Jörg Stülke
@joergstuelke.bsky.social
📤 219
📥 142
📝 67
Cool new paper on the structure and possible function of the type VII ABC transporter YtrEF in cell wall remodeling in Bacillus subtilis!
#SubtiWiki
www.sciencedirect.com/science/arti...
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Structural analysis of a Gram-positive type VII ABC transporter induced by cell wall-targeting antibiotics
Bacteria utilize a variety of mechanisms to remodel the cell wall in response to environmental and antimicrobial stress. In the model organism Bacillu…
https://www.sciencedirect.com/science/article/pii/S0969212625003910
14 days ago
0
9
2
Yesterday, we had the graduation ceremony and PhD party of
@c-elfmann.bsky.social
.
@uni-goettingen.de
@imprsgs.bsky.social
#SubtiWiki
18 days ago
0
3
0
Today
@c-elfmann.bsky.social
, the mastermind of
#SubtiWiki
successfully defended his PhD thesis! The 50. PhD graduate from the group! Congratulations, Christoph!
@uni-goettingen.de
@imprsgs.bsky.social
26 days ago
4
14
1
We have started to add references to the
#SubtiWiki
metabolite pages. Please leg us know if your paper/ metabolite is missing!
@c-elfmann.bsky.social
@imprsgs.bsky.social
@johnhelmann.bsky.social
27 days ago
0
6
1
Sneak preview of the next upcoming
#SubtiWiki
feature: All proteins are assigned to clusters sharing similar structures! Brought to you by Oskar Geissel and
@c-elfmann.bsky.social
.
@imprsgs.bsky.social
about 1 month ago
1
6
2
Identification of genetic interactions in Bacillus subtilis cell division. Congratulations, Byoung-Mo Koo, Carol Gross, and all involved!
@cp-cellsystems.bsky.social
#subtiwiki
www.cell.com/cell-systems...
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Comprehensive genetic interaction analysis of the Bacillus subtilis envelope using double-CRISPRi
Koo et al. apply genome-scale double-CRISPRi to map cell envelope gene interactions in Bacillus subtilis, revealing >1,000 genetic interactions and uncovering gene networks in envelope biogenesis and ...
https://www.cell.com/cell-systems/fulltext/S2405-4712%2825%2900239-X
about 1 month ago
0
15
8
Bye bye Binz! It’s really hard to leave Rügen on such a fantastic morning
about 1 month ago
0
3
0
Impressions from a weekend at the Baltic Sea coast: Stralsund, Binz, Sellin, Cap Arkona
about 1 month ago
0
2
0
Very much looking fo to the PhD defense of the brain behind
#SubtiWiki
,
@c-elfmann.bsky.social
. It will take place on October 17 at 2 pm.
@imprsgs.bsky.social
about 2 months ago
1
7
0
Read a Limerick on B. subtilis on the
#SubtiWiki
homepage! Source: The Omnificent English Dictionary in Limerick Form (
oedilf.com
)!
2 months ago
0
2
1
reposted by
Jörg Stülke
Michael Galperin
2 months ago
Some thoughts on metabolism and metabolic pathway holes (PMID: 40892933)
pnas.org/doi/10.1073/...
.
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Using bioinformatics for identifying and plugging metabolic pathway holes | PNAS
Using bioinformatics for identifying and plugging metabolic pathway holes
http://pnas.org/doi/10.1073/pnas.2518071122
0
12
3
The
#SubtiWiki
Paper of the month for August 2025 has been selected. Congratulations, Marianne Broc,
@axelmagalon.bsky.social
and all involved!
pubmed.ncbi.nlm.nih.gov/40855134/
3 months ago
0
1
0
Discovery of a novel membrane plugin that links formate and NAHD dehydrogenases to the quinone pool for respiration! Work by Marianne Brock et al.,
@axelmagalon.bsky.social
and Anne Walburger lab @
@univ-amu.fr
www.nature.com/articles/s41...
#SubtiWiki
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A scaffold for quinone channeling between membrane and soluble bacterial oxidoreductases - Nature Structural & Molecular Biology
Here, the authors describe a tubular module connecting oxidoreductases to the membrane. This system, found in many microorganisms, reveals a distinct mode of membrane anchoring and an electron transfe...
https://www.nature.com/articles/s41594-025-01607-4
3 months ago
0
3
2
Professorship in Microbial Genetics @ University of Göttingen. Don't miss this chance to become my colleague!
uni-goettingen.de/en/699057.html
#SubtiWiki
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W1-TT-W2 Microbial Genetics - Georg-August-University Göttingen
Website of the Georg-August-University Göttingen
https://uni-goettingen.de/en/699057.html
3 months ago
0
19
29
New cool work from French friends and colleagues: identification of Rho termination sites in B. subtilis!
academic.oup.com/nar/article/...
