Leslie Day, PhD
@lesliedaymicro.bsky.social
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Anaerobic Microbiologist Damon Runyon Fellow Postdoc @ O’Toole Lab, Dartmouth
reposted by
Leslie Day, PhD
Gregory A. Wilson
about 22 hours ago
#ArvCon2026
to benefit
@damonrunyon.org
begins with our Pre-Show TOMORROW, Thursday the 21st at 7:30 p.m. ET...please mark your calendars for an event full of community, giveaways, surprises, interviews, music, and--of course--lots of TTRPGs! Please spread the word, and we'll see you tomorrow night!
add a skeleton here at some point
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Who needs a sauna when you can work in an anaerobic chamber on a warm summer day before the AC has caught up
2 days ago
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reposted by
Leslie Day, PhD
Gregory A. Wilson
6 days ago
Finally, Day Four of
#ArvCon2026
wraps up at 3:15 p.m. EDT with a special one-shot TTRPG adventure in the Gumshoe system, featuring
@damonrunyon.org
scientists
@lesliedaymicro.bsky.social
,
@txrwang.bsky.social
, and Hui Chiu as players, and
@therisingtithes.bsky.social
as GM! 11/?
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ArvanEleron - Twitch
I'm Arvan Eleron, a writer and gamer. My favorite games are RPGs, particularly tabletop D&D, but I'm also a sucker for a good adventure or turn based strategy game (and I love Gran Turismo, if I can e...
https://www.twitch.tv/arvaneleron
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reposted by
Leslie Day, PhD
Gregory A. Wilson
6 days ago
Day Two of
#ArvCon2026
begins on May 23rd at 12 p.m. EDT with an interview of amazing
@damonrunyon.org
scientists
@lesliedaymicro.bsky.social
,
@txrwang.bsky.social
, and Hui Chiu! 4/?
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ArvanEleron - Twitch
I'm Arvan Eleron, a writer and gamer. My favorite games are RPGs, particularly tabletop D&D, but I'm also a sucker for a good adventure or turn based strategy game (and I love Gran Turismo, if I can e...
https://www.twitch.tv/arvaneleron
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reposted by
Leslie Day, PhD
Evgenii Protasov
11 days ago
The extreme halophilic archaeon Haloferax volcanii reduced Fe(III) to conserve energy from extracellular electron transfer
#microbiology
#archaea
#Haloferax
#MicroSky
#ArchaeaSky
@asm.org
journals.asm.org/doi/10.1128/...
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reposted by
Leslie Day, PhD
Olivier Borkowski
15 days ago
Breathing both ways: simultaneous aerobic–anaerobic respiration in microbes
doi.org/10.1016/j.ti...
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reposted by
Leslie Day, PhD
Gordon Hodson
21 days ago
#AcademicSky
#Stats
Sometimes, statistical/methods concepts are best captured in an image. 👇
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reposted by
Leslie Day, PhD
Richard Lenski
20 days ago
The Barrick & Lenski Labs in Dept of Microbiology, Genetics, & Immunology at Michigan State Univ seek a Research Assistant II to manage their research operations and participate in projects related to microbial evolution and synthetic biology. See details below.
careers.msu.edu/jobs/researc...
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Research Assistant II - East Lansing, Michigan, United States
Position Summary The Barrick and Lenski Labs in the Department of Microbiology, Genetics, & Immunology at Michigan State University are seeking a Research Assistant II to manage their research ope...
https://careers.msu.edu/jobs/research-assistant-ii-east-lansing-michigan-united-states-8725dfd6-64b7-402d-b34a-daac3a7d968d
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reposted by
Leslie Day, PhD
MichaelMcMurray lab
29 days ago
Doing the periodic cleaning of used plasmid miniprep columns and realized in honor of Earth Day it may be worth reminding you molecular biologists: Plasmid miniprep columns can be cleaned and reused essentially indefinitely! Just add 1 M HCl, soak overnight and wash 3x w/ water. Save $ and plastic
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reposted by
Leslie Day, PhD
Buzz Baum
about 1 month ago
🎉 This is also a symbolic moment for the field: around 50 years since Carl Woese and colleagues first recognised Archaea as a distinct domain of life, fundamentally changing our understanding of evolution.
