Had fun annotating glycopeptides from DIA-MS glycoproteomics by looking at similar MS spectra, glycan biosynthetic pathways. Awesome collaboration with Alejandro Gomez Toledo and Johan Malmström from Lund Univ and James Sorrentino in my lab. #glycotime doi.org/10.1080/1942...

Why do some cells produce more protein than others? Jasmine Tat (UCSD & Amgen) conducted a detailed study into the single-cell transcriptome, while measuring mAb production in single cells too with SEC-seq. Check out the preprint! doi.org/10.64898/202...

Our team of ~50 researchers built a major update on the CHO genome-scale metabolic model. This includes 3D enzyme structures, and it helped us understand better how cells suppress glucose catabolism when the Warburg metabolism is suppressed. authors.elsevier.com/a/1mxeh8YyDf...

Congrats to Dr. Jasmine Tat, who proved that a great PhD thesis is possible while conducting her research while working in industry. 1 great paper in Cell Systems, and more innovative ones coming soon! If only I could have convinced her to obey the sign behind her. doi.org/10.1016/j.ce...

Check out our latest work on inferring metabolite-protein-mediated cell-cell communication. Led by @erickarmingol.bsky.social doi.org/10.1093/bioi...

Download our large database of funding opportunities for early-career faculty and researchers Download it here: research.jhu.edu/rdt/funding-... 433 funding opportunities.

~1/3 of protein-coding genes are made in the secretory pathway. We curated >1000 of its machinery proteins, opening up new opportunities to engineer cells for drug production, to study cell-cell interactions, or modeling. @cp-cellsystems.bsky.social paper -> authors.elsevier.com/a/1mFMc8YyDf...

Congrats to Mina Wu for an excellent PhD defense on improving production of mAbs and vaccine proteins by studying protein-protein interactions in the production cells. See doi.org/10.1002/biot... and doi.org/10.1016/j.ym...

Got a great score for a vaccine proposal and yet fearful about if it will be funded. Maybe my next proposal will fare better? "Intersectional Bias, Woke Adjuvants, and Inclusive Nanoparticle Vaccines: Disinformation-Resilient Immunity in Trans-genic Mice and Women". Fundable?

Looking forward to this collaboration with Ginkgo www.genengnews.com/topics/biopr...

Monty Python understood p-hacking

😭

I love this visual of what I've been telling my kids / students, but I add that immediate answers rob our creativity too. I regularly ask myself "i can prompt this, but how important will my struggle be here?" Some tasks are perfect for AI. For others I choose to stimulate creativity & learning.

Why do some recomb proteins express better than others? After expressing >2000 proteins from the human secretome in CHO cells, we identified the protein features and host cell pathways linked to poor expression w/ @JohanRockberg lab. www.pnas.org/doi/abs/10.1...

Robert Francis Kennedy Junior

The team at BioF:GREAT is always on the lookout for new protein design models that care about more than just the backbone! We are excited to try the new RFdiffusion3 (RFD3)! 🚀 New preprint from David Baker!🚀 🧪 #ProteinDesign #DeepLearning #ComputationalBiology www.biorxiv.org/content/10.1...

Honored to have our recent work on mapping out the cell machinery regulating protein secretion highlighted at GEN www.genengnews.com/topics/biopr...

Excitng collaboration in which we expressed a hepatitis c vaccine candidate in a panel of glycoengineered CHO cell lines. This allowed us to find a specific glycan structure that greatly improved immunogenecity, even for more distant strains #glycotime www.nature.com/articles/s41...

While our first discovery of this occurred just weeks into the shutdown, it's great to see it off bioRxiv now. SARS-CoV-2 infectivity can be modulated through bacterial grooming of the glycocalyx. Great collab with Knight and Esko labs and many others #glycotime journals.asm.org/doi/10.1128/...

Took me a while to get the new uga address, but my lab website is finally live. lewislab.uga.edu

With all of the US government science chaos going on, I'm curious if anyone knows which governments are seeing the great opportunity to recruit top labs and tech companies, to bolster their international presence, and to reverse the brain drain.

🚨 Join us for the next GlyGen Webinar! 🗓 Feb 4, 2025 | 🕒 10 AM EST 🎙️ Speaker: Dr. Parastoo Azadi, CCRC, @universityofga.bsky.social 💡 Research, Collaboration, Service & Training at CCRC 📑 Details: wiki.glygen.org Also, don't miss our Jan webinar on Jan 28th! #glycotime

After ~8 years, happy to share work done w/ @natelewis3.bsky.social showing you can eliminate the Warburg effect in mammalian cells w/out impacting growth rate is out now in @natmetabolism.bsky.social! www.nature.com/articles/s42... #science #biotech #cancermetabolism Thread below:

I don't think that 101 years ago, Otto Warburg was aware how broadly relevant his discovery was. Check out our new paper out today in Nature Metabolism, wherein we engineered the Warburg effect out of mammalian cells to improve biologics production urldefense.com/v3/__https:/...

Calling all glyco-enthusiasts!! We have an opening for a tenure-track faculty member at any rank in the Complex Carbohydrate Research Center (ccrc.uga.edu) in the areas of plant or microbial glycoscience. Come join our amazing team. #glycotime www.ugajobsearch.com/postings/400...

These past few years have seen a rapid expansion of cell-cell communication research. Check out our new review of the diverse methods leveraging single-cell and spatial omics: rdcu.be/dwkND