When it's so hot that your car catches fire on the road and you almost explode a gas station🔥🔥🔥

This paper combines two sort-of-crazy (in the best sense) approaches in cryoEM sample preparation: (1) Native, soft-landing electrospray ion beam deposition, followed by (2) deposition of amorphous ice, and reheating and freezing by laser flash melting. Has potential for time-resolved studies.

Two weeks left to apply!!!

NEW: Judge Burroughs GRANTS Harvard's requested TRO against the Trump administration, blocking it from revoking Harvard's ability to enroll international students during early court proceedings.

This is just an excellent preprint by Rasmus Jensen and colleagues from Julia Mahamid's lab @embl.org - a tour de force of Cryogenic electron tomography to do in cell structural biology in which they discover a new complex and solve its structure and function! www.biorxiv.org/content/10.1...

Someone posted this and I have been back to the original on repeat since 😄.. Captures perfectly the current mood, Imma losin' m'self in the sciencccccce 🤓🤓🤓🤓 youtu.be/xYtaBfwpg_k?...

Fellow ladies in science, as part of the disappearing species of international female scientists in France and perhaps every/elsewhere, I want to bring your special attention to our institute's open call for group leaders 👩‍🔬🦠🧪🔬🤓!!! Come join me in doing some coolicious science here 🙏!

🦠 Most #bacteria form #biofilms, protective communities that resist #antibiotics. Petya Krasteva's research explores the structure & behaviour of these biofilms, hoping to develop new #antimicrobial strategies. Read more 👉 buff.ly/YajqauG @krastevalab.bsky.social @iecb.bsky.social @CNRS.fr

Check our study in press in JACS on the use of solid-state #NMR to investigate polysaccharides in encapsulated pathogens, demonstrated on #Cryptococcus neoformans. Congrats to Alons Lends and all coworkers @iecb.bsky.social @cnrs.fr @univbordeaux.bsky.social pubs.acs.org/doi/10.1021/...

I am not a chemist and must be brain-dead after prolonged ogling of electron density clouds but how TF is this cellopentaose 🤔?

And another one bites the flux 🤘🤘🤘! Molecular replacement was tricky mostly because of Alphafold lacking imagination 😅! Beautiful home source data at 1.9 Å courtesy again of Xtal master @bkauffmann.bsky.social at our @iecb.bsky.social

Big News (a bit late 🙃): my group is moving to Düsseldorf @hhu.bsky.social Looking for next adventure w awesome colleagues @frunzkelab.bsky.social @kedrov-lab.bsky.social @apmweber.bsky.social Plz RT, positions available for #PhD & #postdoc w focus #CryoEM & #cdiAMP www.hhu.de/news-einzela...

Has been a while since we bothered to set up crystal trays, cryo-EM got us so spoiled 😄.. Not bad data either, home source dataset at 1.7 Å courtesy of our Xtal guru @bkauffmann.bsky.social at our @iecb.bsky.social 🤓🙏💠..

Welcome to Bluesky and thank you for this post 🙏. Indeed it was a real rollercoaster and this grant came as a lifeboat in the cold and lifeless ocean of public research in France, where institutional support is a net negative 😄. So we'll rock on for another 5 years while ignoring admin emails 🤘🤘🤘

OK, this is just a damn cool article 🤯 🦠 🔬 Infecting the cell nucleus, gaining nutrients, but preventing cell death! www.nature.com/articles/s41...

Happy to share our 📖 chapter on bacterial cellulose secretion! It took too long in press so it doesn't include our latest work on pEtN-cellulose secretion (doi.org/10.1038/s414...), which settled many open questions but nevertheless, hope you enjoy the read 🤓! link.springer.com/chapter/10.1...

That's the spirit, fuck bubbles as enjoyable as they might be 😄! Disclaimer: no personal energy to engage on that network meself 🙈🙈🙈

WowWowWow!!! Mechanism of histone H1 killing MRSA 🤓! Congratulations to all the authors 👌

Is this now happening for real? Are we acknowledging that SciTwitter transitions to BlueSci?!? 🤩 #bluesci #migration

@djuranoviclab.bsky.social will be Djuranovic lab at Brown University and newly formed RNA Center from July 1st 2025. Postdoc as well as staff scientist positions will be available. www.brown.edu/news/2024-02...

