A new era for #ONT amplicon sequencing? We show that #ONT #amplicon sequencing now achieves accuracy sufficient for #ASV resolution using standard Illumina-based pipelines. We validated this by sequencing identical amplicons on #ONT and #PacBio. @nanoporetech.com www.biorxiv.org/content/10.6...

The sun brings out the green @nanoporetech.com 🟩🟩🟩🟩🟩🚀🧬 Happy Friday everyone.

It is absolutely outrageous that Moderna’s flu vaccine was met with a “refusal-to-file” even after their approved their protocol with FDA and carried out the trial as agreed. This vaccine works better in older adults than the current flu vaccines. apnews.com/article/mode...

Hey @bsky.app can we get a poll option?

claude: I have no idea why we now have more unmapped reads than before after adding in mismatch scoring me: did you reverse complement the genome sequence? claude: i'm a dumbass

New hifiasm-ONT assembly method delivers high-quality, cost-efficient near T2T assemblies using standard Oxford Nanopore Simplex reads, broadening access to comprehensive genome assemblies across research and clinical applications. https://bit.ly/4awzU15

I think Single-M was written with that use-case in mind. Haven't tested it personally so can't vouch for it, but likely worth a try. www.nature.com/articles/s41...

interesting nanopore sequencing experience. found this in the middle of my genome assembly. driven by a single read with a big homopolymer in the middle of it? pretty weird, something to look out for i guess (i wonder how many of these are in Genbank...)

With the P2S joining the long list of abandoned @nanoporetech.com products in favour of the expensive P2i it becomes clearer that the #anyone #anywhere vision is lost...

I’m now at the wastewater symposium! Come say hi if you’re here and talk about high accuracy #longread sequencing for #microbial, #microbiome, and #metagenomics. @pacbio.bsky.social

I am calling for a complete and total boycott of the Mercator projection in all news stories about Greenland until every member of the American public has seen this

Been doing a lot of @nanoporetech.com sequencing for soil and sediment lately and it appears that no matter how much DNA or how little we still get decent yields. So pretty robust performance. Yield clearly depends on pore count.

Anyone gotten their hands on something crazy? Nvidia B200 or B300?

📢 20 postdoctoral positions (full-time, 4 years) for outstanding female scientists 👩‍🔬 🔬 @univie.ac.at The E-STEEM programme call opens January 7th, 2026 and closes March 3rd, 2026. careers.univie.ac.at/en/postdoc/e... I participate as a host...

The @nanoporetech.com 🎅 forgot the wrapping paper 🎄🎁

One flowcell from @nanoporetech.com yielded 260 Gbp 🎉🚀🤯🟩

Imagine sequencing the DNA of an entire country. Many colleagues have worked for years to sequence the DNA of soil and sediment samples covering Denmark to characterise the Danish microbial diversity 🧬🚀🎉🤯

Metagenomics colleagues! I'm looking for studies where both Illumina and ONT sequencing were performed on the same samples from soil, human, ruminent, and other sample types for comparison. Bonus if those studies include PacBio data. Please help and share!

Revio is a great product - it dramatically increased throughput over Sequel II (3x more data per cell, 12x more data per fully loaded run) and dropped the cost substantially. It's been the most successful instrument launch for the company, and now PacBio sequencing is at an all time high.

Beating our previous record by a wide margin I asked myself "Are @nanoporetech.com flowcells getting better?" 🚀🟩🧬 Let's find out! I created a form where you can register your yields so we can get a better overview. github.com/Kirk3gaard/O...

Did not expect to see disk space become an issue but after a disk clean up and restart I ended up with 243 and 244 gbp for these two @nanoporetech.com flowcells 🎉🚀🧬 💚🟩💚🟩💚🟩

After a day with almost 30 participants in the lab for our hands on @nanoporetech.com metagenomics course it is a blessing to see 🟩🟩🟩🟩🟩🟩🟩🟩🟩

@kirk3gaard.bsky.social , not recently active on Bluesky, on the 'tech bro' backing of Oxford @nanoporetech.com . We kind of already have a #techbro backing ONT, #LarryEllis who is one of the main investors and has put a ton of money into Oxford's EIT.

Please share this with anyone who may be interested in a post-doc in Germany: jobs.awi.de/Vacancies/20... This is quite an exciting opportunity to push the boundaries of what is known regarding the molecular basis of the formation and demise of photosymbiotic relationships in marine habitats.

Anyone else seeing @nanoporetech.com flowcells starting to "die" exactly around 72 hours? Could this be voltage control not being optimised for longer durations?

