Decoupling Central Metabolism from Catabolite Repression Enables Robust Cellulosic Sugar Co-consumption in E. coli https://www.biorxiv.org/content/10.64898/2025.12.27.696699v1

Increasing the effective gene drive homing rate by targeting the haploinsufficient spermatogenesis gene KLHL10 https://www.biorxiv.org/content/10.64898/2025.12.22.695236v1

A Hybridization-Based Novel DNA Storage Technology with Rapid Read Performance https://www.biorxiv.org/content/10.64898/2025.12.24.696143v1

Engineered translation release factor 1 suppresses disease-causing nonsense mutations and enables multi-site nonstandard amino acids incorporation https://www.biorxiv.org/content/10.64898/2025.12.24.695955v1

Automated, modular assembly of reconstituted cell-free systems from in vitro-produced components https://www.biorxiv.org/content/10.64898/2025.12.23.696326v1

Enzyme-driven phase separation of synthetic condensates enables self-organizing compartments and protective microenvironments https://www.biorxiv.org/content/10.64898/2025.12.24.696465v1

Engineering Orthogonal Carbon Dissimilation: A Gluconate Bypass Platform for Robust Stationary-Phase Biomanufacturing https://www.biorxiv.org/content/10.64898/2025.12.23.696260v1

Predictive design of tissue-specific mammalian enhancers that function in vivo in the mouse embryo https://www.biorxiv.org/content/10.64898/2025.12.22.695948v1

A scalable transposon mutagenesis system for non-model bacteria https://www.biorxiv.org/content/10.64898/2025.12.22.696024v1

Coiled-coil scaffolds for Targeted Protein Degradation https://www.biorxiv.org/content/10.64898/2025.12.22.695764v1

Demonstrating and engineering the de novo synthesis of two aromatic chemicals estragole and anethole in Escherichia coli https://www.biorxiv.org/content/10.64898/2025.12.22.695922v1

Defining bottlenecks and physiological impact of an orthogonal translation initiation system https://www.biorxiv.org/content/10.64898/2025.12.21.695813v1

Multiplexed Pan Soluble Ligandome Assaying via OASIS https://www.biorxiv.org/content/10.64898/2025.12.21.695830v1

De novo designed bifunctional proteins for targeted protein degradation https://www.biorxiv.org/content/10.64898/2025.12.22.695915v1

Synthetic Yarrowia lipolytica consortium for efficient conversion of lignocellulosic oligosaccharides into lipids https://www.biorxiv.org/content/10.64898/2025.12.19.695487v1

Regulatable In Vivo Gene Expression via Adaptamers https://www.biorxiv.org/content/10.64898/2025.12.21.695777v1

An engineered multi-step differentiation program in Escherichia coli for self-organized spatial patterning https://www.biorxiv.org/content/10.64898/2025.12.19.695475v1

Biomolecular condensation using de novo designed globular proteins https://www.biorxiv.org/content/10.64898/2025.12.19.695468v1

Engineering chimeric DNA polymerases for DNA movable type storage https://www.biorxiv.org/content/10.64898/2025.12.18.694503v1

Transfer of the synechan biosynthesis and regulatory pathway enables sulfated polysaccharide production in Synechococcus elongatus PCC 7942 https://www.biorxiv.org/content/10.64898/2025.12.18.694786v1

Growth control as a central regulator for tuning the cellular context https://www.biorxiv.org/content/10.64898/2025.12.19.695408v1

PURE makes PURE: reconstitution of the PURE cell-free system from self-synthesized non-ribosomal proteins https://www.biorxiv.org/content/10.64898/2025.12.17.694911v1

Horseradish peroxidase as an electrochemical reporter protein for cell-free biosensors https://www.biorxiv.org/content/10.64898/2025.12.17.694927v1

Tunable light-focusing behavior of engineered bacterial microlenses with controllable shapes https://www.biorxiv.org/content/10.64898/2025.12.17.694918v1

A scalable architecture for tuning multistate differentiation ratios in synthetic microbial consortia https://www.biorxiv.org/content/10.64898/2025.12.17.694810v1

Computer-assisted dissection of the DNA mismatch recognition mechanism of the MutS protein https://www.biorxiv.org/content/10.64898/2025.12.16.694542v1

