The plasmids associated with this paper are available on addgene now! www.addgene.org/browse/artic...

Welcome to the JCC family!

Our paper is finally out in Molecular Cell! 🚀 We uncover why PAM-relaxed Cas9 variants like SpRY are inefficient — they get kinetically trapped during target engagement. Mechanistic insights like this are key to engineering smarter, faster genome editors. Huge thanks to the team! #CRISPR #editing🔬🧬

Now online at Molecular Cell! What makes SpyCas9 such an efficient editor? Read more at: www.cell.com/molecular-ce... Congrats @honglue.bsky.social, Noor Al-Sayyad, and @kevinwasko.bsky.social!

“What we’ve done here is uncover the secret sauce that makes typical #Cas9 — the original Nobel molecule — so efficient and precise." – Co-first author @honglue.bsky.social of the @doudna-lab.bsky.social on his new paper out today in Molecular Cell Read here: shorturl.at/p664D

See details in this website gess.hms.harvard.edu/event/meirui...

🧪🧪🧪10 incredible findings about Cas10-relative, mCpol: result number 10 will surprise you!🧪🧪🧪 @erinedoherty.bsky.social and I teamed up to understand the role and function of Cas10-relative, mCpol, and its role in antiphage immunity. For more, check out Erin's thread 👇

Catch @honglue.bsky.social at the Harvard Genome Engineering Seminar Series this Monday, April 7 at 1pm EST / 10am PST! If you’ve ever wondered why SpyCas9 is so efficient at genome editing, don’t miss his talk—Zoom link: harvard.zoom.us/j/94394339529

Giving a seminar for the Genome Engineering Seminar Series at Harvard this Monday, April 7 at 1pm EST / 10am PST! 🎙️ I’ll be talking about our Cas9 work—if you’ve ever wondered what the recipe is for high-efficiency CRISPR genome editing, come hang out!🧬💥 📍 Zoom link: harvard.zoom.us/j/94394339529

Preprint alert! ✨ In this project that I co-led with @benadler.bsky.social, we show that a miniature CRISPR-Cas10-like enzyme, mCpol, uses a novel inverse signaling mechanism to prevent the spread of viruses that attempt immune evasion by depleting host cyclic nucleotides. Check it out:

Thanks to out @ucberkeleyofficial.bsky.social community for joining us at Stand Up For Science! @jenniferdoudna.bsky.social spoke about how NIH funding supported her PhD & NSF funding supported her development of #CRISPR genome editing. Federal funding is needed for life-saving science!