We’ve engineered stable cell systems to induce lipid scrambling of the plasma membrane with active forms of TMEM16F. Scrambling alone causes vesiculation (🔵TMEM16F-active; 🟠PS/Annexin V). Let us know if you want to try the cells out. Preprint: www.biorxiv.org/content/10.1...

Sorting nexin 10 regulates lysosomal ionic homeostasis via ClC-7 by controlling PI(3,5)P2, say Jing Ze Wu, Sergio Grinstein, Spencer Freeman and colleagues @sickkidsto.bsky.social: rupress.org/jcb/article/... #Immunology #Membrane #lipid #lysosomes #osteopetrosis

This work was done in tandem with cool work from the @spencerfreeman.bsky.social lab identifying novel regulators of PI3,5P2 in lysosomes rupress.org/jcb/article-...

Pemberton, Burke, Balla et al. report the generation & characterization of an engineered active fragment of PIKfyve and its use to generate extra PI(3,5)P2 in endomembrane compartments & test the function of PI(3,5)P2 #biosensors rupress.org/jcb/article/... #lipids #CellSignaling

New tools and regulators of lysosomal PI(3,5)P2! Lipidomics by Sin, Junko, Takehiko (Tokyo) and a new toolkit for PIKfyve/PI(3,5)P2 by Josh and Tamas (NIH). Check out JIm's paper here: hubs.la/Q03dym0b0 or: shorturl.at/yq9K8 and the paper by Balla, Burke, Pemberton et al. here: hubs.la/Q03dyfL60

Excited to post for the first time to present our exciting new paper @Nature. The fruit of massive work by Tommaso Vicanolo, @alazozcan.bsky.social and Jackson Li, and our superb collaborators Laiguan and Stefan Uderhardt. True teamwork! Free link: rdcu.be/eeeA2 www.nature.com/articles/s41...

Simultaneous CRISPR screening and spatial transcriptomics reveal intracellular, intercellular, and functional transcriptional circuits: Cell www.cell.com/cell/fulltex...

We've been studying mechanisms of resilience that protect lysosomes from damage and wrote a review on the topic. We consider lysosomal stress and damage on a spectrum. Please help me share as this is my first post 😀 and I don't have many followers! 🙏 Open Access: www.embopress.org/doi/full/10....