Excited to welcome ‪@adrienleger.bsky.social from‪ @nanoporetech.com to our RNA Symposium! 🎤 His talk will focus on how nanopore direct RNA sequencing can reveal multiple RNA modifications at once. Secure your spot at our symposium now 👉 zurl.co/mrhKq #RNA #ribosome #nanopore

Our paper using Oxford #Nanopore direct RNA sequencing to identify m6A modifications on RNA isoforms in human brain is now out in Science Advances. 🧪 www.science.org/doi/10.1126/...

🚨 We’re hiring ! 🚨 Join our cutting-edge research team as a molecular biologist at @nanoporetech.com HQ in Oxford. Perfect for a fresh PhD or MSc with a couple of years’ experience. Work at the interface of chemistry, molecular/synthetic biology and AI 👉 ejnh.fa.em2.oraclecloud.com/hcmUI/Candid...

The world prefers its energy more in the form of electricity than anything else.

At London Calling, Rosemary Sinclair Dokos and Lakmal Jayasinghe shared how we’re doubling down on what matters: delivering a future-ready platform with proven performance across DNA, RNA, and now proteins. Click here to discover what’s new – and why it matters: nanoporetech.com/news/london-...

#NanoporeConf Lakmal Jayasinghe ONT is the *only* sequencing technology that detects RNA modifications directly. They're now up to 8 modifications that can be detected simultaneously. [This is something I think ONT should be shouting from the rooftops every day] RNA barcoding is in beta testing.

New HAC models are 1.4Q more accurate - a 25% error reduction

Big improvements in modification calling accuracy - 6mA increases from Q16 to Q24

Dorado v1.0.0 introduces dramatic improvement to modified base calling speed

Base calling obeys scaling laws in deep learning - a Q28 base caller using standard chemistry is now available on demand.

Oxford Nanopore sequencing will be ubiquitous for multiomics….and the next step is proteomics. First panel-based proteomics assays, before driving towards full protein sensing in the future. #nanoporeconf

More details on new RNA modified bases detectable with @nanoporetech.com sequencing. #NanoporeConf

Dorado 1.0 🚀release with new RNA modified base models for 2’Ome as well as improvement of all DNA and RNA modified base models including massive improvement in 6mA for open chromatin prediction! Also a lot more non-mods related features😆! #NanoporeConf github.com/nanoporetech...

Dorado 1.0 🚀release with new RNA modified base models for 2’Ome as well as improvement of all DNA and RNA modified base models including massive improvement in 6mA for open chromatin prediction! Also a lot more non-mods related features😆! #NanoporeConf github.com/nanoporetech...

With significant ML advances, Dorado & MinKNOW now power built-in accurate variant calling, default methylation detection & short fragment mode. Adaptive sampling & T2T assemblies are production-ready — enabling faster, richer, more meaningful insights.

Dorado is 1.0! 🎉 LC25 release adds v5.2 DNA/RNA models with a new suite of mod models that are higher accuracy, lower FP rates, and significantly faster. - Introducing dorado variant 👀 - HAC error rate is down over 25%. - New 2’Ome mod models - Hopper & Blackwell SUP speed ups #Nanoporeconf

Your speakers for London Calling 2025! #NanoporeConf

Really pleased that this paper is out - academic.oup.com/neuro-oncolo... thanks to all our collaborators far and wide who have helped and of course @nanoporetech.com

Excited for another London Calling! Incredible updates coming over the next two days! #NanoporeConf @nanoporetech.com

I am quite interesting in this Nm modification. As we detected Nm in rRNA as well as mRNA using Nanopore RNA002 version. www.cell.com/molecular-ce...

I expect Rosemary and Lakmal (the new R&D team leads) will reveal more than just all this - I am looking forward to the reports on Wednesday and looking forward to catching up with everyone nanopore on Friday!

Nanopore now can call 4 RNA modifications - m6A (I love how DNA and RNA have different writing conventions - molecular biologists, just don't go changing!) - m5C, pseudourodine, and inosine. This is all new stuff, and there quite a bit on truth sets, sensitivity/specificity to play out

The final, most exotic stuff is RNA - so called "direct RNA" where modifications of the RNA is the really exciting stuff (the sequence itself, including intron/exon structure is probably still done best via cDNA, "old school").

Basically molecular biologists can either use incorporation (eg, BrdU) or modification (eg, bacterial enzymes tethered to antibodies) or chemical modification (eg, radicals etc) and then the read out is the DNA (or RNA, see below).

Nanopore can read other mods as well - hydroxymethylation, the oxidation product of methylation which is only really present in neuronal tissue in vertebrates (watch this space for insights on this - we will be preprinting soon!) and bacterial DNA methylation, like 6mA.

