Leslie Day, PhD
@lesliedaymicro.bsky.social
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Anaerobic Microbiologist Damon Runyon Fellow Postdoc @ O’Toole Lab, Dartmouth
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Leslie Day, PhD
Damon Runyon Cancer Research Foundation
2 days ago
No one organization can fill the gaps created by federal funding cuts to research. But as an independent funder, Damon Runyon is committed to ensuring that our scientists can continue their lifesaving work in 2026 and beyond. Until midnight, all gifts will be fully matched:
damonrunyon.org/donate
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Damon Runyon scientists on the impact of slashing research funding
Damon Runyon scientists explain how drastic cuts in federal research funding threaten progress in cancer research, and how Damon Runyon can help protect the breakthroughs…
https://vimeo.com/1147354275?share=copy&fl=sv&fe=ci
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Leslie Day, PhD
The ADA Forsyth Institute
11 days ago
New from AFI researchers Jennifer Gundrum and Drs. Tabita Ramirez-Puebla, Jessica L. Mark Welch, and Gary G. Borisy: Novel FISH method overcomes cell wall barriers, enabling clearer insight into complex microbial communities. Learn more:
journals.asm.org/doi/10.1128/...
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Leslie Day, PhD
ASM
19 days ago
The 2025 Agar Art Contest winners have been selected! This year’s theme, “Microbes Make the World Go Round,” inspired 557 global entries—from food fermentation to antibiotic production. See the masterpieces and finalists!
asm.org/press-releas...
#agarart
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@archaeabio.bsky.social
can maybe help here?
add a skeleton here at some point
25 days ago
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Leslie Day, PhD
Evgenii Protasov
27 days ago
The latest and most detailed review on a fundamental biochemical pathway:
#glycolysis
#microbiology
#biochemistry
onlinelibrary.wiley.com/doi/full/10....
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
about 1 month ago
In a manuscript on BioRxiv, we describe experiments exploring the culturome of the gut of adults with CF and in health, revealing an interesting redistribution of the microbes in the lumen vs. mucosa. The study is led by Sarvesh Surve a PhD student in my lab.
www.biorxiv.org/content/10.1...
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Leslie Day, PhD
Cameron Thrash
about 1 month ago
Rare Phyla, Such as CPR and DPANN, Shape Ecosystem-Level Microbial Community Structure Dissimilarities
link.springer.com/article/10.1...
#jcampubs
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Rare Phyla, Such as CPR and DPANN, Shape Ecosystem-Level Microbial Community Structure Dissimilarities - Microbial Ecology
Rare microbial lineages, such as members of the candidate phyla radiation (CPR) bacteria and Diapherotrites, Parvarchaeota, Aenigmarchaeota, Nanoarchaeota, and Nanohaloarchaeota (DPANN) archaea, are i...
https://link.springer.com/article/10.1007/s00248-025-02595-0
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When life gives you lab electrical shutdowns for maintenance … you make the most of the first bits of east coast snow!
about 1 month ago
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reposted by
Leslie Day, PhD
Robin A. Herbert
about 1 month ago
Very excited to share a big part of my dissertation work with the Deutschbauer lab at LBNL and
@ucberkeleyofficial.bsky.social
! BarTn7: A method for bacterial lineage tracking at sub-species resolution in population, ecological, and evolutionary experiments.
www.biorxiv.org/content/10.1...
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BarTn7: Optimizing Bacterial Lineage Tracking at Sub-Species Resolution for Population Dynamics in Ecological and Evolutionary Studies
Communities of bacteria undergo population bottlenecks which are crucial to their population, ecological, and evolutionary dynamics. However, conventional amplicon sequencing cannot distinguish such d...
https://www.biorxiv.org/content/10.1101/2025.11.24.690285v1
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
about 1 month ago
Excited to share new work from Sarvesh Surve examining the gut microbiota of adults with CF using colonoscopy samples and a culturomics approach. We note continued depletion of Bacteroidota (which we noted in kids) & enrichment of aerobes in the mucosa of pwCF.
pubmed.ncbi.nlm.nih.gov/41256617/
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reposted by
Leslie Day, PhD
Smart Alyk
about 1 month ago
DAY 168 SPECIES 168 - Prevotella intermedia STAGE: Bacteria MEDIUM: Arches Paper. Chromatek & HWC Watercolor. Sakura pen Thanks to
@lesliedaymicro.bsky.social
for suggesting it!
