It is a great privilege to be named Mencoff Family RNA Research Professor of Molecular Biology, Cell Biology and Biochemistry at Brown University. I am grateful to Mencoff Family, Brown University, Department of Molecular Biology, Cell Biology, and Biochemistry and Guiliani RNA Center.

Our RAPPL international patent application has just been published.

Visit from a friend and great colleague from Newcastle University. Sergey Melnikov will after snowy February in Providence talk tomorrow about hibernating ribosomes. Another talk at Brown RNA Center. #ribosomes #RNA

Great collaboration with Dougherty and Pavlovic Djuranovic labs on using MPRAs to look into non-coding mutations. Approaches for identification of 5′ UTR mutations impacting translation and protein production from neurodevelopmental disorder genes www.sciencedirect.com/science/arti...

On the way back from one week of great science, technology and therapeutic potentials of RNA in organization of Immagina Biotechnology S.r.l. and Massimiliano Clamer. Thanks for the great meeting to Max and the Immagina team. Looking forward to future collaborations. #RNA #tRNA #ribosome

A short synopsis of a recent Symposium at Brown University and inaugural Giuliani RNA Center meeting. It was an honor to be part of it. www.brown.edu/news/2025-09...

We are looking for a new postdoctoral fellow interested in the further development of our RAPPL technology and studies of translational complexes. Preference will be given to candidates with either structural biology or ribosome profiling backgrounds. www.nature.com/articles/s41...

Please join us for the Inaugural Brown RNA Center Symposium with a Keynote Speakers Melissa Moore and Craig Mello, guests Victor Dzau, Jack Reed and Subra Suresh, as well as more talks and panels including both scientific and industrial speakers and panelists. sites.brown.edu/rna-symposium/

Four days of science at @events.embl.org in Heidelberg for "Protein Synthesis and Translational Control" meeting.

Our RAPPL purification of ribosomes and associated material is out. I am really proud of this study in collaboration primary with Pavlovic-Djuranovic lab, as well as Jovanovic and Hashem Labs. This method aims to replace 60 years old purification of ribosomes by sucrose cushions rdcu.be/ezlhg

Our collaboration with Tony Mustoe's lab us out today. MPRA libraries and possible cryptic splicing in MPRA reporters, or reporters in general. The additional novelty here is that such events are controlled or influenced by AU-rich sequences. www.nature.com/articles/s41...

First RAPPL ribosome isolation at the new lab space at Brown University. Purification from a small culture of parasitic Eukaryote. Soon out... www.biorxiv.org/content/10.1...

@djuranoviclab.bsky.social is from today officially part of Brown University and Brown RNA center. We will have our new website soon. rna.brown.edu

Last month @uspto.bsky.social issued yet another patent to Slavica Pavlovic Djuranovic, Jessey Erath and @djuranoviclab.bsky.social for use of polybasic peptide or non-peptide moieties against malaria parasites. More soon...

Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (6/6). Archaea #1 www.biorxiv.org/content/10.1...

Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (5/6). Eukaryotic #3 www.biorxiv.org/content/10.1...

Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (4/6). Eukaryotic #2 www.biorxiv.org/content/10.1...

Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (3/6). Eukaryotic #1 www.biorxiv.org/content/10.1...

Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (1/6). Bacterial #2 www.biorxiv.org/content/10.1...

Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (1/6). Bacterial #1 www.biorxiv.org/content/10.1...

This was a fun collaboration with Maxim Arttyomov lab on how itaconate inhibits the active site of a peroxiredoxin enzyme. Thanks Maxim for including us in this. www.nature.com/articles/s42...

Ready for the next RNA session in @asbmb.bsky.social meeting. We start soon...

Ribosome data collected from a new and precious sample of 10,000,000 cells from a strange organism. All done using RAPPL, more soon... www.biorxiv.org/content/10.1...

3 years after terrible loss (two -80C freezers and all back-ups) of almost all of our collection of regular and mutant human, Drosophila, E. Coli and Plasmodium cells, we are still not finished with insurance.

One of the best single step purification preparations of protozoan ribosomes I've ever seen. Combination of RAPPL (biorxiv.org/content/10.1...) and a new technique. More at rna.ucsc.edu/rnacenter/ri...

🚨The triennial Ribosome Conference will be held June 22-June 27, 2025 in Pacific Grove, California. Registration is open - hope to see many #Ribosome #RNA aficionados there. #RNASky #AcademicSky 🧪 rna.ucsc.edu/rnacenter/ri...