Now out in NSMB for those who missed our biorxiv back in 2023.. bacterial ESCRT-III-like #Vipp1 forms dynamic spiral filaments on membrane! Spirals are springs that drive 3D ring formation in the spiral centre! 😵‍💫 wonderful collaboration with Adai Colom’s lab and @rouxlab.bsky.social rdcu.be/dZSPQ

A CORDIS_EU feature of our @krastevalab.bsky.social 's ERC StG project BioMatrix on bacterial biofilm formation, which highlights the pivotal role of our institute's infrastructure! cordis.europa.eu/article/id/4...

Join us in December for a week of daily matinées in Molecular Biophysics 🤩!!! Speaker line-up below, the venue is our IECB amphitheater, doors open at 10:30 for a snack and chat before exciting science 🤓!

I put this here to acquaint our newly growing bsky network with our recent work on how the disordered translation factor eIF4B navigates accross its self-association landscape encoded by its peculiar sequence and plasticity. rdcu.be/dZCaJ

Seeing so many new members and groups here 🥰 I am resuming activity on my account to stay in touch with disillusioned colleagues and friends leaving X and to clean my feed from BS. Staying on X however, as I believe we need more outreach than ever and less, not more, bubbles in science and society!

Happy to share this 👶 peer-reviewed here: we crack how E. coli and proteobacteria assemble cellulose secretion systems to recruit proxies for polymer decoration and nanomolar c-di-GMP sensing and diversify an otherwise boring synthase with low c-di-GMP affinity and 1 sugar-1 linkage mechanism!

The latest completed work from our ERC StG BioMatrix is now online 🤩🦠🧫🔬🧪🤓: mechanisms for c-di-GMP sensing and synthase activation by proxy, pEtN-transferase recruitment and polymer modification: www.biorxiv.org/content/10.1...

Nice highlight of our recent research on intracellular scaffolds for bacterial cellulose secretion 😍😍😍🙏🙏🙏 Original works from my lab: Abidi et al. in Science Advances (2022, left) and Sana et al. in Current Biology (2023, right). www.cell.com/current-biol...

Still not used to this platform.. are there ways to bookmark posts? Are there ways to send DMs so that not everyone is spammed with private ruminations?

Kicking off the winter with our latest paper on bacterial cellulose (BC) secretion 🤩🤩🤩!! Roles for BcsD/CesD beyond BC crystallinity and more dedicated intracellular BcsD-Proline rich partner scaffolds for cellulose secretion across Proteobacteria: www.cell.com/current-biol...

Umbrella toxin particles produced by Streptomyces block mycelial growth of competing species www.biorxiv.org/content/10.1...

PvdL Orchestrates the Assembly of the Nonribosomal Peptide Synthetases Involved in Pyoverdine Biosynthesis in Pseudomonas aeruginosa. https://www.biorxiv.org/content/10.1101/2023.12.12.571276v1

Ever wondered how Pseudomonas aeruginosa "choose" between planktonic and biofilm lifestyles, at the molecular level? 🦠🔬🧐 #CryoEM Fantastic team work from the @krastevalab.bsky.social (and yes, I'm biased 😊) See the paper here: www.pnas.org/doi/10.1073/...

Our latest paper just came out at PNAS 🤩! Structures of the P. aeruginosa FleQ-FleN master regulators reveal large-scale conformational switching in motility and biofilm control. ERC-funded, as usual. www.pnas.org/doi/abs/10.1...

Naturally occurring CRISPRi proteins. In one case, phage use them to repress a host flagellin gene, which is then replaced with a version of the gene the phage carries. What a discovery!

Starting off here with a throwback to our last years paper on finally cracking crystalline cellulose secretion by the Gluconactobacter lineage: the longest studied c-di-GMP-responsive and EPS secretion system: www.science.org/doi/10.1126/...