De novo design of protein competitors for small molecule immunosensing https://www.biorxiv.org/content/10.64898/2025.12.16.694474v1

Quantitative profiling of millions of nucleotides reveals sequence-encoded interactions that govern plasmid propagation https://www.biorxiv.org/content/10.64898/2025.12.15.694402v1

Rigorous Quantitative Analysis of Nonlinear Uncertain Biomolecular Systems using Validated Methods https://www.biorxiv.org/content/10.64898/2025.12.13.693835v1

Engineered AAV Capsids with Enhanced Extracellular Vesicle Loading via Rational Design and Directed Evolution https://www.biorxiv.org/content/10.64898/2025.12.12.693981v1

Mechanisms of soil carbon preservation illuminated by model mineral-associated organic matter https://www.biorxiv.org/content/10.64898/2025.12.12.694042v1

Mapping the phenotypic landscape of a transcriptional repressor using Deep Mutational Scanning and Growth-based Quantitative Sequencing https://www.biorxiv.org/content/10.64898/2025.12.11.693801v1

Multiobjective design of growth media with genome-scale metabolic models and Bayesian optimization https://www.biorxiv.org/content/10.64898/2025.12.12.693982v1

Anti-CRISPR-mediated continuous directed evolution of CRISPR-Cas9 in human cells https://www.biorxiv.org/content/10.64898/2025.12.11.693673v1

Innovative Prevention Approach for Pan Allergens-Induced Allergy: Effective Regulation of Heat Shock Protein 70 and Profilin 3 by siRNA-Loaded Nanomaterial https://www.biorxiv.org/content/10.64898/2025.12.09.693215v1

Going beyond size: Exploring the metabolic burden inPseudomonas putida during heterologous proteinproduction https://www.biorxiv.org/content/10.64898/2025.12.09.693165v1

Structure-based design of antibody repertoires with drug-like properties https://www.biorxiv.org/content/10.64898/2025.12.10.693474v1

A transcription factor-responsive enhancer discovery platform for targeted immunotherapy https://www.biorxiv.org/content/10.64898/2025.12.08.693012v1

Engineering a Covalent Linkage into a Dimeric De Novo Enzyme Reveals a Novel Life-Sustaining Mechanism https://www.biorxiv.org/content/10.64898/2025.12.09.693225v1

Structure and evolution-guided design of minimal RNA-guided nucleases https://www.biorxiv.org/content/10.64898/2025.12.08.692503v1

Conjugation Based CRISPR Antifungals (COBRA) https://www.biorxiv.org/content/10.64898/2025.12.06.692781v1

Elucidation and engineering of arabinofuranosyltransferase to enable total de novo biosynthesis of paris saponins in yeast https://www.biorxiv.org/content/10.64898/2025.12.07.692791v1

Generative design and construction of functional plasmids with a DNA language model https://www.biorxiv.org/content/10.64898/2025.12.06.692736v1

A genetic platform for a biocementation bacterium https://www.biorxiv.org/content/10.64898/2025.12.05.692644v1

Design of post-translational, ligand-controlled inverters and switches https://www.biorxiv.org/content/10.64898/2025.12.05.692672v1

Repurposing the diatom periplastidial compartment for heterologous terpenoid production https://www.biorxiv.org/content/10.64898/2025.12.05.692597v1

Biochemical Upcycling of PET via Glycolysis and Engineered Microbial Consortia https://www.biorxiv.org/content/10.64898/2025.12.04.692332v1

Stimuli-Expressed Protein Qubits for Quantum Quantification of Heavy Metals in Natural Water https://www.biorxiv.org/content/10.64898/2025.12.04.692285v1

Synthetic Modular Cyanobacterial Consortium Enables Carbon-Negative Production of Glycerol and Derivatives https://www.biorxiv.org/content/10.64898/2025.12.04.692262v1

Design and synthesis of mirror image fluorescent nucleotide reversible terminator, (L)-3'-O-azidomethyl-dGTP-N3-fluorophore, and (D)-9°N mutant DNA polymerase for mirror image (L)-DNA sequencing by synthesis https://www.biorxiv.org/content/10.64898/2025.12.03.692211v1