Henning Jessen (@jessenlab.bsky.social)

Happy to share Guizhen´s and Geun-Don´s analytical tour de force using 18O water labeling in combination with capillary electrophoresis Pools of Independently Cycling Inositol Phosphates Revealed by Pulse Labeling with 18O-Water | Journal of the American Chemical Society pubs.acs.org/doi/10.1021/...

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Pools of Independently Cycling Inositol Phosphates Revealed by Pulse Labeling with 18O-Water Inositol phosphates control many central processes in eukaryotic cells including nutrient availability, growth, and motility. Kinetic resolution of a key modulator of their signaling functions, the turnover of the phosphate groups on the inositol ring, has been hampered by slow uptake, high dilution, and constraining growth conditions in radioactive pulse-labeling approaches. Here, we demonstrate a rapid (seconds to minutes) and nonradioactive labeling strategy of inositol polyphosphates through 18O-water in yeast, human cells, and amoeba, which can be applied in any media. In combination with capillary electrophoresis and mass spectrometry, 18O-water labeling simultaneously dissects the in vivo phosphate group dynamics of a broad spectrum of even rare inositol phosphates. The good temporal resolution allowed us to discover vigorous phosphate group exchanges in some inositol polyphosphates and pyrophosphates, whereas others remain remarkably inert. We propose a model in which the biosynthetic pathway of inositol polyphosphates and pyrophosphates is organized in distinct, kinetically separated pools. While transfer of compounds between those pools is slow, each pool undergoes rapid internal phosphate cycling. This might enable the pools to perform distinct signaling functions while being metabolically connected. https://pubs.acs.org/doi/10.1021/jacs.4c16206