These are also class 3 PDZ binding motifs.

"Look, we know we told you all loudly and repeatedly to fuck off for two years, and talked down Canadian institutions as being low-quality for the same space of time, but that's all behind us, please won't you come?" - A government that can't think more than one news cycle ahead

#proteomics_101 The C-terminal sequence tag KDEL has gotten a lot of ink for its active retention in the endoplasmic reticulum. But it is part of a larger pattern: the table below shows some other selected C-terminal tags & the # of soluble ER proteins/total # of proteins with those tags.

I

Today's slime mould,Trichia crateriformis, which has opened up its top to release its spores (by a process known as circumsessile dehiscence!)

For some reason, this sort of political flexibility always reminds me of a line from Deadwood decrying a similar lack of ethical constancy by a local official: "the camp sheriff can be bought off for half a can of bacon grease" www.cbc.ca/news/politic...

All of the 'omics (proteomics included) are convinced that if you overlay enough sh*t onto your data, surely a beautiful pony must emerge. www.sciencedirect.com/science/arti...

#proteomics_101 The protein subunits retained in the ER via the KDEL tagging mechanism are all involved in the folding & maturation of the many proteins that are secreted via ER-Golgi pathways. They tend to be very abundant in proteomics data, especially in secretory cells & cancer tissue.

Hotel magnate Thomas Pritzker will step down as the executive chairman of Hyatt Hotels after details of his affiliation with Jeffrey Epstein were revealed in documents related to the burgeoning investigation of ties between the notorious sex trafficker and the elite and powerful.

#proteomics_101 In order for the KDEL-tagged protein retention mechanism to work, the protein must be in solution, i.e., not bound to a membrane by a transmembrane domain or a PTM membrane anchor. All of the 11 proteins with KDEL tags fit the bill.

Cladonia sp #lichen. #newfoundland, Canada. #fungi #fungifriends The lichen is about 3cm top to bottom.

NBC News: 'National Institutes of Health faces leadership vacuum as director positions sit open' 'More than half of the NIH's institutes and centers don’t have permanent directors, giving the Trump administration an unusual opportunity to reshape the agency...' www.nbcnews.com/health/healt...

#proteomics_101 The C-terminal sequence "…KDEL" marks a protein for active retention in the endoplasmic reticulum. But for that to work, these proteins must be in the ER to begin with. Therefore, they need an N-terminal signal sequence that allows them to be translated through the ER membrane.

Dung moss, Tetraplodon mnoides. As the common name suggests, it often grows on animal dung, but can also grow on bones, antlers and other substrates. Always an eye catcher for its striking colours. #Newfoundland, Canada. #moss #bryophyte

Cladonia lichens. NWT, Canada. #lichen #fungi #fungifriends

#proteomics_101 The C-terminal sequence "…KDEL" indicates that the resulting protein will be actively retained in the endoplasmic reticulum. List 3 other characteristics of proteins with KDEL retention tags.

Protein event of the year - sign up today! See you at beautiful Palazzo dei Normanni in Palermo for the FEBS 2026 Protein Termini Workshop. #ProteinTermini #Proteostasis #ProteinModifications #StructuralBiology #PalazzoDeiNormanni #Palermo2026 proteintermini.org/meeting/

Just got my Public Lending Right money from Canada Council. And for the firs time ever, it's pretty good money ($1500). This is thanks to you taking my books out at the library. Keep doing it! And do it for your favourite authors! Even if you don't read the books!!

The polar vortex is still elongated, but it has spun eastward so that it no longer effects North America. It's your turn, Europe!

Bluesky is the new science Twitter, new study by @whysharksmatter.bsky.social and Julia Wester concludes! "Results show that for every reported professional benefit that scientists once gained from Twitter, scientists can now gain that benefit more effectively on Bluesky than on Twitter."

Between the feds cutting student grants in half and Ontario - which makes up something like 60% of the non-Quebec Canada Student Financial Assistance Plan "zone" cutting them by 60-70%, I am pretty sure we will be talking a lot more about student debt in 2030 than we have at any time since 1998.

Ontario needs some opposition @ontarioliberal.bsky.social Students!! 🇨🇦

#proteomics_101 Classification Determinant 44 is a type I membrane protein on the surface of many cells, particularly leukocytes & fibroblasts. Like many other plasma membrane proteins of this type, its activity can be abruptly halted by cutting off the nward region, which ends up in the urine.

Cladonia sp lichen, about 3cm high. #Newfoundland, Canada #fungi #fungifriends #lichen

That tracks 🤣 Just about smack in the middle of "proteomics mass spec"

#proteomics_101 NECTIN2 is a type I membrane protein: a signal peptide (1-31), an extracellular region with 3 tandem Ig domains, a TM (361-383) & an intracellular domain with pS/Y acceptors. Its sequence in urine is a proteolytic fragment of the extracellular region.

L’émissaire de Donald Trump à Minneapolis, Tom Homan, annonce la fin des opérations anti-immigration

#prteomics_101 NECTIN1 is a type I membrane protein: an nward extracellular region with 3 tandem Ig domains, a TM (356-378) & an intracellular domain slathered with pS/T/Y acceptors. Its abundance in urine is correlated with the rate of proteolytic removal of the extracellular region.

Slime mold Stemonitopsis typhina. NWT, Canada. #myxomycetes #fungifriends

A certain Austrian physicist would really be upset that almost nobody understood his (rather cruel) joke. www.popularmechanics.com/science/a702...

#proteomics_101 Clusters of Differentiation 27 is a type I membrane protein (TM: 189-211). Discuss how this single structural domain results in the changes in observed peptides shown below for T cells v urine. Which of these peptides should be included in DIA spectral libraries?

Someone should come up with a methodology of ranking US universities by their degree of appalling behaviour since January 2025. Figuring out a decent set of indicators and weights would be challenging but not impossible. Who's game?

The soft corruption of handing out private contracts without the modicum of management ability required to oversee said contracts.

And yesterday brought me a slime mould, the tiny pinheads of Comatricha nigra in vast profusion in our local Kent woodland. #slimemould #slimemold #fungifriends #naturephotography #myxomycetes #mycology

ByteMe seems like a better company name...

BytePlus has joined the list of so many other cloud services that I now block with extreme prejudice.

Look up.

Truly shameful to see how far the Liberals have fallen from a time when even Pierre Trudeau, for all his many faults, was willing to intentionally pursue diplomatic friendship with Cuba in direct defiance of US foreign policy as an assertion of Canadian sovereignty.

There are 3000 Canadians currently in Cuba and we cant be bothered to do something to help Cubans.

Told you.

#proteomics_101 Compare & contrast the signals from the InterCellular Adhesion Molecule 3 in T cells v urine. The protein (aka CD50) occurs on the plasma membrane of leukocytes with a cward TM domain (487-509). How would you interpret a differential increase in ICAM3 signal in the urine?

Stemonitis sp Slime Mold. NWT, Canada. #myxomycetes #fungifriends The larger slime mold is about 2cm high.

I am what I am what I am. bluesky-map.theo.io

#proteomics_101 Explain the biological processes resulting in the observed differences between observations of IntraCellular Adhesion Molecule 1 (ICAM1) in lung tissue v urine. How would you use this knowledge to understand observations in blood plasma?

Not really sure if I am an "independent researcher" or an "influencer": can you be both? I think I am too old to be an influencer, but independent researcher has a real Joe Rogan vibe to me.

A developing (not fully mature) Lamproderma sp slime mold NWT, Canada. #myxomycete #fungifriends

Russia is leaving Bologna