#sciart
#watercolor
#bacteria
#painting
#art
#paint
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reposted by
Leslie Day, PhD
Roland Hatzenpichler
about 2 months ago
Clone-FISH paper out: Manuscript/resource alert
#microsky
🦠 We present a collection of 30 E. coli (CloneFISH) cultures, each carrying a plasmid for the heterologous expression of a (near) full-length 16S rRNA gene from one of 30 lineages of archaea, including 17 yet uncultured ones.
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reposted by
Leslie Day, PhD
Jonathan Eisen
about 2 months ago
Science is awesome ...
www.cell.com/trends/biote...
#violacein
#pigments
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One-pot production of colored bacterial cellulose
Zhou et al. report the one-pot production of colored bacterial cellulose (BC) as a sustainable alternative to petroleum-based fabrics. Metabolic engineering enhanced colorant yields, while co-culture ...
https://www.cell.com/trends/biotechnology/fulltext/S0167-7799(25)00407-X
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reposted by
Leslie Day, PhD
Evgenii Protasov
about 2 months ago
Assembly, architecture and functional roles of microbial surface layers
#microbiology
#archaea
#bacteria
@natrevmicro.nature.com
www.nature.com/articles/s41...
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Assembly, architecture and functional roles of microbial surface layers - Nature Reviews Microbiology
Surface layers (S-layers) are ubiquitous protein assemblies that coat prokaryotic cells, with their functional roles increasingly coming into focus. In this Review, Isbilir and colleagues discuss rece...
https://www.nature.com/articles/s41579-025-01258-8
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reposted by
Leslie Day, PhD
Cameron Thrash
about 2 months ago
Increased mutation rates and diversity are dominant features of Geobacter multiheme cytochromes
www.biorxiv.org/content/10.1...
#jcampubs
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reposted by
Leslie Day, PhD
Fabrice Jean-Pierre
about 2 months ago
"Functional CFTR may be required for Prevotella melaninogenica regulation of epithelial cell defense against Staphylococcus aureus"
pubmed.ncbi.nlm.nih.gov/41198444/
#microsky
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Functional CFTR may be required for Prevotella melaninogenica regulation of epithelial cell defense against Staphylococcus aureus - PubMed
P. melaninogenica enhanced neutrophil and epithelial defense against S. aureus, but the benefits of epithelial cell regulation by P. melaninogenica were lost with CFTR dysfunction. CFTR modulators rescued P. melaninogenica responsiveness in epithelial cells, highlighting the potential for synergisti …
https://pubmed.ncbi.nlm.nih.gov/41198444/
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Prevotella is the clear favorite of the 4-member cookie community (not like I’m surprised)
@bassamhafi.bsky.social
@fabricejpierre.bsky.social
about 2 months ago
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
about 2 months ago
Congratulations to Bassam el Hafi, who successfully defended his PhD thesis yesterday! He did a terrific job! Bassam connected microbial interactions to the complexity of human communication. Thanks also to Sarah Clark of the Anschutz School of Medicine who served at Bassam's outside examiner.
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
2 months ago
New in JB: A minireview from my team, led by a recently graduated PhD student Kaitlyn Barrack, compares the microbiota and physiology of the CF gut with features of the gut in inflammatory bowel disease - there are many similarities!
journals.asm.org/doi/10.1128/...
@asm.org
#JBacteriology
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reposted by
Leslie Day, PhD
Yunha Hwang
2 months ago
We're thrilled to announce SeqHub, an AI-enabled platform for biological sequence analysis. SeqHub brings together sequence search, genome annotation, and data sharing in one place.
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
3 months ago
Excited to be part of this collaboration with the Burrows lab exploring twitching and surface surface sensing by P. aeruginosa. This work further delineates a role for the T4P motor proteins and pili for surface sensing.
journals.asm.org/doi/10.1128/...
