happy #fluorescencefriday Compare with last week’s post on APOE3 mice now we have the AD APOE4 model with blood vessels in pink, amyloid in blue and microglia in green. Courtesy of the @mind-western.bsky.social @touchscreencog.bsky.social @tridentpct.bsky.social teams

#fluorescencefriday brings another example of blood vessels labeled with CD31 (yellow), amyloid plaques (cyan) and microglia (IBA1 magenta) in our ApoE4 🐭 model of Alzheimer’s disease. from the @mind-western.bsky.social team 🧠 cleared with SHIELD and images by 💡 sheet 🔬

#fluorescencefriday we are back to amyloid (orange) and blood vessels (green) on a triple KI mouse line (Appnl-f, hMAPT and hApoE3). Much less amyloid in blood vessels than the correspondent ApoE4 mice. By the @mind-western.bsky.social team

#fluoresencefriday an image from a cleared brain 🧠 labeled for Choline acetyltransferase by the @mind-western.bsky.social team

Bloody brain 🧠

for #fluorescencefriday we have the dance of blood vessels by @mind-western.bsky.social @shahrzadbahrampour.bsky.social @nadiahaghbinphd.bsky.social and Vladislav Novikov

#fluorescencefriday mapping the relationship between amyloid (green), microglia (magenta) and blood vessels (orange) in and aggressive model of AD. from @arashsalah.bsky.social @nadiahaghbinphd.bsky.social @shahrzadbahrampour.bsky.social @mind-western.bsky.social

#fluorescencedriday First attempt on NeuN labeling (magenta) to quantify the 3D distribution of neurons and interface with blood vessels (yellow) by @shahrzadbahrampour.bsky.social and @nadiahaghbinphd.bsky.social for the @mind-western.bsky.social @neuroak.bsky.social

A snow day for the brain — microglia mirroring winter’s touch ❄️!

#fluorescencefriday 🧠 from AppNL-G-F mouse model triple stained for amyloid beta (Cyan) IBA1 (microglia magenta) and CD31 (blood vessels yellow) thanks to @shahrzadbahrampour.bsky.social @nadiahaghbinphd.bsky.social and @arashsalah.bsky.social @mind-western.bsky.social

It's a Happy #FluorescenceFriday when we get a personal tour of the Western Lightsheet Microscopy Core, part of @touchscreencog.bsky.social + an integral part of #TRIDENT! 🧠 🔬 Sound intriguing? See more below🤓! @mind-western.bsky.social @lifecanvastech.bsky.social #NFRF youtube.com/shorts/AA91I...

The TRIDENT proof-of-concept platform began in 2023 through the incredible support of a $24M grant from the Government of Canada's New Frontiers In Research Fund- Transformation stream. Check out our website tridentpreclinicaltrials.org or the video below 👇 #NFRF www.youtube.com/watch?v=RCr3...

#fluorescencefriday microglia (yellow) and Abeta plaques (magenta) in our AD mouse model in 3D from a cleared 🧠 using @lifecanvastech.bsky.social SHIELD Courtesy of @shahrzadbahrampour.bsky.social and @nadiahaghbinphd.bsky.social with @mind-western.bsky.social and @neuroak.bsky.social Low-res.

#fluorescencefriday shows some testing of ChAT staining in the mouse brain in cleared samples courtesy of @nadiahaghbinphd.bsky.social @mind-western.bsky.social

Going for a rollercoaster ride on the spinal cord! 🤩

Great to be in Toronto for the Second AG meeting for @tridentpct.bsky.social where our 🐁 molecular and cognition core will show what we are up to in the last 2 years facilitated by @touchscreencog.bsky.social and we will continue to learn about our organoid and marmoset models.

and another 👻 entry for #fluorescencefriday courtesy of Nadia Haghbin and Shaz

Is it candy? Nope, it is a spooky 👻 cleared mouse 🧠 used for #fluorescencefriday movies

Lightsheet Meeting Series in Oct, @mind-western.bsky.social , pleasure to host Christopher Pfeiffer-Kelly of LifeCanvas Technologies Inc. @lifecanvastech.bsky.social @braincanada.bsky.social @maximoprado.bsky.social🔬🤩🧠

#fluorescencefriday Side by side comparison of alpha synuclein S129 (magenta), a marker of #parkinson’s , in M83 hemyzigous mice injected with human pre formed fibrils @shahrzadbahrampour.bsky.social Vlad Novikov clearing with SHIELD @lifecanvastech.bsky.social imaged at MIND platform @westernu.ca

Had a great time at the @mind-western.bsky.social Lightsheet Meeting Series this week, an amazing, interactive talk led by Christopher Pfeiffer-Kelly of LifeCanvas Technologies Inc. @braincanada.bsky.social @maximoprado.bsky.social🔬🖥️🤩

Follow an exciting new brain imaging platform, @mind-western.bsky.social, partnering group of #TRIDENT. Article about it linked below! 👇🔬👀 www.schulich.uwo.ca/about/news-e...

#fluorescencefriday special. Half marmoset 🧠 cleared and stained using SHIELD and imaged by lightsheet microscopy. Courtesy of Jesleen Saini @shahrzadbahrampour.bsky.social and @stefan-everling.bsky.social

Great collaborative effort to get this done

How does the brain work? Scientists are closer to the answer with the largest wiring diagram and functional map of a mammalian brain to date. 🧵 🧠📈

It is #fluorescencefriday and the MIND platform @touchscreencog.bsky.social @tridentpct.bsky.social invites you to see brain blood vessels stained with CD31 and imaged by lightsheet. 🐁 🧠 cleared with SHIELD . thanks as always to @shahrzadbahrampour.bsky.social @arashsalah.bsky.social and company.