This week is the gathering of the tribes around @nanoporetech.com, for their annual meeting, London Calling. It is a blend of tech announcements, product updates + scientific conference (posters and breakout talks). I am only there for the informatics day at the end sadly, but here are some thoughts

Live from London Calling 2025! Speakers, attendees and the Oxford Nanopore crew are starting to fill into Old Billingsgate! Ready for Gordon to kick things off for the 11th time! Make sure you keep your posts coming using: #nanoporeconf

We are only hours away from the regular Tech update from Oxford @nanoporetech.com (18.15 UK time on Wednesday)

Now available as preprint: The ENCODE 4 expanded registry of regulatory elements - 2.35M 🧍 human cCREs - 927k 🐭 mouse cCREs www.biorxiv.org/content/10.1... Led by @moorejille.bsky.social, this preprint summarizes data and analyses generated by hundreds of contributors across ENCODE 4

Direct RNA sequencing is featured as one of The Scientist 2024 Top 10 Innovations. Read to find out how the latest direct RNA #nanopore sequencing kit and flow cell has improved accuracy and output, enabling a more complete picture of the landscape of RNA Read: www.the-scientist.com/2024-top-10-...

🎄 New Dorado release (v0.9.0) – and it's big! 🚀 Dorado polish command (experimental) for improving draft assemblies – faster & more accurate than Medaka ⚡ Faster modified base calling models 🔧 Usability & accuracy improvements: PolyA, Barcoding, 6mA calling github.com/nanoporetech...

In #NextGenSequencing news, Oxford @nanoporetech.com has released a new version of their basecalling software with new functionalities. An explainer ow what the basecalling software is and what this new release brings for ONT users. github.com/nanoporetech...

github.com/nanoporetech... - Faster modified base models for DNA 4mC_5mC, 5mC_5hmC, 5mCG_5hmCG, and 6mA

Interested in a career in science or technology? This summer we’re offering hands-on experience alongside our talented teams with our 2025 internships. Click to see our availability here: nanoporetech.com/about/career...

Computational with a PhD in some quantiative science, and want to make the world a better place with AI in healthcare? Want to work with some of the most innovative AI groups in the life sciences? Want to work for Europe, across Cambridge and Heidelberg, living in the UK? Two more days to apply:

Yesterday, I presented our latest results mining single molecule m6A data at #multiomics2024: We see changes in m6A levels following CRISPR-cas13 knock-down of oncogenic lncRNAs that are independent of mRNA expression levels, revealing new dimensions of highly pertinent biological information

We have been busing working on models to detect all 2'Ome-RNA modified nucleotides on top of PseudoU, m6A, m5C and Inosine using @nanoporetech.com direct RNA sequencing. This is still very preliminary but here are a few examples of what it looks like on Human #rRNA prepared with standard lib prep 🤩

Abstract submission for London Calling 2025 is open! If you have something to share, why not submit an abstract for your chance to speak at the biggest nanopore sequencing conference of the year. Learn more: nanoporetech.com/about/events... #nanoporeconf

Now that is #epigenetic at scale. I am very exited to see that this partneship with the @ukbiobank.bsky.social will be able to generate SOTA 5mC and 5hmC information with our latest chemistry and mod calling tools 💪

Since Oxford Nanopore sequencing does not require any chemical (bisulfite) conversion to detect methylation. It would mean long-range epigenetic modifications, structural variants (SVs),single nucleotide polymorphisms (SNPs), and repeats can all be identified and phased in a single dataset

Excited to join the #Nanopore Community on BlueSky. Follow us for the latest updates and discussions about Oxford Nanopore and Nanopore Community. #WYMM

I have made a new release of kyber, a tool to make a quick sketch of your long-read sequencing data or to compare multiple datasets. It uses a fixed grid of accuracy and read length. Now also supports ubam - feedback appreciated! github.com/wdecoster/ky...

The 1st rule of day science is to ask for each result, what is the negative control and what is the positive control, because we forget how easy it is to fool ourselves.

SPECIAL ISSUE! This month Genome Research publishes a diverse collection of research and review articles in a special issue highlighting advances in long-read sequencing applications in biology and medicine. Full issue links here: tinyurl.com/Genome-Res-3....

Despite a massive head start, BlueSky has now overtaken Threads in the US 👇

New preprint on our method aa-tRNA-seq: chemically capturing aminoacylated tRNA molecules & pulling them through nanopores. It’s been a blast working with my collaborator Aleks Radakovic to take this from idea to promising pilot data to some looking exciting biology to chase down in future work.

We strongly suggest that academic publishers and other platforms that host research rapidly implement a Share to Bluesky button for their articles. Here's how: docs.bsky.app/docs/advance... #AcademicSky #HigherEd #Altmetrics

Its been a crisp winter's day here in a London, with a patch blue sky and leads me to my first thread on Bluesky ... as followers who know me on the other site I consistently rage against co-opting science, in particular genetics, for new takes on racism

It's Christmas in advance with a very special @nanopore.bsky.social #opendata release ! 🎅 We are releasing our 5mC 5hmC 6mA DNA mod synthetic control datasets with each mods in all possible 5 mers contexts. All nicely wrapped in a nice validation blog post. labs.epi2me.io/mod-validati...

Interested in RNA modifications? Don't know where they are and worried about the chemical diversity of all the possibilities. Using clever molecular biology and the excellent @nanopore.bsky.social (not sure they monitor this) we have a solution! www.biorxiv.org/content/10.1...