@asm.org
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reposted by
Leslie Day, PhD
Damon Runyon Cancer Research Foundation
4 months ago
Earlier this month, we proudly welcomed the newest cohort of Damon Runyon Fellows! These 16 brilliant postdocs will receive four years of independent funding to advance their investigation of causes, treatments, and prevention strategies for all types of cancer. Read more:
https://bit.ly/4nc25Hq
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reposted by
Leslie Day, PhD
ASM
3 months ago
In
#mBio
, dive into story behind the 1989 discovery of Helicobacter pylori CagA, the first tumorigenic bacterial protein. This article recounts the early steps in the investigative process and relates some of the unexpected discoveries that ensued.
asm.social/2C1
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reposted by
Leslie Day, PhD
Fabrice Jean-Pierre
4 months ago
Happy to finally see this out! Bassam El Halfi (
@bassamhafi.bsky.social
- my first rotation student when I started my post-doc in
@geiselbiofilm.bsky.social
) did an amazing job investigating mechanisms of interspecies metabolic interactions. Check it out!
#microsky
journals.asm.org/doi/10.1128/...
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Pseudomonas aeruginosa supports the survival of Prevotella melaninogenica in a cystic fibrosis lung polymicrobial community through metabolic cross-feeding | mBio
Polymicrobial interactions impact disease outcomes in pwCF who suffer from chronic respiratory infections. Previous work established a CF-relevant polymicrobial community model that allows experimenta...
https://journals.asm.org/doi/10.1128/mbio.01594-25
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
4 months ago
Excited to share our latest work, driven by my recently graduated PhD student Kaitlyn Barrack, exploring the mechanisms of bacterial dysbiosis in the CF gut - including a role for a colorectal cancer-associated genotoxin in this competition.
www.biorxiv.org/content/10.1...
@kbarrack.bsky.social
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
4 months ago
Excited about our most recent collaboration with the Wong lab, lead by William Schmidt, describing a T4P and CdrA-mediated mechanism of sensing surface EPS. Fun stuff and great work William and team.
www.nature.com/articles/s41...
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reposted by
Leslie Day, PhD
John Spear
4 months ago
The latest and greatest from an amazing team of authors. See what you think: A Comprehensive Framework for Assessing Terrestrial Analogue Field Sites for Ocean Worlds - Stern - 2025 - Journal of Geophysical Research: Planets - Wiley Online Library
agupubs.onlinelibrary.wiley.com/doi/10.1029/...
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A Comprehensive Framework for Assessing Terrestrial Analogue Field Sites for Ocean Worlds
The choice of analogue field site for ocean worlds research requires traceability to a well-developed science question We present a two-step process to evaluate field sites with respect to their ...
https://agupubs.onlinelibrary.wiley.com/doi/10.1029/2024JE008803
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Incredibly honored to be a Damon Runyon Fellow!
@damonrunyon.org
add a skeleton here at some point
4 months ago
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
4 months ago
Our newest manuscript on the gut-lung axis in CF, lead by Rebecca Valls, explores sex and intestinal regional differences in response to Bacteroides. Check it out!
www.biorxiv.org/content/10.1...
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
4 months ago
We had the O'Toole Lab Reunion on Sat here at Dartmouth, celebrating 26 amazing years - science is all about the people, and I have been fortunate to have a great bunch of folks in the lab over the years! And THANKS to all the lab members who did all the organizing!
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reposted by
Leslie Day, PhD
ASM
5 months ago
Microbes rarely exist alone; instead, they live in dynamic communities. In
#IAIJournal
, researchers discuss the interplay between the host environment & microbiome metabolism & the impacts on disease progression in host-associated polymicrobial communities.
asm.social/2yf
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
5 months ago
Excited about our most recent paper lead by Kaitlyn Barrack, a minireview in JB comparing the CF and IBD guts - from microbiomes to physiologies. There are many similarities...
journals.asm.org/doi/10.1128/...
