Using ampicillin as a selectable marker? You can skip the post-transformation outgrowth! Why? Because Amp targets cell wall synthesis, not protein synthesis, so the bacteria isn’t impeded from making the protein it needs to combat it! youtu.be/XZn8QG6dzgc

🗣️ 𝐼𝑛 𝑐𝑜𝑛𝑣𝑒𝑟𝑠𝑎𝑡𝑖𝑜𝑛 𝑤𝑖𝑡ℎ 𝐃𝐫. 𝐂𝐲𝐫𝐢𝐥 𝐙𝐢𝐩𝐟𝐞𝐥 Cyril Zipfel, a leading voice in plant immunity, reflects on plant immune recognition, his scientific journey, and what’s next for the field 🌱🧬 interviewed by @luisdeluna.bsky.social 👉 doi.org/10.1111/tpj.70688

At @elife.bsky.social you can now include explainer videos with every figure. Like going to a seminar while you engage with the paper. First example here elifesciences.org/articles/106... Click the arrows next to each figure to get a video of @mathiassablemeyer.bsky.social explaining it for you!

Thrilled to congratulate the newly minted Dr. Allahham on an outstanding viva! Many thanks to collaborators and the examiners for their invaluable support throughout the process. @ottlab.bsky.social @kleinevehnlab.bsky.social @hartman-plantlab.com

Proteoforms versus isoforms and the challenges of measuring them youtu.be/nRi4-3zl6IE

Happy New Year! P.S. May this motivate you to perhaps make learning your amino acids one of your resolutions?...

#OpenCloning is a an Open Source alternative to SnapGene/Benchling that supports automation and integration with other software ✅ Free 🔓 Open Source 🧬 More cloning methods than SnapGene 🤖 Can be automated with python 👨‍🔬 Built by a researcher — for researchers! 👉 Check it out at opencloning.org

Just wrapped up my last lecture of 2025. Interested in plant biochemistry and molecular biology? Slides are on GitHub: github.com/bpucker/teac... #OpenEducation #PlantSci @puckerlab.bsky.social

"Pseudomonas can make or break a happy phyllosphere microbiota" Daniela Ramírez-Sánchez & I discuss a neat study from the Gu lab on how different Pseudomonas strains influence the success of Fusarium pathogens in wheat by altering host pH kwnsfk27.r.eu-west-1.awstrack.me/L0/https:%2F...

Tony Burnetti and I recorded this super fun podcast in which we rank evolution's craziest adaptations. I think it turned out great! I might make this an exercise for one of my final evolution lectures next time I teach (dynamically rank / debate in class!). www.youtube.com/watch?v=JSum...

Are peer reviewers influenced by their work being cited? Version of record at @elife.bsky.social. Thorough and useful peer review - who needs and impact factor?! Links to paper and code/data ⬇️ 📄https://elifesciences.org/reviewed-preprints/108748 💻https://github.com/agbarnett/cited_reviewers

Undergrads in the UK, Ireland, Canada, and the US! One week left to apply for a funded internship in my lab! 📌 Details below 🔁 Please repost to spread the word!

Undergrads from Canada, Ireland, UK, & US: Don't miss the DAAD-RISE #plantscience funded internship at @ipbhalle.bsky.social with @snp2prot.bsky.social. Apply by Nov 30th: daad.de/rise/en/rise-germany/find-an-internship 🌱

Thrilled to share our latest review on receptor kinase processing in plants! 🌱 Huge congratulations to @adithya1972.bsky.social and Anna for leading this work, and a big thank you to our fantastic collaborator Martina-Ried Lasi for making this a truly rewarding team effort!

Periodic PSA: "western" should be lowercase in western blot – unlike the "Southern" blot, which was named for a person, the western blot was named for a laugh

A few years ago I made a slide showing the top 10 results if you search Google Image for "cartoon scientist". In my slide, 10/10 are white men. Someone asked if this is still true, so I tried again. Here below you have 24 images. 20 are men, 4 are women (progress!). 23 are white, 1 is blue 🤔

Rewriting the code of plant immunity go.nature.com/475WgV7

1/13 Thankfully, both you and your plants have a lot of sophisticated ways to fight off invading pathogens. In our new preprint, we describe a new way in which animals and plants share a common strategy to ward off harmful bacteria. www.biorxiv.org/content/10.1...

Have you ever wondered why pumpkins are never purple or blue like other plants? 🎃🌈 While most plants can produce pink, blue, and purple pigments (thanks to anthocyanins), the Cucurbitaceae family 🍈 🍉 🥒have lost all the pathway genes to produce these pigments. 🔗 doi.org/10.1101/2025...

💡 SPECIAL ISSUE VIEWPOINT 💡 Immune proteases are promising targets for protein engineering 🛠️ to boost disease resistance in plants 🌾 - Schuster et al. 🔗 doi.org/10.1093/jxb/... #PlantScience 🧪 @marischuster.bsky.social @aciattoni.bsky.social

My flatmate asked if I’ve ever made a GMO. This sparked a discussion making me realize how challenging science communication is and how scientists often lack training in it. I’m catching up with a podcast by @orlandodl.bsky.social that explains GMOs to a broader audience... while working with GMOs😅

I’m so proud to be a part of this OER that aims to lower the barrier for professors to incorporate molecular modeling & visualization into their courses (i.e. teaching students to interact w/3D models of proteins & other macromolecular structures &/or making custom figures)! doi.org/10.15781/m4z...

Stop citing journal names in your presentations - but PMIDs are too long (I will never get all of those numbers in the correct order!) Check out this awesome tool that takes your DOI from 10.1371/journal.pbio.3002882 to pw75. Much more accessible and easy for the audience!

Students at @leibnizipk.bsky.social have organised a well-crafted conference #PSSC-2025 in the previous few days. SNP2Prot members were able to join in this event and meet the Leibniz plant community🍀 @leibniz-gemeinschaft.de @unihalle.bsky.social @ipbhalle.bsky.social @unileipzig.bsky.social

@aciattoni.bsky.social representing the #ReceptorBiochemistry group @ipbhalle.bsky.social at #PSSC2025. Enjoy!

Really happy to be at the 20th Plant Science Student Conference #PSSC2025 @leibnizipk.bsky.social I am looking forward to exciting talks and discussions in the next few days.

I am happy to share our viewpoint on immune protease engineering! academic.oup.com/jxb/advance-...

Our authors can freely speculate the meaning and implications of their data in our new section: 💡 Ideas and Speculation 💡 buff.ly/0I8lpwz