred case incidence (D), and Reff (E) determined with ern (PMID: 38905266). RIGHT IMAGE: other viral traces

Avg. of SARS-CoV-2 N1, N2 or other viral genetic markers normalized to Pepper Mild Mottle Virus as a fecal strength indicator; Samples collected by @ottawacity.bsky.social, tested & analyzed by Delatolla lab (uOttawa); Data i/o by @dougmanuel.bsky.social; Plots and tweet bot by @rnaguru.bsky.social

Ottawa WAVE (Wastewater Analytics for Viral Epidemiology) as of Nov 09. LEFT IMAGE: 7d trendline of SARS-CoV-2 signal since spring 2020 (A); Past year (B), and 2 months (C), inferred case incidence (D), and Reff (E) determined with ern (PMID: 38905266). RIGHT IMAGE: other viral traces

Avg. of SARS-CoV-2 N1, N2 or other viral genetic markers normalized to Pepper Mild Mottle Virus as a fecal strength indicator; Samples collected by @ottawacity.bsky.social, tested & analyzed by Delatolla lab (uOttawa); Data i/o by @dougmanuel.bsky.social; Plots and tweet bot by @rnaguru.bsky.social

Ottawa WAVE (Wastewater Analytics for Viral Epidemiology) as of Nov 02. LEFT IMAGE: 7d trendline of SARS-CoV-2 signal since spring 2020 (A); Past year (B), and 2 months (C), inferred case incidence (D), and Reff (E) determined with ern (PMID: 38905266). RIGHT IMAGE: other viral traces

To you from failing hands we throw The torch; be yours to hold it high. If ye break faith with us who die We shall not sleep, though poppies grow

NEW: 4 years ago, the government studied whether or not a tunnel under Highway 401 was doable, finding a risk of collapse and eventually pausing the work. Now, it is refusing to release the study - after paying a company $9.1M to do it again. #Onpoli globalnews.ca/news/1151718...

Avg. of SARS-CoV-2 N1, N2 or other viral genetic markers normalized to Pepper Mild Mottle Virus as a fecal strength indicator; Samples collected by @ottawacity.bsky.social, tested & analyzed by Delatolla lab (uOttawa); Data i/o by @dougmanuel.bsky.social; Plots and tweet bot by @rnaguru.bsky.social

Ottawa WAVE (Wastewater Analytics for Viral Epidemiology) as of Nov 02. LEFT IMAGE: 7d trendline of SARS-CoV-2 signal since spring 2020 (A); Past year (B), and 2 months (C), inferred case incidence (D), and Reff (E) determined with ern (PMID: 38905266). RIGHT IMAGE: other viral traces

nobody needs a billion $$.

Put it in the Smithsonian!

Avg. of SARS-CoV-2 N1, N2 or other viral genetic markers normalized to Pepper Mild Mottle Virus as a fecal strength indicator; Samples collected by @ottawacity.bsky.social, tested & analyzed by Delatolla lab (uOttawa); Data i/o by @dougmanuel.bsky.social; Plots and tweet bot by @rnaguru.bsky.social

Ottawa WAVE (Wastewater Analytics for Viral Epidemiology) as of Nov 02. LEFT IMAGE: 7d trendline of SARS-CoV-2 signal since spring 2020 (A); Past year (B), and 2 months (C), inferred case incidence (D), and Reff (E) determined with ern (PMID: 38905266). RIGHT IMAGE: other viral traces

A wind came. It blew across the land.

How much are MPs entitled to know about research grants? Not as much as they think. A parliamentary committee is asking for researchers' personal information in what seems like an anti-DEI crusade, by @picardonhealth.bsky.social www.theglobeandmail.com/opinion/arti... via @theglobeandmail.com

Budget 2025 Easter egg: “The government…is working with CBC/Radio Canada to explore participation in Eurovision.”

Avg. of SARS-CoV-2 N1, N2 or other viral genetic markers normalized to Pepper Mild Mottle Virus as a fecal strength indicator; Samples collected by @ottawacity.bsky.social, tested & analyzed by Delatolla lab (uOttawa); Data i/o by @dougmanuel.bsky.social; Plots and tweet bot by @rnaguru.bsky.social

Ottawa WAVE (Wastewater Analytics for Viral Epidemiology) as of Oct 26. LEFT IMAGE: 7d trendline of SARS-CoV-2 signal since spring 2020 (A); Past year (B), and 2 months (C), inferred case incidence (D), and Reff (E) determined with ern (PMID: 38905266). RIGHT IMAGE: other viral traces

“I hope that if I bang my head against the wall, it will help me to think of a story,” said Toad.

“Furin cleavage site”, that is. Just casually, and naturally, sitting there in one of the most evolutionarily active parts of the genome. Mystery solved.

Some charts for those who don't know the extent of the US public (and private) investment in research (particularly in biomedical research) compared to other countries and institutions. The destruction of the US' scientific institutions has global implications.

“It’s an avalanche!” cried Toad. Frog and Toad jumped away. Frog was trembling. “I am not afraid!” he shouted.

Avg. of SARS-CoV-2 N1, N2 or other viral genetic markers normalized to Pepper Mild Mottle Virus as a fecal strength indicator; Samples collected by @ottawacity.bsky.social, tested & analyzed by Delatolla lab (uOttawa); Data i/o by @dougmanuel.bsky.social; Plots and tweet bot by @rnaguru.bsky.social

Ottawa WAVE (Wastewater Analytics for Viral Epidemiology) as of Oct 26. LEFT IMAGE: 7d trendline of SARS-CoV-2 signal since spring 2020 (A); Past year (B), and 2 months (C), inferred case incidence (D), and Reff (E) determined with ern (PMID: 38905266). RIGHT IMAGE: other viral traces

Google has a Halloween themed Pac-Man game today and I need to work.

“Doctors in Montreal can now prescribe their patients tickets to the orchestra” www.cbc.ca/radio/asitha...

Avg. of SARS-CoV-2 N1, N2 or other viral genetic markers normalized to Pepper Mild Mottle Virus as a fecal strength indicator; Samples collected by @ottawacity.bsky.social, tested & analyzed by Delatolla lab (uOttawa); Data i/o by @dougmanuel.bsky.social; Plots and tweet bot by @rnaguru.bsky.social

Ottawa WAVE (Wastewater Analytics for Viral Epidemiology) as of Oct 26. LEFT IMAGE: 7d trendline of SARS-CoV-2 signal since spring 2020 (A); Past year (B), and 2 months (C), inferred case incidence (D), and Reff (E) determined with ern (PMID: 38905266). RIGHT IMAGE: other viral traces