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reposted by
Leslie Day, PhD
Raphael Laurenceau
about 1 month ago
🔈Paper out! We turned the most fascinating phage host-switch mechanism, diversity-generating retroelements, into a programmable mutagenesis tool, DGRec. You can perform targeted hypermutation of any 50-200bp sequence directly in vivo in E. coli
www.nature.com/articles/s41...
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Diversity-generating retroelements for programmable targeted hypermutagenesis - Nature Biotechnology
Diversity-generating retroelements are engineered for directed evolution in E.coli.
https://www.nature.com/articles/s41587-026-03078-4
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reposted by
Leslie Day, PhD
Cameron Thrash
about 1 month ago
Breathing both ways: simultaneous aerobic–anaerobic respiration in microbes
www.cell.com/trends/micro...
#jcampubs
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Breathing both ways: simultaneous aerobic–anaerobic respiration in microbes
Microorganisms have historically been classified as obligate aerobes or anaerobes, facultative anaerobes, or microaerophiles, reflecting differences in respiratory strategies dictated by the use of ox...
https://www.cell.com/trends/microbiology/abstract/S0966-842X(26)00069-7?rss=yes
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reposted by
Leslie Day, PhD
Evgenii Protasov
about 1 month ago
Prokaryota Locomota 🪄! No,
#microbes
don't move by magic 😃. There are a number of ways and gliding motility is just one of them. Read more in a review from Journal of Bacteriology 🤓
#microbiology
#bacteria
#MicroSky
@asm.org
doi.org/10.1128/jb.0...
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
3 months ago
Our newest paper by Sarvesh Surve, a PhD student in the lab, explores the culturome of the regions of the gut for persons with CF. We see clear differences from healthy controls, esp. in the mucosal samples.
journals.asm.org/doi/10.1128/...
@asm.org
#JBacteriology
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
3 months ago
Excited about a recent paper from Bassam el Hafi, a recently graduated PhD student, & Fabrice Jean Pierre leveraging 4,200 respiratory microbiomes from CF (pre- and post-ETI), and testing one of the hypotheses that arose from this analysis.
journals.asm.org/doi/10.1128/...
@asm.org
#JBacteriology
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reposted by
Leslie Day, PhD
Damon Runyon Cancer Research Foundation
4 months ago
Major breakthroughs in cancer research can come from the most unexpected sources. This World Cancer Day, we celebrate the curiosity that drives our scientists to look for answers in unusual places.
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What does that have to do with cancer?
Current and former Damon Runyon scientists explain why breakthroughs can come from unusual places, and why Damon Runyon's funding strategy is so important.…
https://vimeo.com/1142182992?share=copy&fl=sv&fe=ci
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
4 months ago
A must read! The constant and often irrational renaming of microbes is out of control, and bad for microbiology and microbiologists.
journals.asm.org/doi/10.1128/...
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The disputed identity of Pseudomonas putida KT2440: when taxonomists rename your favorite microbe | mBio
Names matter; they are not mere labels. They operate as anchors of memory, social coordinates, and references of collective identity. When a name becomes established through long-term use and broad ad...
https://journals.asm.org/doi/10.1128/mbio.03390-25
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
4 months ago
The Microbial Diversity courseis top-notch! Hoping to see you there!
www.mbl.edu/education/ad...
