Two-Year Field Trial of Genetically Engineered American Chestnut Reveals Greater Fungal Blight Tolerance Compared to Wild-Type Full-Sibling Trees https://www.biorxiv.org/content/10.1101/2025.11.07.687213v1

Genomic Perception Fusion: A Lightweight, Interpretable Kernel for Protein Functional Tuning https://www.biorxiv.org/content/10.1101/2025.11.06.686961v1

Rewiring protein function through genetically encoded oxidative chemistry https://www.biorxiv.org/content/10.1101/2025.11.06.686050v1

Cargo-directed assembly of nonviral nucleocapsid with controlled size https://www.biorxiv.org/content/10.1101/2025.11.06.684264v1

Modeling and minimization of dCas9-induced competition in CRISPRi-based genetic circuits https://www.biorxiv.org/content/10.1101/2025.11.05.686856v1

Genetic incorporation of diverse non-canonical amino acids for histidine substitution https://www.biorxiv.org/content/10.1101/2025.11.04.686677v1

Engineering of Pseudomonas putida KT2440 for Broad-Chain-Length 3-Hydroxy Fatty Acid Biosynthesis https://www.biorxiv.org/content/10.1101/2025.11.04.686531v1

Rhodo-Box: a Synthetic Biology Toolbox to Facilitate Metabolic Engineering of Rhodobacter sphaeroides https://www.biorxiv.org/content/10.1101/2025.10.31.685836v1

PHYCUT: Scalable multiplex CRISPR/Cas9 editing forgenome engineering in the diatom Phaeodactylumtricornutum https://www.biorxiv.org/content/10.1101/2025.10.31.685893v1

Engineered orthogonal translation systems from metagenomic libraries expand the genetic code https://www.biorxiv.org/content/10.1101/2025.10.30.685624v1

A comprehensive genetic toolkit for Zymomonas mobilis; allowing rapid, high-resolution, combinatorial engineering of metabolic pathways. https://www.biorxiv.org/content/10.1101/2025.10.29.685439v1

Optimized quantitative bacterial two-hybrid (qB2H) for protein-protein interaction assessment https://www.biorxiv.org/content/10.1101/2025.10.29.680610v1

Adaptive evolution of engineered Saccharomyces cerevisiae in favored and unusual chemical environments https://www.biorxiv.org/content/10.1101/2025.10.29.685190v1

In-Cell Synthesis of Nε-acetyl-L-lysine for Facile Protein Acetylation https://www.biorxiv.org/content/10.1101/2025.10.29.685286v1

Orthogonal Inducible Transcriptional Control Systems forInvestigation of Gene-Gene Interactions https://www.biorxiv.org/content/10.1101/2025.10.28.685021v1

CREation of an expanded plant memory gene circuit toolkit https://www.biorxiv.org/content/10.1101/2025.10.28.684748v1

Synthetic multicolor antigen-stabilizable nanobody platform for intersectional labelling and functional imaging. https://www.biorxiv.org/content/10.1101/2025.10.27.684934v1

TRANCERs: Engineering enhancers into autonomous tissue-specific expression cassettes https://www.biorxiv.org/content/10.1101/2025.10.27.684763v1

Allosteric Ligand-Aptamer Complexes Orchestrate Supramolecular or Transient Catalytic, Transcription and Fibrinogenesis Processes https://www.biorxiv.org/content/10.1101/2025.10.26.684577v1

PIONEER: A Periplasmic Display Platform for Synthetic Biology-Based Screening of Genetically Encoded Protein Regulators https://www.biorxiv.org/content/10.1101/2025.10.26.684622v1

MillionFull enables massive, full-length enzyme sequence-fitness data collection at low cost for machine learning-guided enzyme engineering https://www.biorxiv.org/content/10.1101/2025.10.24.684421v1

A high throughput flow cytometry assay for quantifying type 3 secretion system assembly in Salmonella https://www.biorxiv.org/content/10.1101/2025.10.25.684470v1

QuantGUV: Quantifying Encapsulation Efficiency of Small Molecules in GUVs https://www.biorxiv.org/content/10.1101/2025.10.23.684161v1