#SubtiWiki
@narjournal.bsky.social
@inrae-france.bsky.social
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Comprehensive mapping of transcription terminator Rho utilization (Rut) sites across the Bacillus subtilis genome
Abstract. Recent evidence indicates that the bacterial Rho helicase regulates Bacillus subtilis gene expression in a growth-dependent manner. This regulati
https://academic.oup.com/nar/article/53/15/gkaf765/8234004
3 months ago
0
14
11
The
#SubtiWiki
paper of the month for July has been chosen! Cool work from Paris!
www.nature.com/articles/s41...
3 months ago
0
0
2
Metals are essential for life, but they can also be toxic. Here,
@johnhelmann.bsky.social
gives an excellent overview on bacterial metal physiology!
@natrevmicro.nature.com
#SubtiWiki
www.nature.com/articles/s41...
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Microbial metal physiology: ions to ecosystems - Nature Reviews Microbiology
Metal ions are required for all cells, and their homeostasis relies on ancient mechanisms that facilitate their import, distribution and storage. In this Review, Helmann discusses the key chemical con...
https://www.nature.com/articles/s41579-025-01213-7
4 months ago
0
15
4
Hello from the impressive Grundtvig’s church in Copenhagen!
4 months ago
0
3
0
News on the mechanisms of lateral peptidoglycan growth in Bacillus subtilis! @natcomms.nature.com
#SubtiWiki
4 months ago
0
0
0
Good evening from Göttingen! This car, a more than 35 year old Trabant made of plastic material, was next to mine at the petrol station this afternoon. I earned my driver‘s licence on a Trabant back in 1982!
4 months ago
0
1
0
Absolutely amazing paper from the lab of Carol Gross! So inspiring!
@biorxiv-microbiol.bsky.social
#mycoplasma
#subtiwiki
www.biorxiv.org/content/10.1...
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Predicting the protein interaction landscape of a free-living bacterium with pooled-AlphaFold3
Accurate prediction of protein complex structures by AlphaFold3 and similar programs has been successfully used to predict the presence of protein-protein interactions (PPIs), but this technique has n...
https://www.biorxiv.org/content/10.1101/2025.07.01.662654v1
4 months ago
1
8
4
Great paper on the function of SpoIIIL! Congrats to
@crodrigueslab.bsky.social
@plos.org
#SubtiWiki
journals.plos.org/plosgenetics...
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SpoIIIL is a forespore factor required for efficient cell-cell signalling during Bacillus subtilis sporulation
Author summary Bacterial endospores allow bacteria to survive stress, persist in the environment and seed new and recurring infections. Cell-cell signalling is a cornerstone of how bacteria develop in...
https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1011768
4 months ago
1
2
1
The
#SubtiWiki
Paper of the month for June 2025 has been selected. Congratulations,
@apbrogan.bsky.social
@
@harvardmed.bsky.social
@natmicrobiol.nature.com
subtiwiki.uni-goettingen.de/wiki/index.p...
5 months ago
0
6
3
Really proud and grateful that lab members (current and former) have received poster and presentation awards! Congratulations, Javi and Robert
@warneke.bsky.social
!
@uni-goettingen.de
@imprsgs.bsky.social
#subtiwiki
5 months ago
1
5
0
Don't miss the 1st Symposium on Interconnections of Gene Expression Processes this September in Göttingen! Great lineup of speakers and plenty of chances for ECRs to present & discuss their work! Link:
sfb1565.uni-goettingen.de/symposion/
@rnasociety.bsky.social
@uni-goettingen.de
#subtiwiki
5 months ago
0
3
3
@apbrogan.bsky.social
et al. show how the CdaR protein senses cell envelope defects and activates CdaA to synthesize the seccond messenger c-di-AMP in Bacillus subtilis. The increase in c-di-AMP reduces turgor and protects the cell from lysis. Congratulations!
#subtiwiki
rdcu.be/ermFn
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Cyclic-di-AMP modulates cellular turgor in response to defects in bacterial cell wall synthesis
Nature Microbiology - Brogan et al. uncover a signalling pathway in which levels of the nucleotide second messenger c-di-AMP increase in response to defects in cell wall synthesis. This regulatory...
https://rdcu.be/ermFn
5 months ago
2
9
2
Shakespeare adapted to Bacillus subtilis 🥹
@annualreviews.bsky.social
#SubtiWiki
www.annualreviews.org/content/jour...