Hey #AAIC25 check out Arash’s presentation today at 8 AM and see some cool whole brain videos of the vasculaturs and plaques plus touchscreen testing room 106 Evaluating the Efficacy and Risks of Early Lecanemab Treatment in APOE4 Humanized Alzheimer’s Disease Mouse Models also poster 151

#FluorescenceFriday Testing a new microscope. Microglia (yellow) and S129 alpha synuclein (magenta) and nuclei (blue) spinning disc confocal image @leicamicrosystems.bsky.social

Latest paper is Vlad Novikov’s careful analysis of proteostasis in a TDP-43 mouse model A Longitudinal Study of Sex Differences in a TDP‐43 Mouse Model Reveals STI1 Regulation of TDP‐43 Proteinopathy and Motor Deficits - Journal of Neurochemistry - onlinelibrary.wiley.com/doi/10.1111/...

a second rendering for #fluorescencefriday

Our new interest: blood vessels and amyloid plaque interactions after lecanemab treatment of an ApoE4 AD mouse line #fluorescenceFriday @arashsalah.bsky.social and @shahrzadbahrampour.bsky.social for credits

Hot off the press Huge congrats to Alycia Crook a PhD student from the lab www.nature.com/articles/s41...

3D STED of PSD95-STAR RED, in cleared mouse brain tissue. 50% TauSTED 3D (left) and 100% TauSTED 3D (right), showing the difference in the axial resolution decrease from 274nm (left) to 143nm(right)! Sample prepared by Thomas Stroh group Montreal Neurological Institute (MNI). #MLMC 2025-July.

late for #fluorescenceFriday. Here are our efforts to look at blood vessels in the 🧠 and of course Abeta plaques @touchscreencog.bsky.social @tridentpct.bsky.social

#fluorescencefriday blood vessels and amyloid plaques in a 🐭 model of AD. courtesy of @shahrzadbahrampour.bsky.social Luke Smolders and Arash Salahinejad @tridentpct.bsky.social @touchscreencog.bsky.social We will be 🔜 opening up for external services and collaborations via MIND @westernu.ca

For #fluorescencefriday the Mesoscopic Integrated NeuroImaging Data (MIND) platform @westernu.bsky.social presents abeta plaques and blood vessels in the 🧠 of our ApoE4 mouse model of AD. 🧪 #clearing #lightsheet 🔬 Courtesy of Arash Salahinejad, Luke Smolders and @shahrzadbahrampour.bsky.social

#fluorescencefriday a 🧠 full of amyloid courtesy of @shahrzadbahrampour.bsky.social

#fluorescencefriday we take a look at blood vessels (yellow CD31) and amyloid (blue).

Did you miss @maximoprado.bsky.social and @mallarchak.bsky.social's presentation at #hbhlsymposium2025? You can get a glimpse of their work in this short video that capped their talk.

#fluorescencefriday takes a look at oligodendrocytes and OPCs in the cleared mouse 🧠 stained for Olig2 and imaged by #lightsheet 🔬 . By @shahrzadbahrampour.bsky.social @kesummers.bsky.social in collaboration with Corey Baron’s lab. @tridentpct.bsky.social @westernubrainscan.bsky.social

Thanks to @westernubrainscan.bsky.social and particularly Erin/Amanda for this cool video explaining the work @mallarchak.bsky.social and I plus a number of colleagues engaged with @touchscreencog.bsky.social do. With sustainability for the next few year thanks to @tridentpct.bsky.social

#fluorescencefriday M83 hemizygous 🐁 model injected with preformed alpha synuclein fibrils or vehicle (next movie) in their striatum and stained for alpha synuclein S129 (yellow) reflecting protein misfolding 🧠 cleared with SHIELD and imaged by lightsheet 🔬 1/2

#fluorescencefriday M83 hemizygous 🐁 controls nieces with vehicle 2/2

For #fluorescencefriday 👀 #lightsheet 🔬 M83 mice expressing human alpha-synuclein transgene injected with preformed fibrils of Syn in the striatum. Syn S129 pathology (yellow) propagates along neuronal pathways and follows transgene expression by Vlad Novikov and @shahrzadbahrampour.bsky.social

#fluorescencefriday. Let’s take a look at the brain vasculature with this rendered video in a whole mouse brain. 🧠 Labelled with CD31 by @shahrzadbahrampour.bsky.social

Busy tomorrow so getting some images prepared by @shahrzadbahrampour.bsky.social out for #fluorescencefriday blood vessels and abeta in vehicle and lecanemab-treated App APOE4 and tau humanized 🐁 (which I presented by zoom earlier today at #ADPD2025 )

#fluorescencefriday brings you a cleared AD mouse 🧠 stained for Abeta (green) and vasculature (magenta). Colocalization is white. #lightsheetmicroscopy 👀 for Abeta in blood vessels to test for CAA or micro bleeding. Courtesy of @shahrzadbahrampour.bsky.social Luke Smolders and Arash Salahinejad

Another weekly highLIGHT from the team at Western University, also partnering research group of TRIDENT! Take a peek, below! 👇🔬🐁🧠 #fluorescencefriday tridentpreclinicaltrials.org

#fluorescenceFriday. Lightsheet 3D movie of S129 Synuclein (magenta) and nuclear marker (green) in M83 mice injected with Synuclein preformed fibrils (also known as PFFs) in the right striatum. Spinal cord lights up because of high expression of human alpha-synuclein driven by the Prnp promoter