Lewis Holt
@lewisholt.bsky.social
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PhD candidate in the Murphy-Royal astrocyte lab. Astrocytes in stress 🏴🇪🇺🇨🇭
reposted by
Lewis Holt
Ciarán Murphy-Royal
22 days ago
Thanks Bruno Geoffroy
@crchum.bsky.social
for the nice write up on our recent publication with
@lewisholt.bsky.social
nouvelles.umontreal.ca/en/article/2...
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After early-life stress, astrocytes affect behaviour
Canadian researchers show that stress modifies the morphology of brain cells in mice, directly influencing the rodents’ level of physical activity.
https://nouvelles.umontreal.ca/en/article/2025/09/04/after-early-life-stress-astrocytes-affect-behaviour/
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Super psyched to help close out the
#AstrocyteCafe
on thursday!
add a skeleton here at some point
about 2 months ago
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Absolutely thrilled to share the first half of my PhD work! Super proud to see it finally out!
www.nature.com/articles/s41...
Thread below for a quick (10 point) recap of what we found! 🧵🧪🧠
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A divergent astrocytic response to stress alters activity patterns via distinct mechanisms in male and female mice - Nature Communications
Early-life stress has lifelong impacts on neuronal function and behavior. Here, the authors show that astrocytic glucocorticoid receptor signaling drives stress-induced neuronal and behavioral change ...
https://www.nature.com/articles/s41467-025-61643-y
3 months ago
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reposted by
Lewis Holt
Ciarán Murphy-Royal
3 months ago
Final leg of the Tour de France done with an excellent
@gliameeting.bsky.social
! Amazing science in a great city, (despite some smoke!) and a nice travel award for
@lewisholt.bsky.social
🥳 Going to make sure we come back to this meeting again next time!
#glia2025
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Was great to present at
@canglia.bsky.social
today! Cool to see all the glial research happening across Canada!
add a skeleton here at some point
4 months ago
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reposted by
Lewis Holt
Alex Chen
4 months ago
Excited to share that the astrocyte-centered half of my thesis work is now out
@science.org
! With
@mishaahrens.bsky.social
and
@marcduque.bsky.social
science.org/doi/10.1126/...
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Norepinephrine changes behavioral state through astroglial purinergic signaling
Both neurons and glia communicate through diffusible neuromodulators; however, how neuron-glial interactions in such neuromodulatory networks influence circuit computation and behavior is unclear. Dur...
https://science.org/doi/10.1126/science.adq5233
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reposted by
Lewis Holt
Papouin Lab @ WashU
4 months ago
Thought this would be a nice way to enter the bsky community👇Delighted for Katie (now in Chris Dulla's lab at Tufts). Thank you to our collaborators, to the reviewers and to Cagla Eroglu for the highlight!
www.science.org/doi/10.1126/...
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Norepinephrine signals through astrocytes to modulate synapses
Locus ceruleus (LC)–derived norepinephrine (NE) drives network and behavioral adaptations to environmental saliencies by reconfiguring circuit functional connectivity, but the underlying synapse-level...
https://www.science.org/doi/10.1126/science.adq5480
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Super cool that these are finally out!! Check out some of the protocols we've been using to investigate the role of astrocytes in stress!
add a skeleton here at some point
6 months ago
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Multinucleated astrocytes always look so cool!
#FluorescenceFriday
6 months ago
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Diversifying my portfolio with some Iba-1 staining.
#fluorescentfriday
7 months ago
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reposted by
Lewis Holt
Ciarán Murphy-Royal
8 months ago
Version of Record of our work at
@elife.bsky.social
has been published! Super proud of this one after all the hard work of the lab seeing it to the end. Make sure to click through figure supplements as there is so much good stuff in there - sex differences, etc.
elifesciences.org/articles/99988
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Early-life stress induces persistent astrocyte dysfunction associated with fear generalisation
Amygdala astrocytes play a critical role in mediating the effects of stress during infancy on neuronal excitability, synaptic plasticity, and memory.
https://elifesciences.org/articles/99988
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technical question. Can anyone recommend a good commercially available SOX9 antibody for fixed tissue IHC?
#astrosky
#astrocytes
8 months ago
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reposted by
Lewis Holt
Mackenzie Weygandt Mathis
9 months ago
What a year!
#DLC3🔥
is up tested by many of you (👀 watch out for a paper in 2025), we hit 750K downloads, and have over 120 contributors to the code!
@deeplabcut.bsky.social
❄️⛷just dropped: new human pose models & high perf. models!
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reposted by
Lewis Holt
Ciarán Murphy-Royal
10 months ago
Delighted to share an update to our preprint, reviewed at
@elife.bsky.social
, and now sent back for re-review. Colossal work by the team and driven home by
@mathiasgua.bsky.social
Quick 🧵 of what's new -
www.biorxiv.org/content/10.1...
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Early-life stress induces persistent astrocyte dysfunction associated with fear generalisation
Early-life stress can have lifelong consequences, enhancing stress susceptibility and resulting in behavioural and cognitive deficits. While the effects of early-life stress on neuronal function have ...
https://www.biorxiv.org/content/10.1101/2022.12.11.519598v4
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reposted by
Lewis Holt
Liisa Galea
10 months ago
Our latest review-with
@bonniee-leee.bsky.social
where we pour our ❤️🧠 & soul to outline our position on why we are so passionate about harnessing the power of sex differences & within sex diff to propel precision medicine forward let us know if we convince you
www.nature.com/articles/s41...
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Leveraging research into sex differences and steroid hormones to improve brain health - Nature Reviews Endocrinology
This Review outlines how the effects of steroid hormones on hippocampal neuroplasticity across the adult lifespan differ between the sexes, highlighting the importance of considering sex and steroid h...
https://www.nature.com/articles/s41574-024-01061-0
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reposted by
Lewis Holt
Shane Liddelow
10 months ago
There are lots of us missing, but so many joining so quickly Please do share, and comment below to be added
go.bsky.app/5f2V6L6
(microglia starter pack also here by
@mancusorenzo.bsky.social
-
bsky.app/starter-pack...
)
add a skeleton here at some point
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Not sure I have any good pictures in the back pocket to beat the calcium imaging from
@mathiasgua.bsky.social
Instead for my first post on Bluesky I want to share the CANglia webinar: trainee series!
10 months ago
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