Karl Romanowicz
@kroman.bsky.social
π€ 266
π₯ 801
π 3
Principal Scientist
@synplexity.bsky.social
Synthetic Biology and Bioinformatics
pinned post!
Our new Science Advances paper is out! π The Plesa Lab's first, it builds on DropSynth technology as a proof of concept for large-scale synthetic gene libraries, showcasing a synthetic metagenomics approach to studying antibiotic resistance at scale. π
www.science.org/doi/10.1126/...
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Exploring antibiotic resistance in diverse homologs of the dihydrofolate reductase protein family through broad mutational scanning
DropSynth technology enables scalable and cost-effective exploration of antibiotic resistance across the DHFR protein family.
https://www.science.org/doi/10.1126/sciadv.adw9178
7 months ago
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reposted by
Karl Romanowicz
Calin Plesa
4 days ago
New lab preprint: if ML struggles with extrapolation, let's expand the diversity of training data with gene synth, DNA shuffling, and ML gen.... also lots of
#FluorescentProteins
#ProteinEngineering
#MachineLearning
#SyntheticBiology
1/n
add a skeleton here at some point
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π₯ My Variant Effects Seminar Series (VESS) talk is now on YouTube! I discuss how DropSynth-based gene synthesis reveals how bacterial DHFR variants evolve antibiotic resistance. Thanks to
@varianteffect.bsky.social
for the invite! π
www.youtube.com/watch?v=KiA9...
#VESS
#VariantEffects
#DropSynth
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"Large-scale synthetic gene libraries for decoding variant effects in antibiotic resistance"
YouTube video by Variant Effects
https://www.youtube.com/watch?v=KiA93lw5SPM
5 months ago
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reposted by
Karl Romanowicz
Atlas of Variant Effects Alliance
6 months ago
Next up! Two applications of MAVE! 𧬠DNA damage repair & cancer: Shelby Hemker (
@jacobkitzman.bsky.social
lab UMich) measures DNA repair of MUTYH variants. π¦ Antibiotic resistance:
@kroman.bsky.social
(βͺ@synplexity.bsky.social Univ. of Oregon) decodes how mutations drive resistance in DHFR.
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Our new Science Advances paper is out! π The Plesa Lab's first, it builds on DropSynth technology as a proof of concept for large-scale synthetic gene libraries, showcasing a synthetic metagenomics approach to studying antibiotic resistance at scale. π
www.science.org/doi/10.1126/...
loading . . .
Exploring antibiotic resistance in diverse homologs of the dihydrofolate reductase protein family through broad mutational scanning
DropSynth technology enables scalable and cost-effective exploration of antibiotic resistance across the DHFR protein family.
https://www.science.org/doi/10.1126/sciadv.adw9178
7 months ago
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12
7
reposted by
Karl Romanowicz
SynPlexity
12 months ago
We unlock breakthrough insights by delivering the best training sets in biologyβat unprecedented scale.
youtu.be/KuCaJTPHM2o
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Save 90% on DNA variant libraries today
YouTube video by IndieBio
https://youtu.be/KuCaJTPHM2o
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Permafrost thaw takes center stage on Ice & Fire Climate Change podcast! ποΈβοΈπ₯ S2 Ep2 "Layer" & Ep3 "Microbes Reawaken" explore carbon release & climate impacts. Hear my research! Tune in:
iceandfireclimatepodcast.weebly.com/s2-permafros...
#ClimateChange
#PermafrostResearch
#SciComm
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s2: permafrost thaw
podcast is available for listening on Apple Podcasts, Spotify, Overcast, and through the Public Radio Exchange (PRX) β β or listen below. Press "More Info" in each episode player to reveal show...
https://iceandfireclimatepodcast.weebly.com/s2-permafrost-thaw.html
about 1 year ago
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reposted by
Karl Romanowicz
Calin Plesa
about 1 year ago
New preprint! We carried out the largest complementation and inhibition study for diverse homologs in a single protein family (DHFR) to date. Highlights thread follows... 1/n π§΅
add a skeleton here at some point
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