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To Sporulate or Not to Sporulate: Developmental Checkpoints Monitoring Bacillus subtilis Sporulation | Annual Reviews
Developmental processes are carefully regulated programs that are present in multiple kingdoms of life and that generally result in cell differentiation and specialization. This regulation can be medi...
https://www.annualreviews.org/content/journals/10.1146/annurev-micro-041522-103140?utm_campaign=shareaholic&utm_medium=bluesky&utm_source=socialnetwork
5 months ago
0
3
2
reposted by
Jörg Stülke
Biology OA
6 months ago
AlphaFold predictions accelerate protein structure determination but require experimental validation. Many align with crystallographic maps, but discrepancies occur, highlighting the importance of confidence assessment & verification for accurate structural insights.
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AlphaFold predictions are valuable hypotheses and accelerate but do not replace experimental structure determination - Nature Methods
An analysis of AlphaFold protein structure predictions shows that while in many cases the predictions are highly accurate, there are also many instances where the predicted structures or parts of pred...
https://doi.org/10.1038/s41592-023-02087-4
0
15
6
All metabolites have now been assigned to categories in
#SubtiWiki
! This helps to get a rapid overview on all molecules that are Part of a pathway or that group together for other reasons, Check it out! Thank you,
@c-elfmann.bsky.social
@unigoettingen.bsky.social
@imprsgs.bsky.social
5 months ago
2
10
4
c-di-AMP formation in eukaryotic cells triggers formation of the inflammosome! Cool comment by Fabian Commichau (
@molmicrounihoh.bsky.social
) & Johannes Gibhardt!
#subtiwiki
www.nature.com/articles/s41...
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c-di-AMP is an envoy of inflammation - Nature Chemical Biology
Inflammasomes are multiprotein complexes that act as central signaling hubs by transmitting receptor-generated cues to downstream targets in the vertebrate innate immune system. New work finds that c-...
https://www.nature.com/articles/s41589-025-01931-2
6 months ago
1
4
1
News on persister cell formation in Bacillus subtilis!
@natmicrobiol.nature.com
#SubtiWiki
www.nature.com/articles/s41...
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A shared alarmone–GTP switch controls persister formation in bacteria - Nature Microbiology
Alarmone synthesis depletes GTP levels leading to a GTP-dependent switch that controls triggered, spontaneous and antibiotic-induced persister formation in Bacillus subtilis.
https://www.nature.com/articles/s41564-025-02015-6
6 months ago
0
2
1
Antisense regulation of dnaA expression in Bacillus subtilis: Checkout this article I found in PLOS Genetics:
dx.plos.org/10.1371/jour...
#SubtiWiki
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An antisense RNA regulates production of DnaA and affects sporulation in Bacillus subtilis
Author summary DnaA is the highly conserved replication initiator and transcription factor found in virtually all bacteria. The synthesis and activity of DnaA are highly regulated, and different types...
https://dx.plos.org/10.1371/journal.pgen.1011625
6 months ago
0
0
2
News on the control of potassium transport! Congratulations,
@ihaenelt.bsky.social
, JanetVonck and teams!
www.nature.com/articles/s41...
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A short intrinsically disordered region at KtrB’s N-terminus facilitates allosteric regulation of K+ channel KtrAB - Nature Communications
KtrAB is a major potassium uptake system that has been linked to the pathogenesis of many infectious bacteria. Here the authors show that KtrB from Vibrio alginolyticus contains an intrinsically disor...
https://www.nature.com/articles/s41467-025-59546-z
6 months ago
1
4
0
Happy to share Christoph Elfmann's new paper that is now out in Trends in Microbiology! Discover tools to make the most out of AlphaFold and its iterations!
@c-elfmann.bsky.social
@cp-trendsmicrobiol.bsky.social
@unigoettingen.bsky.social
@imprsgs.bsky.social
#subtiwiki
www.cell.com/trends/micro...
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Cutting-edge tools for structural biology: bringing AlphaFold to the people
The recent application of artificial intelligence-based prediction of protein and protein complex structures by AlphaFold has revolutionized biology. Here, we give an overview of the tools that make this revolution accessible to experimental microbiologists without training in bioinformatics or sophisticated computer equipment, and show how this helps to gain new insights.
https://www.cell.com/trends/microbiology/fulltext/S0966-842X%2825%2900110-6
6 months ago
1
17
6
Save the date: 6. Science Night in Göttingen! June 21 At 6.15 p.m. I will have a talk in HS001: From a minimal bacterium to vegan milk Don’t miss. I am looking forward to seeing you!
@unigoettingen.bsky.social
#subtiwiki
7 months ago
0
3
1
Breath-taking combination of cryogenic-ET, XL-MS and bioinformatics identifies a non-canonical bacterial protein translocation-folding complex! @RappsilberLab @Liang_Xue_ @TypasLab @jankosinski
www.biorxiv.org/content/10.1...
