Steven Burgess
@sjb287.bsky.social
π€ 1488
π₯ 428
π 138
Assistant Professor UIUC, plant biology, evolution
#synbio
#photosynthesis
#plantscience
An interesting and provocative preprint by the Johnson group that challenges the decades old hypothesis that PGR5-cyclic electron flow is required to balance ATP/NADPH requirements for carbon fixation
@biorxiv-plants.bsky.social
worth a read
doi.org/10.1101/2025...
#photosynthesis
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Disequilibrium between chloroplast proton motive force and ATP levels in Arabidopsis
Current dogma holds that CO2 fixation by photosynthesis requires additional ATP production via PGR5-dependent cyclic electron transfer (PGR5-CET) to augment the NADPH and ATP produced by linear electr...
https://doi.org/10.1101/2025.11.12.687978
4 days ago
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Nice paper characterizing rubisco mutants
www.biorxiv.org/content/10.6...
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The fitness landscape of a Form II rubisco in a photosynthetic bacterium guides engineering of oxygen tolerance
Rubisco is an important but challenging protein engineering target. Fast and selective rubiscos could enhance photosynthesis in plants and accelerate biobased production processes. To facilitate engin...
https://www.biorxiv.org/content/10.64898/2025.12.07.690893v1
22 days ago
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#plantsynbio
peeps, has anyone played around with cell penetrating peptides? some of the papers look fairly convincing for delivery of DNA e.g. 10.1021/acs.biomac.8b00323. but am curious why not used more widely. Viruses and agro are just easier for most applications?
26 days ago
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Steven Burgess
noam
about 1 month ago
π±π¨ Funded PhD for UK students π¬π§πΏ Take a look at this Plant BioDesign Doctoral Training Program. Come work with me and
@michaelwebster.bsky.social
to study plant rubiscos. High throughput biochem at Cambridge and Cryo-EM techniques at the John Innes Centre.
www.findaphd.com/phds/project...
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Engineering plant rubisco enzymes through high-throughput enzyme assays and cryo-EM (Plant BioDesign Cambridge project) at University of York on FindAPhD.com
PhD Project - Engineering plant rubisco enzymes through high-throughput enzyme assays and cryo-EM (Plant BioDesign Cambridge project) at University of York, listed on FindAPhD.com
https://www.findaphd.com/phds/project/engineering-plant-rubisco-enzymes-through-high-throughput-enzyme-assays-and-cryo-em-plant-biodesign-cambridge-project/?p192163
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Steven Burgess
RenΓ© Inckemann
2 months ago
Very excited to finally share my PhD paper, about advancing
#chloroplast
#synbio
through high-throughput plastome engineering of
#Chlamydomonas
. Huge thanks to the whole team!
www.nature.com/articles/s41...
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Steven Burgess
Matthew Terry
2 months ago
I have a PhD studentship available to work on PIF proteins in wheat with Stephen Pearce
@rothamsted.bsky.social
and
@colleendrapek.bsky.social
at Phytoform labs. Any reposts greatly appreciated.
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Steven Burgess
ι΅ε¨ (Min Ya; Minya) PhD
about 1 month ago
Very happy to share that this paper is now online on
@currentbiology.bsky.social
!! π₯³π§ͺ Check out the final published verion here:
www.cell.com/current-biol...
#mimuluspropaganda
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New preprint - it has been my sad duty to help get this out after the passing of Steve Long and Amy Marshall-Colon. Testing combined effects of VPZ phenotype and eCO2 in soy (attempt to mimic improving both light use efficiency and CO2 assimilation). What exactly VPZ is doing remains unclear 1/n
about 1 month ago
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Work started by Don Ort's lab. How is rubisco activase regulated? There are suggestions that phosphorylation acts redundantly with redox to control activity. This paper shows if it does, this is likely limited to Arabidopsis. The role of PTMs (if any) in regulating Rca is still unclear
#plantsci
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about 1 month ago
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Pls help: I've read in several papers about use of kits from "Genmed Scientifics" which is quoted as a US based company, although the city quoted varies. No amount of Google searching can actually find this company, or the kits. Does anyone know what this is? I don't think it's fraud, but its weird
3 months ago
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Steven Burgess
Greg Copenhaver
3 months ago
The
@uncchapelhill.bsky.social
Department of Biology is searching for tenure-track Assistant Professor in Plant Molecular Biology. Chapel Hill is a wonderful place and Biology is an amazing group of people. To apply, see:
unc.peopleadmin.com/postings/307...
