BLAST: A blue light-assisted secretion toolkit tunable by reversible protein-protein interactions https://www.biorxiv.org/content/10.64898/2026.03.30.715452v1

A CURE for synthetic regulation of gene expression: Rapid screening of guide RNA efficacy as a framework for enabling undergraduate research in plant synthetic biology https://www.biorxiv.org/content/10.64898/2026.03.31.715601v1

Turning of (Ph)age: Expanding the therapeutic potential of our viral allies https://www.biorxiv.org/content/10.64898/2026.03.30.715334v1

Cytotoxicity-based High-throughput Screening System for CAR T Cell https://www.biorxiv.org/content/10.64898/2026.03.31.715247v1

Lipid-conjugated DNA enables on-demand delivery of lipids and proteins to synthetic cells https://www.biorxiv.org/content/10.64898/2026.03.30.715215v1

Tardigrade-Derived Strategy for Low-Cost Storage of Cell-Free Expression Lysates https://www.biorxiv.org/content/10.64898/2026.03.29.715078v1

An expedient, biology-laboratory-compatible method for preparing functional perfluoropolyether fluorosurfactants for droplet microfluidics https://www.biorxiv.org/content/10.64898/2026.03.28.714914v1

AlphaFold Database expands to proteome-scale quaternary structures https://www.biorxiv.org/content/10.64898/2026.03.27.714458v1

Engineering bacterial combinatorial promoters for two-input chemical AND switching https://www.biorxiv.org/content/10.64898/2026.03.25.714203v1

Endogenous intronic RNA tightly controls Cas9/CRISPR-mediated gene editing in human cells https://www.biorxiv.org/content/10.64898/2026.03.24.714022v1

CombinGym: a benchmark platform for machine learning-assisted design of combinatorial protein variants https://www.biorxiv.org/content/10.64898/2026.03.24.714074v1

Modulating Innate Immune Responses to Curli Fibers Through Protein Engineering https://www.biorxiv.org/content/10.64898/2026.03.23.713613v1

Transient contractility attenuation reprograms epithelial cells into a protrusion-driven state that drives tissue fluidization https://www.biorxiv.org/content/10.64898/2026.03.23.713577v1

Improved Biosynthesis of Ethylene Glycol from Xylose in Engineered E. coli Utilizing Two-Stage Dynamic Control https://www.biorxiv.org/content/10.64898/2026.03.24.713905v1

Optimization of PURE system composition using automation and active learning https://www.biorxiv.org/content/10.64898/2026.03.23.713685v1

Substrate transport limits phenylalanine ammonia-lyase activity in engineered Lacticaseibacillus rhamnosus GG https://www.biorxiv.org/content/10.64898/2026.03.19.713057v1

Multi-lab, Multi-enzyme Study Demonstrates the Versatility of Bacterial Microcompartment Shells as a Modular Platform for Confined Biocatalysis https://www.biorxiv.org/content/10.64898/2026.03.18.712704v1

A genetically encoded local learning rule enables physical learning in engineered bacteria https://www.biorxiv.org/content/10.64898/2026.03.18.712691v1

Seven inducible promoters for Zymomonas mobilis https://www.biorxiv.org/content/10.64898/2026.03.17.712268v1

A Toolbox for Biomanufacturing of Functionalised PHA Nanoparticles with C. necator https://www.biorxiv.org/content/10.64898/2026.03.17.712365v1

Context-dependent determinants of CRISPR-Cas9 editing efficiency revealed through cross-species endogenous editing analysis https://www.biorxiv.org/content/10.64898/2026.03.18.712093v1

Behavioral, Physiological, and Transcriptional Mechanisms of Memory in a Synthetic Living Construct https://www.biorxiv.org/content/10.64898/2026.03.17.712168v1

Robust Multiplicative Control in Chemical Reaction Networks -Extended Version https://www.biorxiv.org/content/10.64898/2026.03.15.711890v1

Programmable bacterial adhesion to plastic surfaces for enhanced biodegradation https://www.biorxiv.org/content/10.64898/2026.03.16.710745v1

Improved vector toolkit for genome writing in mammalian cells https://www.biorxiv.org/content/10.64898/2026.03.15.711894v1