Combinatorial metabolic engineering of alkane biosynthesis in the osmotolerant yeast Debaryomyces hansenii CBS 767 https://www.biorxiv.org/content/10.1101/2025.10.23.684212v1

Unraveling the Transcriptional Landscape within a Minimized Bacterium via Comparative Analysis https://www.biorxiv.org/content/10.1101/2025.10.23.681674v1

Machine learning guided engineering of conditional split inteins for regulated protein splicing in mammalian cells https://www.biorxiv.org/content/10.1101/2025.10.21.683710v1

Vesicle-templated self-assembly of freestanding multi-μm DNA shells https://www.biorxiv.org/content/10.1101/2025.10.21.683722v1

Gene library deep sequencing for protein super-family profiling https://www.biorxiv.org/content/10.1101/2025.10.20.682913v1

Direct Genome Transfer from Acholeplasma laidlawii to Yeast https://www.biorxiv.org/content/10.1101/2025.10.17.683135v1

Engineering human protein switches for functional control of CARs and transcription factors via oral drug administration https://www.biorxiv.org/content/10.1101/2025.10.17.683068v1

Resolving replication incompatibility between chloroplast and conjugative plasmids in E. coli https://www.biorxiv.org/content/10.1101/2025.10.16.682956v1

Anti-CD206 CAR T Cell Treatment Restores Fibrosis-Induced Loss of Dermal White Adipose Tissue https://www.biorxiv.org/content/10.1101/2025.10.16.682860v1

Pharmacophore-driven antibody discovery on the yeast surface https://www.biorxiv.org/content/10.1101/2025.10.16.682880v1

Assembly and cell-free expression of a partial genome for the synthetic cell https://www.biorxiv.org/content/10.1101/2025.10.16.682769v1

Odyssey: reconstructing evolution through emergent consensus in the global proteome https://www.biorxiv.org/content/10.1101/2025.10.15.682677v1

Mapping the diverse topologies of protein-protein interaction fitness landscapes https://www.biorxiv.org/content/10.1101/2025.10.14.682342v1

Massively parallel assay of human splice variants reveals cis-regulatory drivers of disease-associated and cell type-specific splicing regulation https://www.biorxiv.org/content/10.1101/2025.10.12.681955v1

High-Throughput De Novo Protein Design Yields Novel Immunomodulatory Agonists https://www.biorxiv.org/content/10.1101/2025.10.12.681920v1

Geometric Tuning of Cytokine Receptor Association Modulates Synthetic Agonist Signaling https://www.biorxiv.org/content/10.1101/2025.10.12.681819v1

Multiplex design and discovery of proximity handles for programmable proteome editing https://www.biorxiv.org/content/10.1101/2025.10.13.681693v1

Sourdough starter-inspired living materials grown from probiotic consortia with engineered division-of-labor https://www.biorxiv.org/content/10.1101/2025.10.09.681537v1

iFLinkC-EZ: A scalable and automatable method for the assembly of complex fusion proteins and multi-gene expression constructs based on the iFLinkC framework https://www.biorxiv.org/content/10.1101/2025.10.08.680606v1

Sequence-free landscape inference for directed evolution https://www.biorxiv.org/content/10.1101/2025.10.08.681123v1

Genome-wide screening reveals producer-cell modifications that improve virus-like particle production and delivery potency https://www.biorxiv.org/content/10.1101/2025.10.07.681010v1

Pseudomonas putida JM37 as a novel bacterial chassis for ethylene glycol upcycling https://www.biorxiv.org/content/10.1101/2025.10.07.680640v1

SHARK: a specialized host for assembling R6K plasmids https://www.biorxiv.org/content/10.1101/2025.10.06.680659v1

Mapping the evolution of computationally designed protein binders https://www.biorxiv.org/content/10.1101/2025.10.04.680454v1

A Modular Genetic Code Expansion Approach to Site-Specific Lysine Acylations https://www.biorxiv.org/content/10.1101/2025.10.03.680225v1

Rapid assembly of functional modules for generating human artificial chromosome constructs compatible with epigenetic centromere seeding https://www.biorxiv.org/content/10.1101/2025.10.02.680129v1

Split inteins for generating combinatorial non-ribosomal peptide libraries https://www.biorxiv.org/content/10.1101/2025.10.02.680031v1