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Steven Burgess
Ben R Lee, PhD
3 months ago
π¨ Please repost widely!π¨ I am recruiting a PhD student to join my lab next fall to work on forest and climate change ecology projects in southern Appalachia! Come join me in one of the most beautiful, biodiverse, and understudied parts of North America. Details below and happy to answer questions
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Interesting read -10.1093/plphys/kiaf465 "Allelic variants of IRKI contribute to photosynthetic efficiency by regulating rubisco activase in Populus" there is a lot we don't know about regulation of photosynthesis here a receptor like kinase interacting protein has a role
@plantphys.bsky.social
3 months ago
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Steven Burgess
Lancaster Plant Biology
3 months ago
New
#PhD
opportunities! NorthWestBio projects now online for 2026! Including a
#photosynthesis
project w/
@marjorielundgren.bsky.social
looking at how photosynthetic diversity may affect future crop nutrient quality. Plant projects and the full set of projects available here:
tinyurl.com/529yz4jd
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FindAPhD : NorthWestBio Fully Funded Doctoral Training Programme Studentship 2026 at University of Glasgow
Apply for a PhD: NorthWestBio Fully Funded Doctoral Training Programme Studentship 2026 at University of Glasgow
https://tinyurl.com/529yz4jd
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Steven Burgess
Ben Long
3 months ago
We really enjoyed putting together this Darwin Review for JXB. Fun to think about how carboxysomes work and why plants don't have them.
@jxbotany.bsky.social
Function, Evolution, and Future of Carboxysomes url:
academic.oup.com/jxb/article-...
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The Function, Evolution, and Future of Carboxysomes
Abstract. Since their discovery as rubisco-containing compartments more than 50 years ago, significant breakthroughs have advanced our understanding of car
https://academic.oup.com/jxb/article-abstract/doi/10.1093/jxb/eraf425/8266333
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Steven Burgess
Xinyou Yin
4 months ago
Just came across this paper "Dual-cycle CO2 fixation enhances growth and lipid synthesis..."
www.science.org/doi/10.1126/...
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Dual-cycle CO2 fixation enhances growth and lipid synthesis in Arabidopsis thaliana
Carbon fixation through the Calvin-Benson-Bassham (CBB) cycle accounts for the majority of carbon dioxide (CO2) uptake from the atmosphere. The CBB cycle generates C3 carbohydrates but is inefficient ...
https://www.science.org/doi/10.1126/science.adp3528
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I'm deeply saddened. A tireless champion of plant
#synbio
and man of integrity. His insistence on focusing on real-world problems, calling out BS and bringing together lab and Ag, industry and academia will be sorely missed
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4 months ago
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We are hiring! The
#PlantBio
department at UIUC is looking to recruit an Assistant Professor in Plant-Fungal interactions. Application deadline is October 31st
@dallingjim.bsky.social
see the link below for more details:
illinois.csod.com/ux/ats/caree...
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Assistant Professor- Department of Plant Biology
Duties & Responsibilities
https://illinois.csod.com/ux/ats/careersite/1/home/requisition/14759?c=illinois&sq=1031975&referralToken=MX8lYTz4-EapwaM-jNL-bA
4 months ago
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Steven Burgess
Gozde Demirer
4 months ago
Big news from the Demirer lab: first 4 research papers are preprinted! Check them out, feedback welcomed: eCIS for plant protein delivery:
tinyurl.com/mrx4d878
R2 for targeted insertion:
tinyurl.com/5n73za6h
Transient expression:
tinyurl.com/38p2ud6w
Chemotaxis assay:
tinyurl.com/3nw9haes
More β¬οΈ
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Steven Burgess
Lars Γstergaard
4 months ago
π
my.corehr.com/pls/uoxrecru...
Join us and be part of shaping the future of
#plantscience
at Oxford as we move to the new Life and Mind Building
lifeandmind.web.ox.ac.uk/home
(recognised for its potential to change the World
edition.cnn.com/2025/01/01/s...