Preprint: Comparing BONCAT and MAR. Pro tips: don't buy the ClickIT kit, it's 25x overpriced as compared to buying individual chemicals by themselves (see my 2015 protocol chapter) and making reagents fresh gives superior results. And yes, only use picolyl dyes, my lab has been doing so since 2018.

How I like my @nanoporetech.com runs 🟩💚🟩🚀🧬

I'm recruiting a postdoc to figure out how aerobic methanotrophic bacteria oxidise methane in anoxic freshwater sediment environments: international.au.dk/about/profil... Come to Aarhus University in Denmark and work on this 2-year project funded by @villumfonden.bsky.social!

You load one @nanoporetech.com flowcell and it almost ruins your day 💩💩💩. You load six more and they look stunning 🟩💚🟩💚💚🟩

Still 10 days left to apply! 🦠🧪👩‍🎓🔬 Come work with my team in beautiful Vienna!

208Gb and can't tell you because it was for a customer @timdepooter.bsky.social

High yields are nice, but I think more important is highlighting the current robustness of the PromethION platform. In 2 weeks we processed 48 FC's resulting in 7.4Tb of data. That's over 150GB per FC on average. We definitely need to start thinking about mutliplexing. @nanoporetech.com

Professor (full) or Associate Professor (tenure track) in Microbial Genetics of Health (U. Turku Finland). Competitive starting package included! Please share. duunitori.fi/tyopaikat/ty...

193-200Gbp without any washing step (sewer biofilms & soil enrichments) ❇️🚀

Wow! I am very much against this. The deep value of GenBank is in it being a public domain database with 0 limitations on use. The norm that when we publish, we share the sequencing data supporting its claims *with 0 limitations on use* is so important for open science. I hope it doesn't change!

What is your highest yield on a @nanoporetech.com flowcell? And what was the sample type?

Ahh, have you seen this one? To me, this is the crown jewel of long read metagenomics at the moment? Gives some guidelines on how deep you need to sequence soil to get decent MAG recovery. www.nature.com/articles/s41...

Have not done much @nanoporetech.com sequencing lately but this looks pretty 🟩🟩🟩🟩🟩🟩 #MFDExtreme

Gotta catch em all! A little late night sampling for the MFD Extreme project. What microbes call this sand their home?

Beautiful evening for sampling. Microbes live in so many funky places and I finally got the chance to hunt them in locations that were not wastewater treatment plants 🎉🦠🧬🇩🇰 #MFDExtreme

Slowly it's sinking in 😍 Very excited about my new role as #Assistant #Professor @univie.ac.at @cemess.bsky.social! 🦠👩‍🎓🔬 It's great to be part of this fantastic team of friends and colleagues! 🧪 #Microsky

We have a new 3-year postdoc position in our group at the @hifmb.de to study plasmids and plasmids systems of the marine environment to survey their utility in microbial responses to environmental change. Please see the official job ad here, and spread the word: jobs.awi.de/Vacancies/20...

Can we please get Dorado 1.0.0+ in @nanoporetech.com minknow ASAP? 4090 also jumps quite a bit and is getting closer to support a full P2S with live sup 🎉

Got a chance to take the @nanoporetech.com hyp(er accuracy) model for a spin. Pretty decent improvement in read quality 🚀 but expect to spend quite some more GPU hours 😉 (Reads were mapped with minimap2 and identity scores from nanoplot were used for phred score calculations)

With all that is happening it is very exciting to see the Price Lab's very first, in-house, Oxford Nanopore direct RNA seq running! Not bad for a ~3 year expired flow cell!

Wandering while wondering if this sample cup will hold a story exciting enough for @isme-microbes.bsky.social conference in New Zealand next year 🦠

I tested myloasm on a 50x Klebsiella isolate, and it was very fast - only took about 1 minute to complete (on my Macbook).

Promising toolkit for binning and visualizing assembly graphs. The assembly graph is an underutilized source of binning information IMO gbintk.readthedocs.io/en/latest/

It seems we can assemble (reasonably simple populations) of co-existing strains with ONT data now. We assembled 6 single-contig Prevotella copri genomes of > 97% ANI for one metagenome. 4 of them were circular. (The largest metaFlye P. copri contig was 13.4% complete)

Announcing myloasm, a new long-read (ONT R10/PacBio) metagenome assembler that I've been working on during my postdoc in the Heng Li lab (@lh3lh3.bsky.social). myloasm-docs.github.io