bioRxiv Synthetic Biology
@biorxiv-synthbio.bsky.social
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Two-Year Field Trial of Genetically Engineered American Chestnut Reveals Greater Fungal Blight Tolerance Compared to Wild-Type Full-Sibling Trees
https://www.biorxiv.org/content/10.1101/2025.11.07.687213v1
about 19 hours ago
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Genomic Perception Fusion: A Lightweight, Interpretable Kernel for Protein Functional Tuning
https://www.biorxiv.org/content/10.1101/2025.11.06.686961v1
2 days ago
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Rewiring protein function through genetically encoded oxidative chemistry
https://www.biorxiv.org/content/10.1101/2025.11.06.686050v1
3 days ago
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1
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Cargo-directed assembly of nonviral nucleocapsid with controlled size
https://www.biorxiv.org/content/10.1101/2025.11.06.684264v1
3 days ago
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Modeling and minimization of dCas9-induced competition in CRISPRi-based genetic circuits
https://www.biorxiv.org/content/10.1101/2025.11.05.686856v1
4 days ago
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Genetic incorporation of diverse non-canonical amino acids for histidine substitution
https://www.biorxiv.org/content/10.1101/2025.11.04.686677v1
4 days ago
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Engineering of Pseudomonas putida KT2440 for Broad-Chain-Length 3-Hydroxy Fatty Acid Biosynthesis
https://www.biorxiv.org/content/10.1101/2025.11.04.686531v1
5 days ago
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Rhodo-Box: a Synthetic Biology Toolbox to Facilitate Metabolic Engineering of Rhodobacter sphaeroides
https://www.biorxiv.org/content/10.1101/2025.10.31.685836v1
8 days ago
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PHYCUT: Scalable multiplex CRISPR/Cas9 editing forgenome engineering in the diatom Phaeodactylumtricornutum
https://www.biorxiv.org/content/10.1101/2025.10.31.685893v1
9 days ago
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Engineered orthogonal translation systems from metagenomic libraries expand the genetic code
https://www.biorxiv.org/content/10.1101/2025.10.30.685624v1
10 days ago
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A comprehensive genetic toolkit for Zymomonas mobilis; allowing rapid, high-resolution, combinatorial engineering of metabolic pathways.
https://www.biorxiv.org/content/10.1101/2025.10.29.685439v1
10 days ago
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Optimized quantitative bacterial two-hybrid (qB2H) for protein-protein interaction assessment
https://www.biorxiv.org/content/10.1101/2025.10.29.680610v1
11 days ago
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Adaptive evolution of engineered Saccharomyces cerevisiae in favored and unusual chemical environments
https://www.biorxiv.org/content/10.1101/2025.10.29.685190v1
11 days ago
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In-Cell Synthesis of Nε-acetyl-L-lysine for Facile Protein Acetylation
https://www.biorxiv.org/content/10.1101/2025.10.29.685286v1
11 days ago
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Orthogonal Inducible Transcriptional Control Systems forInvestigation of Gene-Gene Interactions
https://www.biorxiv.org/content/10.1101/2025.10.28.685021v1
12 days ago
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CREation of an expanded plant memory gene circuit toolkit
https://www.biorxiv.org/content/10.1101/2025.10.28.684748v1
12 days ago
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Synthetic multicolor antigen-stabilizable nanobody platform for intersectional labelling and functional imaging.