Ok after @cryoclem.bsky.social we also tried Archaeal ribosomes (and some proteasomes) with RAPPL (www.biorxiv.org/content/10.1...) as well as some specific translation cycle complexes with new method.

After RAPPL (www.biorxiv.org/content/10.1...) we found another way to get ribosomes and other macromolecular complexes easier to the cryoEM grids. This time besides ribosomes p97 complexes were purified (back to the PhD times). Stayed tuned...

As I noted more to come from RAPPL (www.biorxiv.org/content/10.1...)

In addition to today's posted work by @cryoclem.bsky.social on Archaeal ribosomes we further expanded RAPPL (www.biorxiv.org/content/10.1...) on multiple other complexes that work with ribosomes, subunits or even off the ribosome. Soon more...

Thanks for sharing!

Some translating but stalled bacterial ribosomes. Tandem purification using RAPPL. Ready for cryoEM...

We are down to 2.32A and 2.61A for these specific ribosome complexes using tandem purification using RAPPL and tagged protein enrichment. In this case it was and hour and 45 minutes prep and 20-50 ml bacterial culture. www.biorxiv.org/content/10.1...

When your cryoEM run and data collection is not finished but still shows what you were looking for. Another one from 2ml culture.

More ribosome structures solved by RAPPL (collaboration with Pavlovic Djuranovic and Hashem Labs). This one is bacterial from 2ml culture and using method described in our preprint. www.biorxiv.org/content/10.1...

As I mentioned before, from July 2025 our lab and lab of Slavica Pavlovic Djuranovic will be joining newly formed RNA Center at Brown University. Our labs will be in the Department of Biochemistry and Department of Pathology and Laboratory Medicine. Here is the link for RNA center. rna.brown.edu

Congratulations to Dr.Courtney Jungers for successful PhD defense. Another PhD from @djuranoviclab.bsky.social

Bye Tokyo and Japan till next meeting. This was such a great experience. Greetings to all great scientists that we met over the last three weeks in Japan.

Second day of Ribosome meeting at the University of Tokyo. With Slavica Pavlovic Djuranovic.

With 2/3 RNA conferences done (tRNA 2024 and MBSJ 2024) we are leaving beautiful Kanazawa, Shirakawa-go and Fukuoka and we are heading to Tokyo and meeting on ribosomes at University of Tokyo. Again thanks to all hosts.

More RAPPL isolated ribosome complexes. Some are less complexed (first two images) same are more (last two images) complexed samples. Soon more input on these ones. Collaboration with Slavica Pavlovic Djuranovic and Yaser Hashem labs. #RNA #RNABiology #Ribosome

More from early morning walks in Kanazawa, and just before scientific sessions in tRNA meeting. #tRNA #RNA

Morning walk through Kanazawa.

Kanazawa and tRNA meeting, here I come. Hope to see a lot of new and also usual people there.

This one is a great collaboration with @astratman.bsky.social on characterization of U12-spliceosome gene ZCRB1, U12-intron splicing and its role in ciliogenesis and WNT-signaling. Halloween passed but we can still get headless zebrafish. #RNA #splicing #RNAbiology www.biorxiv.org/content/10.1...

One of the most ancient sequences in biology, the peptidyl transferase center of the ribosome, is not as conserved as we might have thought. #RNA #Ribosomes www.biorxiv.org/content/10.1...

@djuranoviclab.bsky.social will be Djuranovic lab at Brown University and newly formed RNA Center from July 1st 2025. Postdoc as well as staff scientist positions will be available. www.brown.edu/news/2024-02...

This preprint is a collaboration with Tony Mustoe's lab at Baylor Medical School and a little cautionary tale for everyone using MPRAs and possible cryptic splicing in such reporters, or reporters in general. This one is influenced by AU-rich sequences. www.biorxiv.org/content/10.1...

Ready for three scientific weeks and lot of RNA in Japan. Going from tRNA meeting www.trna2024kanazawa.jp in Kanazawa to Mol Biology Society of Japan meeting www.aeplan.jp/mbsj2024/en-... in Fukuoka to Tokyo Ribosome Meeting www.inada-lab.ims.u-tokyo.ac.jp/%E3%82%BB%E3... Thanks to all organizers!

Let's start this Blue sky account with this recent post from X. Collaborative work with Hashem, Jovanovic and Pavlovic Djuranovic Labs. A rapid, facile, and economical method for the isolation of ribosomes and translational machinery. Example from E.coli in figures www.biorxiv.org/content/10.1...