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https://www.biorxiv.org/content/10.1101/2025.04.25.650208v1.full.pdf
7 months ago
0
4
0
Learn about the conservation of genomic positions for important gene families!
@science.org
#SubtiWiki
www.science.org/doi/10.1126/...
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Most bacterial gene families are biased toward specific chromosomal positions
The arrangement of genes along bacterial chromosomes influences their expression through growth rate–dependent gene copy number changes during DNA replication. Although translation- and transcription-...
https://www.science.org/doi/10.1126/science.adm9928
7 months ago
0
2
0
Cool new features in
#SubtiWiki
: You can now do a Foldseek search with your selected B. subtilis protein against the complete Foldseek database! Moreover, Christoph has added the TED database to Outlinks. There you will see the conserved fold organization of your protein!
@c-elfmann.bsky.social
7 months ago
0
19
7
Very cool story! Congrats to all involved!
add a skeleton here at some point
7 months ago
0
2
0
Good evening from the central campus of
@unigoettingen.bsky.social
#cherryblossom
8 months ago
1
7
0
reposted by
Jörg Stülke
Andreas Peschel
8 months ago
You have a strong background in molecular microbiology and look for a PhD position? Join us in
#Tubingen
in an international, enthusiastic team team, in an excellent research environment of the EU-funded
#AUREUS
program
euraxess.ec.europa.eu/jobs/328844
@cmfi.bsky.social
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PhD student / doctoral candidate in Infection Biology
AUREUS is a multidisciplinary European Training Network built to counteract multidrug resistant Staphylococcus aures infections, funded by Marie Sklodowska-Curie Doctoral Network (MSCA-DN).
https://euraxess.ec.europa.eu/jobs/328844
0
13
11
Two out out of four PhD awards of the
@vaam-microbes.bsky.social
go to young Bacillus researchers! Congratulations, @phagefluencer,
@warneke.bsky.social
and
@molmicrounihoh.bsky.social
!
@unigoettingen.bsky.social
@unihohenheim.bsky.social
#SubtiWiki
8 months ago
1
16
0
@warneke.bsky.social
receives the PhD Award of the
@vaam-microbes.bsky.social
at the annual congress! Congratulations, Robert!
8 months ago
2
13
6
@c-elfmann.bsky.social
presents his work at the Mosbach Kolloquium!
#subtiwiki
8 months ago
0
3
0
Good evening from Greifswald!
8 months ago
0
2
0
Good evening from Tangermünde!
8 months ago
0
4
0
News on the regulation of acetyl-CoA synthesis!
@naturecomms.bsky.social
@bangebalcony.bsky.social
#SubtiWiki
www.nature.com/articles/s41...
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Regulation of acetyl-CoA biosynthesis via an intertwined acetyl-CoA synthetase/acetyltransferase complex - Nature Communications
Here the authors investigate the regulatory mechanisms of acetyl-CoA (Ac-CoA) biosynthesis in Bacillus subtilis, probing the interaction between acetyl-CoA synthetase (AcsA) and acetyltransferase (Acu...
https://www.nature.com/articles/s41467-025-57842-2#Tab1
8 months ago
0
10
6
Das Göttinger Tageblatt verbreitet (leider mal wieder) Fake News! Meinen Sie, dass die überfallene Ukraine keinen dauerhaften Frieden will??
8 months ago
0
0
0
Learn how Bacillus subtilis copes with Photo-leucine, by stopping import, activating export and by increasing the selectivity of Leu-tRNA synthetase!
@biorxiv-microbiol.bsky.social
#SubtiWiki
www.biorxiv.org/content/10.1...
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Photo-leucine incorporation in Bacillus subtilis: Identification of transporters and of a selectivity determinant in the Leu-tRNA synthetase
The elucidation of the intricate network of proteins is essential for our understanding of cellular function. In vivo crosslinking is a powerful tool for probing these interactions, with photoactivata...
https://www.biorxiv.org/content/10.1101/2025.03.07.642037v1
8 months ago
1
6
2
Great study by
@johnhelmann.bsky.social
and collaborating teams!
@naturecomms.bsky.social
#SubtiWiki
www.nature.com/articles/s41...
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Structural basis for transcription activation through cooperative recruitment of MntR - Nature Communications
MntR is a protein that can repress the expression of Mn2+ uptake transporters and activate the expression of efflux proteins. Here, the authors use cryo-EM and other biochemical approaches to establis...
https://www.nature.com/articles/s41467-025-57412-6
8 months ago
0
6
1
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