https://www.biorxiv.org/content/10.1101/2025.10.27.684934v1
13 days ago
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TRANCERs: Engineering enhancers into autonomous tissue-specific expression cassettes
https://www.biorxiv.org/content/10.1101/2025.10.27.684763v1
13 days ago
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Allosteric Ligand-Aptamer Complexes Orchestrate Supramolecular or Transient Catalytic, Transcription and Fibrinogenesis Processes
https://www.biorxiv.org/content/10.1101/2025.10.26.684577v1
14 days ago
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PIONEER: A Periplasmic Display Platform for Synthetic Biology-Based Screening of Genetically Encoded Protein Regulators
https://www.biorxiv.org/content/10.1101/2025.10.26.684622v1
14 days ago
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MillionFull enables massive, full-length enzyme sequence-fitness data collection at low cost for machine learning-guided enzyme engineering
https://www.biorxiv.org/content/10.1101/2025.10.24.684421v1
15 days ago
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A high throughput flow cytometry assay for quantifying type 3 secretion system assembly in Salmonella
https://www.biorxiv.org/content/10.1101/2025.10.25.684470v1
15 days ago
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QuantGUV: Quantifying Encapsulation Efficiency of Small Molecules in GUVs
https://www.biorxiv.org/content/10.1101/2025.10.23.684161v1
17 days ago
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Combinatorial metabolic engineering of alkane biosynthesis in the osmotolerant yeast Debaryomyces hansenii CBS 767
https://www.biorxiv.org/content/10.1101/2025.10.23.684212v1
17 days ago
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Unraveling the Transcriptional Landscape within a Minimized Bacterium via Comparative Analysis
https://www.biorxiv.org/content/10.1101/2025.10.23.681674v1
17 days ago
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Machine learning guided engineering of conditional split inteins for regulated protein splicing in mammalian cells
https://www.biorxiv.org/content/10.1101/2025.10.21.683710v1
19 days ago
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Vesicle-templated self-assembly of freestanding multi-μm DNA shells
https://www.biorxiv.org/content/10.1101/2025.10.21.683722v1
19 days ago
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Gene library deep sequencing for protein super-family profiling
https://www.biorxiv.org/content/10.1101/2025.10.20.682913v1
19 days ago
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Direct Genome Transfer from Acholeplasma laidlawii to Yeast
https://www.biorxiv.org/content/10.1101/2025.10.17.683135v1
23 days ago
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Engineering human protein switches for functional control of CARs and transcription factors via oral drug administration
https://www.biorxiv.org/content/10.1101/2025.10.17.683068v1
23 days ago
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Resolving replication incompatibility between chloroplast and conjugative plasmids in E. coli
https://www.biorxiv.org/content/10.1101/2025.10.16.682956v1
23 days ago
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Anti-CD206 CAR T Cell Treatment Restores Fibrosis-Induced Loss of Dermal White Adipose Tissue
https://www.biorxiv.org/content/10.1101/2025.10.16.682860v1
24 days ago
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Pharmacophore-driven antibody discovery on the yeast surface
https://www.biorxiv.org/content/10.1101/2025.10.16.682880v1
24 days ago
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Assembly and cell-free expression of a partial genome for the synthetic cell
https://www.biorxiv.org/content/10.1101/2025.10.16.682769v1
24 days ago
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6
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Odyssey: reconstructing evolution through emergent consensus in the global proteome
https://www.biorxiv.org/content/10.1101/2025.10.15.682677v1
25 days ago
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Mapping the diverse topologies of protein-protein interaction fitness landscapes
https://www.biorxiv.org/content/10.1101/2025.10.14.682342v1
26 days ago
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Massively parallel assay of human splice variants reveals cis-regulatory drivers of disease-associated and cell type-specific splicing regulation
https://www.biorxiv.org/content/10.1101/2025.10.12.681955v1
27 days ago
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High-Throughput De Novo Protein Design Yields Novel Immunomodulatory Agonists
https://www.biorxiv.org/content/10.1101/2025.10.12.681920v1
27 days ago
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Geometric Tuning of Cytokine Receptor Association Modulates Synthetic Agonist Signaling
https://www.biorxiv.org/content/10.1101/2025.10.12.681819v1
27 days ago
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Multiplex design and discovery of proximity handles for programmable proteome editing
https://www.biorxiv.org/content/10.1101/2025.10.13.681693v1
27 days ago
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Sourdough starter-inspired living materials grown from probiotic consortia with engineered division-of-labor
https://www.biorxiv.org/content/10.1101/2025.10.09.681537v1
30 days ago
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iFLinkC-EZ: A scalable and automatable method for the assembly of complex fusion proteins and multi-gene expression constructs based on the iFLinkC framework
https://www.biorxiv.org/content/10.1101/2025.10.08.680606v1
about 1 month ago
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Sequence-free landscape inference for directed evolution
https://www.biorxiv.org/content/10.1101/2025.10.08.681123v1
about 1 month ago
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Genome-wide screening reveals producer-cell modifications that improve virus-like particle production and delivery potency
https://www.biorxiv.org/content/10.1101/2025.10.07.681010v1
about 1 month ago
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Pseudomonas putida JM37 as a novel bacterial chassis for ethylene glycol upcycling
https://www.biorxiv.org/content/10.1101/2025.10.07.680640v1
about 1 month ago
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SHARK: a specialized host for assembling R6K plasmids
https://www.biorxiv.org/content/10.1101/2025.10.06.680659v1
about 1 month ago
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Mapping the evolution of computationally designed protein binders
https://www.biorxiv.org/content/10.1101/2025.10.04.680454v1
about 1 month ago
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A Modular Genetic Code Expansion Approach to Site-Specific Lysine Acylations
https://www.biorxiv.org/content/10.1101/2025.10.03.680225v1
about 1 month ago
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Rapid assembly of functional modules for generating human artificial chromosome constructs compatible with epigenetic centromere seeding
https://www.biorxiv.org/content/10.1101/2025.10.02.680129v1
about 1 month ago
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Split inteins for generating combinatorial non-ribosomal peptide libraries
https://www.biorxiv.org/content/10.1101/2025.10.02.680031v1
about 1 month ago
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