Mirian Krystel De Siqueira (@desiqueiramk.bsky.social)

Protocol to perform polysome profiling in primary differentiating murine adipocytes Here, we present a protocol for polysome profiling in differentiating adipocytes from the mouse stromal vascular fraction. We describe steps for lysate preparation, ultracentrifugation through sucrose gradients, mRNA isolation, RNA sequencing, and motif enrichment. This protocol enables the analysis of actively translating mRNAs and translational gene regulation by isolating polysome-bound mRNA, revealing insights into protein synthesis during adipogenesis or stimuli responses. Applications incl... http://dlvr.it/TKVSXF