More than a decade since CRISPR-Cas9 knock in came on the scene, it's remained underutilized in emerging models. Using transposase, and a split-FP system, we built an optimized knock in system for use in the genetically-enabled urchin, Lytechinus pictus. bit.ly/4wswLsd

I just learned a lab appropriate alternative to rock, paper, scissors- Kelp, Urchin, Rock. Kelp covers the rock, urchin eats the kelp, rock squashes the urchin!

Fantastic!

Uniform.

Happy #FluorescenceFriday! Today we're celebrating a crowd-favorite - Lamin B1! This cell line and plasmid illuminates the nuclear envelope. Our entire Allen Cell Collection is available for just the cost of shipping from @coriellinstitute.bsky.social and @addgene.bsky.social. #CellLineOfTheWeek

Is it just me or does everyone’s lab look at them like they sprouted a third eye every time they invoke Maniatis in the lab? I swear that we isolated good nucleic acids in the 90s and we didn’t have Reddit.

“If you have a broken radio and you have the circuit diagram, you’ll be in a better position to fix it.” - Nuno da Costa Thank you @b0yle.bsky.social for sharing our publication of the most complete map yet of the brain's wiring and activity in @geekwire.com! 🧠📈 www.geekwire.com/2025/brain-m...

Have you used our datasets, atlases, protocols, cell lines, and other #OpenScience resources in your research or classroom? Tell us about it! Share your story with us at bit.ly/opensciences... Select stories will be shared on our social media channels during #OpenScienceWeek.

Distinct requirements for PI3K isoforms p110α and p110δ for PIP3 synthesis in mouse oocytes and early embryos Read this Research Article by Anne Bourdais, Guillaume Halet and colleagues: doi.org/10.1242/dev.... 🎥KitL activates oocyte PI3K

EMBOMtg Paris May 6-9 @aydoganlab.bsky.social @jamesbriscoe.bsky.social @brunetlab.bsky.social @ebisuyamiki.bsky.social @mwdorr.bsky.social @beyerlab.bsky.social @franckp.bsky.social @janereznick.bsky.social @olmedo-lab.bsky.social @perez-carrasco.bsky.social meetings.embo.org/event/25-dev...

Nice work! Can’t wait to hear about this next week at DBSUMI.

This paper is now published at @diffjournal.bsky.social! Congratulations to all the hardworking students who made it happen! Shout out to authors 1 & 2! MJ is our “axolotl whisperer” and Raneesh is our computational wizard- both DVM/PhD students @ucdavisvetmed.bsky.social! 💫 doi.org/10.1016/j.di...

NOW HIRING - @adc-neuroenv.bsky.social is seeking a postdoc researcher to develop and optimize genetically encoded fluorescent and bioluminescent sensors tailored for marine model organisms. Please repost and share to your networks! 🔗 www.adc-nice.org/postdoctoral... @nathanshaner.bsky.social

101 L. pictus genes! 32hours. drive.google.com/drive/folder...

And... it's live! In situ hybridization has been foundational for sea urchin developmental biology. Here we report a fully automated method to probe more than a hundred genes using a general purpose liquid handler.https://www.biorxiv.org/content/10.1101/2025.03.23.644641v1

The "superpower" of sea urchins is the fact that a single female can produce millions of embryos. In the next day or so we will share a new method to analyze gene expression methods in these embryos using a fully automated, open platform.

A paywalled article from @nature.com bemoaning the exploitation of scientists' unpaid labour. Chef's kiss. Perfection. No notes.