Nanami Kubota (@nanamikubota.bsky.social)

Pseudomonas aeruginosa biofilm-deficient mutants undergo parallel evolution during chronic infection | Journal of Bacteriology We demonstrate that in a porcine full-thickness thermal injury wound model, a Pseudomonas aeruginosa mutant deficient in biofilm formation undergoes adaptive evolution by acquiring mutations that alter the outer membrane, either type IV pili (T4P) or lipopolysaccharide (LPS) mutations, that restores the deficient biofilm phenotype. We also observe a striking degree of mutational parallelism, at both the biosynthetic pathway and gene level, indicating the strong selective pressures experienced by these pathways during chronic wound infection. https://journals.asm.org/doi/10.1128/jb.00520-25