)
@biology.ox.ac.uk
www.biology.ox.ac.uk
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'
https://my.corehr.com/pls/uoxrecruit/erq_jobspec_version_4.jobspec?p_id=180329
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Steven Burgess
Sandy Hetherington
5 months ago
A great example of how comparative genomics across the full range of vascular plant lineages can be used as a catalyst for gene discovery! Exciting new single cell datasets for gymnosperms, ferns and lycophytes! The scale of this study is really amazing!
www.cell.com/cell/fulltex...
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A unified cell atlas of vascular plants reveals cell-type foundational genes and accelerates gene discovery
A cross-species single-cell atlas highlights a core subset of cell-type foundational genes associated with major vascular plant cell types, enabling the identification of hidden cell types and the dev...
https://www.cell.com/cell/fulltext/S0092-8674(25)00858-X?rss=yes
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www.nature.com/articles/s41...
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Assembly-dependent translational feedback regulation of photosynthetic proteins in land plants - Nature Plants
Algae fine-tune protein synthesis to assembly demands of photosynthetic complexes. In land plants, this feedback control is conserved in Rubisco and partly in photosystem II, but varies or is absent i...
https://www.nature.com/articles/s41477-025-02074-x
5 months ago
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Steven Burgess
Mary Williams @PlantTeaching
5 months ago
#PlantScience
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Steven Burgess
ι΅ε¨ (Min Ya; Minya) PhD
5 months ago
Iβm proud to present the main work from my postdoc, 3+ years in making from scratch, my ultimate
#MimulusPropaganda
up till now: The Moment That Symmetry Breaks.
#plantscience
#development
#imaging
#microscopy
π§ͺπΈπ¬ w. Captain Yaowu &
@biancatash.bsky.social
www.biorxiv.org/content/10.1...
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Steven Burgess
Liana Lareau
5 months ago
This preprint from Helen Sakharova is one of the coolest things to come out of my lab: βProtein language models reveal evolutionary constraints on synonymous codon choice.β Codon choice is a big puzzle in how information is encoded in genomes, and we have a new angle.
www.biorxiv.org/content/10.1...
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Protein language models reveal evolutionary constraints on synonymous codon choice
Evolution has shaped the genetic code, with subtle pressures leading to preferences for some synonymous codons over others. Codons are translated at different speeds by the ribosome, imposing constrai...
https://www.biorxiv.org/content/10.1101/2025.08.05.668603
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Steven Burgess
Conor McClune
5 months ago
A truly enabling new approach by
@taralowensohn.bsky.social
Will Cody and
@sattelylab.bsky.social
to probe plant genetics at scale. Single-gene-per-cell delivery coupled to an effective transcriptional selection system
www.biorxiv.org/content/10.1...
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Steven Burgess
Elisabeth Bik
5 months ago
Fantastic new paper by
@reeserichardson.bsky.social
et al. An enormous amount of work showing the extent of coordinated scientific fraud and involvement of some editors. The number of fraudulent publications grows at a rate far outpacing that of legitimate science.
www.pnas.org/doi/10.1073/...
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Steven Burgess
Johnathan Napier
5 months ago
Great to see our collaborative study with Ed Cahoon and colleagues on the front cover of PBJ, describing our successful efforts to engineer the accumulation of astaxanthin in GM camelina seeds. Astaxanthin is a ketocarotenoid that is a powerful antioxidant & used in aquaculture feeds as a pigment.
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Steven Burgess
Mary Williams @PlantTeaching
5 months ago
Lots of talk about teaching yesterday at
#PlantBio2025
. A few resources that might be useful. 1) From LUCA to Lily: 12 perspectives for teaching about plants
blog.aspb.org/from-luca-to...
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From LUCA to Lily: 12 perspectives for teaching about plants | Plant Science Today
The other day I was talking to a friend about the need to demystify plants, so that teachers feel as confident in their teaching of plant biology as they do about animal biology. I wonder if sometimes...
https://blog.aspb.org/from-luca-to-lily-12-perspectives-for-teaching-about-plants/
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Steven Burgess
Mary Williams @PlantTeaching
6 months ago
This is a great program. We started in in 2016 and it just gets better and better! Check out some past fellows who are on Bluesky:
@philcarella.bsky.social
@mattbnt.bsky.social
@psaima.bsky.social
@fromani.bsky.social
@sjb287.bsky.social
@aribidopsis.bsky.social
@cavycavs.bsky.social
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Steven Burgess
Maciek Wiatrak
6 months ago
π₯ Excited to introduce Bacformer π¦ - the first foundation model for bacterial genomics. Bacformer represents genomes as sequences of ordered proteins, learning the βgrammarβ of how genes are arranged, interact and evolve. Preprint π:
biorxiv.org/content/10.1...
π§΅ 1/n
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Steven Burgess
The Arabidopsis Information Resource (TAIR)
6 months ago
"Postdoctoral Researcher in Organelle Synthe4c Biology at UC Riverside, Riverside, CA, USA" Read more here:
https://arabidopsis.org/news/jobs/view?file=Yu_7-21-25.pdf
#PlantSciJobs
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Steven Burgess
Antony Dodd
6 months ago
Two amazing opportunities at the
@johninnescentre.bsky.social
add a skeleton here at some point
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Steven Burgess
Karas Lab
6 months ago
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www.nature.com/articles/s41...
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Guard cells on the adaxial and abaxial leaf surfaces use different compositions of potassium ion channels to drive light-induced stomatal opening - Nature Plants
This study combines single-cell RNA sequencing, in silico OnGuard simulation and genetic modification approaches to reveal distinct compositional differences in the potassium ion channel between adaxi...
https://www.nature.com/articles/s41477-025-02026-5
6 months ago
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Steven Burgess
Prof Ros Gleadow FAA π
6 months ago
My department has open positions at Level B (Lecturer) or Level C (Senior Lecturer), - one position in Genomics and two in Ecology.
#PlantSciJobs
Applications close on Thursday 7 August Aussie time
careers.pageuppeople.com/513/cw/en/jo...
careers.pageuppeople.com/513/cw/en/jo...
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Job Search
https://careers.pageuppeople.com/513/cw/en/job/679703/senior-lecturer-lecturer-in-genomics
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Steven Burgess
Ben Long
6 months ago
A perspective: some relationships between the biochemistry of photosynthesis and the gas exchange of leaves (Planta 153, 376β387)
link.springer.com/article/10.1...
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A perspective: some relationships between the biochemistry of photosynthesis and the gas exchange of leaves (Planta 153, 376β387) - Planta
The Planta paper βSome relationships between the biochemistry of photosynthesis and the gas exchange of leavesβ explored the relationship between gas exchange measurements of CO2 assimilation rate and...
https://link.springer.com/article/10.1007/s00425-025-04761-7
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Steven Burgess
Mark A. Hanson
6 months ago
The "reproducibility crisis" in science constantly makes headlines. Repro efforts are often limited. What if you could assess reproducibility of an entire field? That's what
@brunolemaitre.bsky.social
et al. have done. Fly immunity is highly replicable & offers lessons for
#metascience
A π§΅ 1/n
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Steven Burgess
Graham Coop
6 months ago
Looking for investors in my CRISPR company recreating the golden ram. Relive the days of Jason and the argonauts. Come get fleeced!
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Steven Burgess
Joel Sharbrough
6 months ago
Please RT! I'm hiring a postdoc to work in my new lab at UCSB (!) on an NSF-funded project investigating the phenotypic consequences of WGD for photosynthesis and respiration. Check out the full posting here:
recruit.ap.ucsb.edu/JPF02986
And more info about the lab here:
sharbroughlab.com
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Nice work showing it is possible to engineer increased oxygen tolerance of fast rubiscos using in vivo directed evolution via
@pnas.org
doi.org/10.1073/pnas...
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In vivo directed evolution of an ultrafast Rubisco from a semianaerobic environment imparts oxygen resistance | PNAS
Carbon dioxide (CO2) assimilation by the enzyme Ribulose-1,5-bisphosphate Carboxylase/Oxygenase (Rubisco) underpins biomass accumulation in photosy...
https://doi.org/10.1073/pnas.2505083122
6 months ago
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Steven Burgess
noam
6 months ago
Specificity is still really tricky, the underlying mechanism remains almost entirely unexplained and red algal assembly is not yet understood (as you point out). But the field is moving quickly!
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Steven Burgess
Laura Gunn
6 months ago
While we don't know how to assemble Griffithsia Rubisco yet, this expansion in SynBio capabilities (from Archer and friends) are very exciting - the high throughput engineering capabilities that they enable definitely bring us substantially closer to engineering higher specificity Rubisco.
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Further advanced in synbio systems for expression of plant rubiscos in E. coli, exploring importance of Raf1 variants:
academic.oup.com/jxb/article-...
and accompanying perspective via
@jxbotany.bsky.social
doi.org/10.1093/jxb/...
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Effects of chaperone selectivity on the assembly of plant Rubisco orthologs in E. coli
We used an E. coli system to examine recognition of plant Rubisco chaperones for different Rubiscos. Identified factors influencing chaperone selectivity e
https://academic.oup.com/jxb/article-abstract/76/10/2809/8101346?redirectedFrom=fulltext
6 months ago
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New from the Stern lab, KO of both rubisco activase isoforms in setaria. Beta necessary despite C4 pump, but role of heat inducible alpha remains enigmatic
@jxbotany.bsky.social
doi.org/10.1093/jxb/...
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The Ξ± form of Rubisco Activase supports photosynthesis during heat stress in the absence of the Ξ² form in Setaria viridis
Abstract. Rubisco activase (RCA) facilitates the removal of inhibitors from the Rubisco active site and is essential for plant growth at atmospheric CO2. H
https://doi.org/10.1093/jxb/eraf289
6 months ago
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Seen a number of groups working on this, but impressive work to produce transgene free heritable genome editing that avoids tissue culture in monocots. Importantly has potential for a range of species
www.nature.com/articles/s41...
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Transgene- and tissue culture-free heritable genome editing using RNA virus-based delivery in wheat - Nature Plants
Efficient delivery systems are urgently needed for genome editing in monocot plants. Here, this study develops a system of virus-induced genome editing in tillers (ViGET) to achieve heritable editing ...
https://www.nature.com/articles/s41477-025-02023-8
6 months ago
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Cool preprint - what is the boundary between virus and life? Following the recent discovery of the nitroplast there are lots of interesting things to be discovered in marine microbe communities
www.biorxiv.org/content/10.1...
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A cellular entity retaining only its replicative core: Hidden archaeal lineage with an ultra-reduced genome
Defining the minimal genetic requirements for cellular life remains a fundamental question in biology. Genomic exploration continually reveals novel microbial lineages, often exhibiting extreme genome...
https://www.biorxiv.org/content/10.1101/2025.05.02.651781v1
6 months ago
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reposted by
Steven Burgess
Max Planck Institute of Molecular Plant Physiology π±π§ͺ
8 months ago
New research by
@egonzalezduran.bsky.social
shows that DSB repair suppresses gene transfer from chloroplasts to nucleus. With DSB repair impaired, transfer rates jump 20Γ β revealing how plants protect genome stability, with lessons beyond plant biology. Read:
nature.com/articles/s41477-025-02005-w
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Suppression of plastid-to-nucleus gene transfer by DNA double-strand break repair - Nature Plants
Inactivation of double-strand break repair pathways greatly increases the integration of plastid DNA into the nuclear genome of tobacco plants, highlighting the mutagenic potential of organellar DNA a...
https://nature.com/articles/s41477-025-02005-w
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reposted by
Steven Burgess
Sternberg Lab
8 months ago
1/10 Today in
@science.org
in collaboration with the Liu group we report the development of a laboratory-evolved CRISPR-associated transposase (evoCAST) that supports therapeutically relevant levels of RNA-programmable gene insertion in human cells.
drive.google.com/file/d/1I-Ub...
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Steven Burgess
Sophien Kamoun
8 months ago
Tweeps, please spread the word. We're still looking at recruiting a structural biologist (any level) to study plant-pathogen protein complexes especially in the context of coevolution. Direct inquiries welcome. Please include a cover letter. Applicant from US welcome?
medium.com/@kamounlab/2...
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