@asm.org
#JBacteriology
@kbarrack.bsky.social
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reposted by
Leslie Day, PhD
National Biofilms Innovation Centre (NBIC)
5 months ago
✨Our annual
#BiofilmCreate
Competition is open for 2025! 🎨 📷 Showcase your talent in
#photography
/
#art
whilst highlighting the impact of
#biofilms
🏆Gift cards will be awarded in both categories 🗳️biofilms.ac.uk/create
#science
#scicomm
#AMR
#MicroSky
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Microbial Diversity 💜
5 months ago
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
5 months ago
JB Editor's Choice: Our latest in the Microbial Model Systems series (
journals.asm.org/topic/sss-ta...
) is "Milestones in the development of Myxococcus xanthus as a model multicellular bacterium" - from many contributors!
journals.asm.org/doi/10.1128/...
@asm.org
#JBacteriology
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@amnh.org
pleaaaseeeeee let me help update this! Everything else about the biodiversity room is amazing but this hurt my archaea loving soul.
5 months ago
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reposted by
Leslie Day, PhD
Fabrice Jean-Pierre
6 months ago
"Respiratory viral infection is associated with increased Pseudomonas abundance in cystic fibrosis airways"
www.biorxiv.org/content/10.1...
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Respiratory viral infection is associated with increased Pseudomonas abundance in cystic fibrosis airways
Acute respiratory viral infections are an important driver of morbidity and mortality in people with chronic lung disease and are frequently associated with pulmonary exacerbations and a transition fr...
https://www.biorxiv.org/content/10.1101/2025.07.08.663034v1.abstract
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Always a blast getting to lead a bunch of great scientists into a swamp to light methane on fire!
#volta
add a skeleton here at some point
6 months ago
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reposted by
Leslie Day, PhD
George O'Toole (he/him/his)
6 months ago
Last night at Microbial Diversity, we all went out for a MD tradition- the Volta experiment! A dirty but happy crew!
@mblscience.bsky.social
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Heading to one of my favorite places! Excited to share my anaerobe/genetics love & learn from the incredible people at the
@mblscience.bsky.social
and Microbial Diversity Course!
6 months ago
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reposted by
Leslie Day, PhD
Nature Microbiology
6 months ago
#NewResearch
🚨Out now! Structural analyses of the extracellular flagellum shows mechanism for flagellin incorporation
#MicroSky
🦠
www.nature.com/articles/s41...
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The structure of the complete extracellular bacterial flagellum reveals the mechanism of flagellin incorporation - Nature Microbiology
Cryo-electron microscopy analysis of the extracellular flagellum structure, including the hook–filament junction and filament cap complex, reveals mechanisms of flagellin incorporation into the growin...
https://www.nature.com/articles/s41564-025-02037-0?utm_source=bluesky&utm_medium=social&utm_campaign=nmicrobiol
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reposted by
Leslie Day, PhD
Nature Microbiology
6 months ago
#NewResearch
🚨Out now In situ sub-nanometre structure of the Campylobacter jejuni flagellar motor revealing the periplasmic scaffold and basis for torque generation
@beebylab.bsky.social
#MicroSky🦠🧫
www.nature.com/articles/s41...
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In situ structure of a bacterial flagellar motor at subnanometre resolution reveals adaptations for increased torque - Nature Microbiology
In situ cryoEM and modelling of the Campylobacter jejuni flagellar motor characterize the periplasmic scaffold and the structural basis for increased torque generation.
https://www.nature.com/articles/s41564-025-02012-9?utm_source=bluesky&utm_medium=social&utm_campaign=nmicrobiol
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reposted by
Leslie Day, PhD
Amy D Willis
6 months ago
We had a last minute cancellation for STAMPS -- a phenomenal course on microbiome data analysis. Woods Hole, MA, July 14-24 2025. If you or *anyone* you know is interested to attend, please email Titus &/or me... and we'll do what we can!!! Thx for sharing widely! 🤞❤️
add a skeleton here at some point
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reposted by
Leslie Day, PhD
Tami Lieberman
6 months ago
Our paper demonstrating that within-species warfare interactions are ecologically important on human skin is now published in Nature Micro!
www.nature.com/articles/s41...
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