add a skeleton here at some point
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reposted by
Leslie Day, PhD
Damon Runyon Cancer Research Foundation
5 months ago
No one organization can fill the gaps created by federal funding cuts to research. But as an independent funder, Damon Runyon is committed to ensuring that our scientists can continue their lifesaving work in 2026 and beyond. Until midnight, all gifts will be fully matched:
damonrunyon.org/donate
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Damon Runyon scientists on the impact of slashing research funding
Damon Runyon scientists explain how drastic cuts in federal research funding threaten progress in cancer research, and how Damon Runyon can help protect the breakthroughs…
https://vimeo.com/1147354275?share=copy&fl=sv&fe=ci
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reposted by
Leslie Day, PhD
The ADA Forsyth Institute
5 months ago
New from AFI researchers Jennifer Gundrum and Drs. Tabita Ramirez-Puebla, Jessica L. Mark Welch, and Gary G. Borisy: Novel FISH method overcomes cell wall barriers, enabling clearer insight into complex microbial communities. Learn more:
journals.asm.org/doi/10.1128/...
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reposted by
Leslie Day, PhD
ASM
5 months ago
The 2025 Agar Art Contest winners have been selected! This year’s theme, “Microbes Make the World Go Round,” inspired 557 global entries—from food fermentation to antibiotic production. See the masterpieces and finalists!
asm.org/press-releas...
#agarart
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@archaeabio.bsky.social
can maybe help here?
add a skeleton here at some point
5 months ago
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reposted by
Leslie Day, PhD
Evgenii Protasov
6 months ago
The latest and most detailed review on a fundamental biochemical pathway:
#glycolysis
#microbiology
#biochemistry
onlinelibrary.wiley.com/doi/full/10....
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
6 months ago
In a manuscript on BioRxiv, we describe experiments exploring the culturome of the gut of adults with CF and in health, revealing an interesting redistribution of the microbes in the lumen vs. mucosa. The study is led by Sarvesh Surve a PhD student in my lab.
www.biorxiv.org/content/10.1...
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reposted by
Leslie Day, PhD
Cameron Thrash
6 months ago
Rare Phyla, Such as CPR and DPANN, Shape Ecosystem-Level Microbial Community Structure Dissimilarities
link.springer.com/article/10.1...
#jcampubs
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Rare Phyla, Such as CPR and DPANN, Shape Ecosystem-Level Microbial Community Structure Dissimilarities - Microbial Ecology
Rare microbial lineages, such as members of the candidate phyla radiation (CPR) bacteria and Diapherotrites, Parvarchaeota, Aenigmarchaeota, Nanoarchaeota, and Nanohaloarchaeota (DPANN) archaea, are i...
https://link.springer.com/article/10.1007/s00248-025-02595-0
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When life gives you lab electrical shutdowns for maintenance … you make the most of the first bits of east coast snow!
6 months ago
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reposted by
Leslie Day, PhD
Robin A. Herbert
6 months ago
Very excited to share a big part of my dissertation work with the Deutschbauer lab at LBNL and
@ucberkeleyofficial.bsky.social
! BarTn7: A method for bacterial lineage tracking at sub-species resolution in population, ecological, and evolutionary experiments.
www.biorxiv.org/content/10.1...
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BarTn7: Optimizing Bacterial Lineage Tracking at Sub-Species Resolution for Population Dynamics in Ecological and Evolutionary Studies
Communities of bacteria undergo population bottlenecks which are crucial to their population, ecological, and evolutionary dynamics. However, conventional amplicon sequencing cannot distinguish such d...
https://www.biorxiv.org/content/10.1101/2025.11.24.690285v1
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
6 months ago
Excited to share new work from Sarvesh Surve examining the gut microbiota of adults with CF using colonoscopy samples and a culturomics approach. We note continued depletion of Bacteroidota (which we noted in kids) & enrichment of aerobes in the mucosa of pwCF.
pubmed.ncbi.nlm.nih.gov/41256617/
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reposted by
Leslie Day, PhD
Smart Alyk
6 months ago
DAY 168 SPECIES 168 - Prevotella intermedia STAGE: Bacteria MEDIUM: Arches Paper. Chromatek & HWC Watercolor. Sakura pen Thanks to
@lesliedaymicro.bsky.social
for suggesting it!
#sciart
#watercolor
#bacteria
#painting
#art
#paint
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reposted by
Leslie Day, PhD
Roland Hatzenpichler
6 months ago
Clone-FISH paper out: Manuscript/resource alert
#microsky
🦠 We present a collection of 30 E. coli (CloneFISH) cultures, each carrying a plasmid for the heterologous expression of a (near) full-length 16S rRNA gene from one of 30 lineages of archaea, including 17 yet uncultured ones.
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reposted by
Leslie Day, PhD
Jonathan Eisen
6 months ago
Science is awesome ...
www.cell.com/trends/biote...
#violacein
#pigments
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One-pot production of colored bacterial cellulose
Zhou et al. report the one-pot production of colored bacterial cellulose (BC) as a sustainable alternative to petroleum-based fabrics. Metabolic engineering enhanced colorant yields, while co-culture ...
https://www.cell.com/trends/biotechnology/fulltext/S0167-7799(25)00407-X
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reposted by
Leslie Day, PhD
Evgenii Protasov
6 months ago
Assembly, architecture and functional roles of microbial surface layers
#microbiology
#archaea
#bacteria
@natrevmicro.nature.com
www.nature.com/articles/s41...
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Assembly, architecture and functional roles of microbial surface layers - Nature Reviews Microbiology
Surface layers (S-layers) are ubiquitous protein assemblies that coat prokaryotic cells, with their functional roles increasingly coming into focus. In this Review, Isbilir and colleagues discuss rece...
https://www.nature.com/articles/s41579-025-01258-8
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reposted by
Leslie Day, PhD
Cameron Thrash
7 months ago
Increased mutation rates and diversity are dominant features of Geobacter multiheme cytochromes
www.biorxiv.org/content/10.1...
#jcampubs
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reposted by
Leslie Day, PhD
Fabrice Jean-Pierre
7 months ago
"Functional CFTR may be required for Prevotella melaninogenica regulation of epithelial cell defense against Staphylococcus aureus"
pubmed.ncbi.nlm.nih.gov/41198444/
#microsky
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Functional CFTR may be required for Prevotella melaninogenica regulation of epithelial cell defense against Staphylococcus aureus - PubMed
P. melaninogenica enhanced neutrophil and epithelial defense against S. aureus, but the benefits of epithelial cell regulation by P. melaninogenica were lost with CFTR dysfunction. CFTR modulators rescued P. melaninogenica responsiveness in epithelial cells, highlighting the potential for synergisti …
https://pubmed.ncbi.nlm.nih.gov/41198444/
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Prevotella is the clear favorite of the 4-member cookie community (not like I’m surprised)
@bassamhafi.bsky.social
@fabricejpierre.bsky.social
7 months ago
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
7 months ago
Congratulations to Bassam el Hafi, who successfully defended his PhD thesis yesterday! He did a terrific job! Bassam connected microbial interactions to the complexity of human communication. Thanks also to Sarah Clark of the Anschutz School of Medicine who served at Bassam's outside examiner.
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
7 months ago
New in JB: A minireview from my team, led by a recently graduated PhD student Kaitlyn Barrack, compares the microbiota and physiology of the CF gut with features of the gut in inflammatory bowel disease - there are many similarities!
journals.asm.org/doi/10.1128/...
@asm.org
#JBacteriology
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reposted by
Leslie Day, PhD
Yunha Hwang
7 months ago
We're thrilled to announce SeqHub, an AI-enabled platform for biological sequence analysis. SeqHub brings together sequence search, genome annotation, and data sharing in one place.
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
8 months ago
Excited to be part of this collaboration with the Burrows lab exploring twitching and surface surface sensing by P. aeruginosa. This work further delineates a role for the T4P motor proteins and pili for surface sensing.
journals.asm.org/doi/10.1128/...
@asm.org
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reposted by
Leslie Day, PhD
Damon Runyon Cancer Research Foundation
8 months ago
Earlier this month, we proudly welcomed the newest cohort of Damon Runyon Fellows! These 16 brilliant postdocs will receive four years of independent funding to advance their investigation of causes, treatments, and prevention strategies for all types of cancer. Read more:
https://bit.ly/4nc25Hq
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reposted by
Leslie Day, PhD
ASM
8 months ago
In
#mBio
, dive into story behind the 1989 discovery of Helicobacter pylori CagA, the first tumorigenic bacterial protein. This article recounts the early steps in the investigative process and relates some of the unexpected discoveries that ensued.
asm.social/2C1
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reposted by
Leslie Day, PhD
Fabrice Jean-Pierre
8 months ago
Happy to finally see this out! Bassam El Halfi (
@bassamhafi.bsky.social
- my first rotation student when I started my post-doc in
@geiselbiofilm.bsky.social
) did an amazing job investigating mechanisms of interspecies metabolic interactions. Check it out!
#microsky
journals.asm.org/doi/10.1128/...
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Pseudomonas aeruginosa supports the survival of Prevotella melaninogenica in a cystic fibrosis lung polymicrobial community through metabolic cross-feeding | mBio
Polymicrobial interactions impact disease outcomes in pwCF who suffer from chronic respiratory infections. Previous work established a CF-relevant polymicrobial community model that allows experimenta...
https://journals.asm.org/doi/10.1128/mbio.01594-25
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
9 months ago
Excited to share our latest work, driven by my recently graduated PhD student Kaitlyn Barrack, exploring the mechanisms of bacterial dysbiosis in the CF gut - including a role for a colorectal cancer-associated genotoxin in this competition.
www.biorxiv.org/content/10.1...
@kbarrack.bsky.social
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
9 months ago
Excited about our most recent collaboration with the Wong lab, lead by William Schmidt, describing a T4P and CdrA-mediated mechanism of sensing surface EPS. Fun stuff and great work William and team.
www.nature.com/articles/s41...
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reposted by
Leslie Day, PhD
John Spear
9 months ago
The latest and greatest from an amazing team of authors. See what you think: A Comprehensive Framework for Assessing Terrestrial Analogue Field Sites for Ocean Worlds - Stern - 2025 - Journal of Geophysical Research: Planets - Wiley Online Library
agupubs.onlinelibrary.wiley.com/doi/10.1029/...
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A Comprehensive Framework for Assessing Terrestrial Analogue Field Sites for Ocean Worlds
The choice of analogue field site for ocean worlds research requires traceability to a well-developed science question We present a two-step process to evaluate field sites with respect to their ...
https://agupubs.onlinelibrary.wiley.com/doi/10.1029/2024JE008803
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Incredibly honored to be a Damon Runyon Fellow!
@damonrunyon.org
add a skeleton here at some point
9 months ago
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
9 months ago
Our newest manuscript on the gut-lung axis in CF, lead by Rebecca Valls, explores sex and intestinal regional differences in response to Bacteroides. Check it out!
www.biorxiv.org/content/10.1...
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
9 months ago
We had the O'Toole Lab Reunion on Sat here at Dartmouth, celebrating 26 amazing years - science is all about the people, and I have been fortunate to have a great bunch of folks in the lab over the years! And THANKS to all the lab members who did all the organizing!
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reposted by
Leslie Day, PhD
ASM
9 months ago
Microbes rarely exist alone; instead, they live in dynamic communities. In
#IAIJournal
, researchers discuss the interplay between the host environment & microbiome metabolism & the impacts on disease progression in host-associated polymicrobial communities.
asm.social/2yf
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
9 months ago
Excited about our most recent paper lead by Kaitlyn Barrack, a minireview in JB comparing the CF and IBD guts - from microbiomes to physiologies. There are many similarities...
journals.asm.org/doi/10.1128/...
@asm.org
#JBacteriology
@kbarrack.bsky.social
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