Selection-free whole genome transplantation revives dead microbes https://www.biorxiv.org/content/10.64898/2026.03.13.711674v1

Continuous hypermutation and evolution of noncanonical amino acid synthases https://www.biorxiv.org/content/10.64898/2026.03.11.711232v1

Built-in integrated living electronics: from biosynthesis tomodulation of neuronal function https://www.biorxiv.org/content/10.64898/2026.03.12.710354v1

Optimization of the glucosinolate core pathway for production of simple glucosinolates in Escherichia coli https://www.biorxiv.org/content/10.64898/2026.03.12.711178v1

Hydration-dehydration cycles drive compartment dynamics in minimal protocells https://www.biorxiv.org/content/10.64898/2026.03.10.710739v1

Reaction-Conditioned Enzyme Discovery with Multimodal Deep Learning https://www.biorxiv.org/content/10.64898/2026.03.09.710689v1

Fung-AI: An AI/ML-driven pipeline for antifungal peptide discovery https://www.biorxiv.org/content/10.64898/2026.03.09.710548v1

Impact of retroactivity on information flows in engineered synthetic biological circuits https://www.biorxiv.org/content/10.64898/2026.03.05.709940v1

Synthetic Genome Shuffling of Poxviruses through Yeast for Next-Generation Oncolytic Platforms https://www.biorxiv.org/content/10.64898/2026.03.06.710085v1

Automated mini-bioreactors reveal the temporal dynamics and multi-omics responses of CRISPRi knockdowns in Pseudomonas putida https://www.biorxiv.org/content/10.64898/2026.03.06.709552v1

Engineering S. cerevisiae extracellular vesicles using synthetic biology https://www.biorxiv.org/content/10.64898/2026.03.06.710173v1

Genetically Programmed Shape-morphing of Engineered Living Materials https://www.biorxiv.org/content/10.64898/2026.03.05.709855v1

The Limits of Sequence-Based Biosecurity Screening Tools in the Age of AI-Assisted Protein Design https://www.biorxiv.org/content/10.64898/2026.03.04.709671v1

Electrostatic Engineering of Phosphoketolase Enhances Activity on Small Non-phosphorylated Sugars and Improves Cell-Free ATP Regeneration from Inexpensive C2-Substrates https://www.biorxiv.org/content/10.64898/2026.03.04.709640v1

Generative AI designs functional thiolation domains for reprogramming non-ribosomal peptide synthetases https://www.biorxiv.org/content/10.64898/2026.03.03.709401v1

High Diversity Gene Libraries Facilitate Machine Learning Guided Exploration of Fluorescent Protein Sequence Space https://www.biorxiv.org/content/10.64898/2026.03.01.706892v1

Evaluating AI-Assisted Customer Verification for Synthetic Nucleic Acid Screening https://www.biorxiv.org/content/10.64898/2026.02.27.708645v1

CADGE 2.0, Transcription-Translation-Coupled DNA Replication is Improved in a Chemically Modified Cell-Free System https://www.biorxiv.org/content/10.64898/2026.02.27.708527v1

Combinatorial optimization of protein systems in synthetic cells https://www.biorxiv.org/content/10.64898/2026.02.25.707944v1

CyanOperon: an operon building expansion for the CyanoGate MoClo toolkit https://www.biorxiv.org/content/10.64898/2026.02.24.707249v1

A portable orthogonal replication system enables continuous gene evolution near the biological speed limit https://www.biorxiv.org/content/10.64898/2026.02.20.706958v1

Efficient cell-free evolution of RNA polymerases by droplet microfluidics https://www.biorxiv.org/content/10.64898/2026.02.23.707346v1

Nucleotide-level chemical reaction network modeling enables quantitative prediction of reconstituted cell-free expression system https://www.biorxiv.org/content/10.64898/2026.02.22.707325v1

Heterologous Rieske non-heme iron monooxygenases enable efficient microbial conversion of lignin guaiacol to adipic acid https://www.biorxiv.org/content/10.64898/2026.02.23.707458v1

Programmable nanobody circuits for cell selection https://www.biorxiv.org/content/10.64898/2026.02.22.707289v1