Isomer-specific distribution of perfluorooctane sulfonate (PFOS) in hepatic zonation in mouse https://www.biorxiv.org/content/10.1101/2025.10.13.682167v1

WORM EMERGENCY!!! Our -80 compressor is failing and we’re not sure what will happen to our worm stocks at the current temp (-45C). They’ve been at this temp for 16 hours and we’re contemplating reviving all strains not currently in use to save them, or move them to a new -80. Anyone have advice??

That’s me! I’ll be highlighting some cool work with @brukercorporation.bsky.social’s Trapped Ion Mobility Spectrometry (TIMS) showing some neat isomer work during the workshop, and iprm-PASEF data at a poster (WP 266) so come see what myself and my lab are up to!

Finally getting good fluorosmurf assay results since I cut my fluorescein percentage from 2.5% -> 1%. 🪱

Weird question and I fully expect to not get a good answer, but when does the conference date for MAPSS usually get announced? My family wants to visit in late June/July and they go to campgrounds during their stays and they want to book early. I don’t want to tell them a date MAPSS might be on. 😅

Any C. elegans fan?

Super excited to see someone else try my method for analyzing PFAS using MALDI-TOF with TIMS! I’ve been having great results behind the scenes imaging sulfonates in mouse tissues and it’s awesome to see plant imaging done as well. www.sciencedirect.com/science/arti...

Nothing could have prepared me for how tiny microchannel plates are! Got to replace them in an icpTOF S2 and it was awesome. #teammassspec

#Celegans #Worms - I'm guessing sending whole labs to big conferences might be too expensive for many labs this year. I want to highlight this meeting will be FREE. VAI will cover all costs and lunch and if people still want night networking I will set something up if enough are interested.

Collaborators didn’t like the manuscript. Redoing a million experiments now. Delayed until March. Feels like the last few months of rushing this paper for grants and fellowship deadlines out were for nothing.

This is from my lab! I’m hoping my preprint will be out later this week or Monday! Seems like with grant submissions out of the way we’re finally submitting these papers. 🤣

Taking a break from designing lego mass spectrometers and made my stepdad (an electrical engineer who worked in renewable energy) a solar farm. Needed a mental break from submitting a P41 and editing this manuscript. Thank god both of those are done next week.

Unfortunately I can’t go to #Worm2025. I’ll instead be sticking to my midwest roots and going to MAPSS and a midwest C. elegans conference at Van Andel institutute in Grand Rapids, MI.

Updating 2 more supplemental figures tomorrow morning and finishing the abstract and conclusion before revising for the next week… so close to being done with this paper 🙏

Pairing microscopy images with my mass spec images… whoof this is tedious when I have 80 images of worms. Going to be a long Sunday for me 🤣

I need some #Celegans help part people. I have synchronized populations reaching day 1 gravid adulthood and I see differences in length by up to ~100um. Does C. elegans grow a little more once becoming gravid to reach its ~1mm length or… 1/2

Folks I may have just found the Lego parts to make a moveable C. elegans. Stay tuned for a potential build in the coming few days.

Ordering a new MCP for the Tofwerk S2 and I’m super excited to get the chance to crack it open and install it with a colleague in the coming weeks. Been awhile since and instrument vendor was chill enough to just send us the SOP and install it ourselves instead of bringing someone out to do it.

How hard do bacteria-eating nematodes bite in order to break the bacterial cell wall? Check out our new paper, led by Jason Casar & collab with Jen Dionne, using pressure sensing beads that change color upon compression (bluetorial coming soon) rdcu.be/d5n7v

2/5 main text figures done (currently doing fig 3), 4/6 supplemental figures done… we’re getting close people!

I really want to explore career options beyond the USA after I finish my PhD… Canada, Sweden or Australia/New Zealand sound cool. I’m just sick of life here and want to have a comfortable life with my wife doing research.

I really need to stop publishing around the holidays 🤣

yep. has happened to me.

"You will be visited by three spirits". The three spirits:

Enterobactin carries iron into C. elegans and mammalian intestinal cells by a mechanism independent of divalent metal transporter DMT1 https://www.biorxiv.org/content/10.1101/2024.12.20.629725v1

Taking a week off for the holidays is going to be nice. I needed a break from pushing through data collection and I need time to make figures. Should have all data collected Jan. 1st which will be a neat way to start the new year. 😃

Interviewer: Can you explain this gap in your resume? Me: I was waiting for the NMR

Parts came in finally! Very happy with the blue pieces I chose. #teammasspec #chemsky

Super secret C. elegans mass spec imaging manuscript submission timeline has shifted slightly. Some authors will be unavailable to review the manuscript until the week or Jan. 6th-10th so we hope to have it submitted/accepted the following week. Once that’s done we’ll upload a preprint!

C. elegans and Mtb! www.etsy.com/shop/Microbe...

tis the season

Failed to make a good looking NGM plate out of legos so it’s back to Zeiss and Leica microscopes for the nematode folks.

Amazing C. elegans cake made by my talented PhD student! #worms

Next model: Bruker D8 Venture Single Crystal XRD (request from friend). Contains simplified Photon CPAD detector and X-ray source with space for a small colored stud of whatever color you want your crystals to be. A reference photo from internet is attached. #Chemsky

The Mendoza lab is hiring a Research Assistant at the University of Colorado Boulder!! Come join our brand new lab as we launch our group! We study zinc homeostasis using a variety of techniques in C. elegans and human models. The view in Boulder ain't bad either! Reach out with questions!

I think I’m going to make some non-mass spec lego stuff for a bit. Maybe a little vivarium and an NGM plate… maybe my Zeiss I use, too? I’ll be back to make other mass spec eventually. Will probably do Something Sciex or Shimadzu.

This has been made, and it came out perfectly. Still waiting on the ICP-TOF parts to come in (you can see the current build behind my hand LOL) #teammassspec #Chemsky

Final product has been made! It’s a lot smaller than I thought it would be but it’s a perfect paper weight for a desk! #teammassspec #Chemsky

Two stages of writing: 1) This shouldn't take too long 2) Oh no

On the importance of detailed protocols.

Adding to the #Celegans community with a sneak peak of my next paper coming out in December. I develop mass spectrometry imaging methods for C. elegans imaging as part of my thesis and this paper should be a big deal when it drops. Not spoiling what I’m imaging yet but the community will love it!

I got my first citation, and they didn’t say my paper was shit!! 😊😊

Doubt I’ll make my personal deadline for this paper (before Christmas) but January seems doable. Really need this to be published with minimal revisions for upcoming fellowships/grants 🤣 #chemsky #teammassspec

I’m never attending a conference in a red state LMAO I will 100% be skipping ASMS 2026 in Texas

Master thread for Lego analytical instrument designs. First up: Updated Bruker MALDI-timsTOF fleX with reference image attached. 76 parts total.

I’m making lego instrument sets like Thermo, but for equipment my lab uses. First up: Bruker MALDI-timsTOF fleX. 60 pieces total, but I need to double check the color blue I want. If people are interested in sets I make I’ll share the part lists and instructions to build. #teammassspec

Cryptically leaving this post for #Chemsky and #teammassspec to decipher. It’ll all make sense in the next 6 months 👀

Me in lab after 10 hours: