My project at @i-med.ac.at focused on lysosomal proteins which show an uneven distribution between perinuclear and peripheral pools (www.biorxiv.org/content/10.6...) Here's one identified perinuclear lysosomal protein, EPDR1-mCherry (magenta), expressed in U2OS cells alongside LAMP1 (green).

My postdoc paper is now out as a preprint 📜 What actually happens inside cells when lysosomes are repositioned? We show that changing lysosome position has functional consequences — and we also uncover specific lysosomal proteins that become enriched or depleted depending on their localization.

BLOC-1 and BORC help move and position lysosomes inside cells. New work from the Haselbach and Clausen labs shows they are not uniform complexes, but families of related protein assemblies. Published in PNAS: https://www.pnas.org/doi/10.1073/pnas.2515691123

I tried an even harder example on Gemini Pro image generation and this is quite scary/amazing. I asked for a microscopy image of around 20 HeLa cells, GFP tagged 20% nuclear, 10% membrane, +1 nuclear staining, + overlap. Image below and prompt in the following post.

In our cover, Waich et al. record progress for early-onset O2HE Syndrome–their analysis of the UNC45A c.710T>C 37 missense variant uncovered its pathogenic mechanisms & recessive 46 characteristics and may aid in the development of targeted therapies: buff.ly/sU0qlNx

Excellent set of guidelines for anyone developing software for life scientists, from @jwpylvanainen.bsky.social, @lankylaste.bsky.social and @guijacquemet.bsky.social journals.biologists.com/jcs/article/...

An old timelapse I dug up... U937-derived macrophages bathed in green and red dextran... the green dextran is pH sensitive, leading to the appearance of mostly red lysosomes.

NEW: Russia Has Failed to Break Ukraine: isw.pub/RussiaRetros... Russia dedicated staggering amounts of manpower and equipment to several major offensive efforts in Ukraine in 2024, intending to degrade Ukrainian defenses and seize the remainder of Donetsk and Luhansk oblasts. (1/3)

I actually tested these two and also SamCell (recently on Biorxiv). Here's the quick and dirty results - might still be finetuned, and I might have not always picked the best settings.

Very nice! During our practical course we once used the ColorfulCell vector for a similar purpose www.addgene.org/62449/

Vimentin-mSG FRAP

You an even go as far as to color-code each cell-level datapoint by the experimental run it came from, so the reader can see if there if the cell variability is clustered or dispersed.

ER-lysosome contact sites (green) in MEFs on the background of an ER stain (grey, GFP-VAPA)