Had a couple of good questions tonight: Dad, what would happen if you threw a gallon of water on the sun? What would happen if a planet that was 700 degrees hot crashed into a planet that was 700 degrees below zero?

Brief, sunny NYC trip, now to Boston for a few days

Clever “cell floating assay” using CellTrace Violet to sort binders: De novo design of miniprotein agonists and antagonists targeting G protein-coupled receptors www.biorxiv.org/content/10.1...

Good afternoon for a jog past the @uoknightcampus.bsky.social

T shirt and sunglasses day on the mountain

Spring skiing on Mt Hood

🌷Spring flowers? Fireworks? Mouse cerebral cortex FFPE tissue was labeled with eight primary antibodies. Aluora Spatial Amplification Kits and DAPI were used for detection, followed by imaging on the EVOS S1000 Spatial Imaging System. 🧠🔬🧪 #Neuroscience #FluorescenceFriday

Some microscopy art for St. Patrick's Day. This is antibody purification resin after cleaning up an Alexa Fluor 488 antibody conjugate. 🧪

Three feet of powder means fun in the trees ❄️

For #FluorescenceFriday: a very thin slice of rat kidney was labeled with 8 primary antibodies whose signal was amplified with Aluora Spatial Amplification reagents. 🔬 on the EVOS S1000 Spatial Imaging system. >400 merged fields of view at 20x.

🧬A new imaging technique developed by the Liu Lab uses a novel DNA barcode system to track hundreds of RNA & protein molecules in single cells within thick biological samples, providing a full picture of how these structures are organized inside tissues. 🔗 www.janelia.org/news/new-met...

Mountain biking and skiing in the same weekend 🤘

Some hummingbirds appear colorful due to iridescence caused by the microscopic structure of their feathers. Here’s an Anna’s hummingbird feather from my yard at 460x 🧪

Under normal light fluorophore colors appear inverted: red dyes look blue, blue appear red, and green dyes are purple. Using an inverse LUT shows us this same palette on our microscopy sample. 🧪

Here's a set of three for #FluorescenceFriday. Same image, progressively higher zoom. FFPE human tonsil tissue labeled with primary antibodies against Cytokeratin, PCNA, C20, CD45RB, CD68, eCadherin, and SMA. 🧪🔬

Today in @nature.com: remarkable extended survival in pancreatic cancer patients treated with cancer vaccine. Autogene cevumeran induces CD8+ T cells with est. lifespan of 7.7 years (range 1.5 to roughly 100 years), with 20% of clones that may outlive hosts. www.nature.com/articles/s41...

The wetlands outside my office window often has nutria and geese. Today it was a pair of foxes. 🦊

FDA approves TROP2-targeted antibody–drug conjugate for breast cancer www.nature.com/articles/d41... Daiichi Sankyo and AstraZeneca’s new ADC datopotamab deruxtecan will compete with Gilead/Immunomedics’ sacituzumab govitecan, which is expected to have sales of ~$1.7 billion in 2025

🦠🔬 Recently we reported the development of a new class of sensors to directly monitor live-cell protein trafficking and provide a sensitive indication of delivery to functional, enzymatically active lysosomes. A quick 🧵for #FluorescenceFriday www.biorxiv.org/content/10.1...

Fascinating deep-dive into Roche’s new Sequencing By Expansion (SBX) technology omicsomics.blogspot.com/2025/02/roch...

Deep-tissue transcriptomics and subcellular imaging at high spatial resolution | Science www.science.org/doi/10.1126/...

A quick photo of lab equipment at 400x: here’s some antibody purification resin after conjugating trastuzumab with Alexa Fluor 594.

📢 Submissions are now open for the CellMap Segmentation Challenge. Build the best method for segmenting cellular organelles in vEM using our 289 annotated volumes, 40 organelle classes, and 22 diverse cell and tissue types. 🔗 hhmi.news/410evJQ

Dropping another one for #FluorescenceFriday #Valentine's. FFPE Human Duodenum Tissue, EVOS M7000 20X. Primary antibody staining for P53, SMA, PCNA, EpCam, HLA-DR plus DAPI.

It's an exciting day: Valentine's, #FluorescenceFriday, and new product launch day. Our upgraded Alexa Fluor Antibody Labeling kits now feature pre-assembled, pre-filled spin columns, shaving off a full hour from each conjugation. Plus, they are now compatible with any microcentrifuge.

To accompany the Biologists @ 100 conference, we're launching an image competition together with @focalplane.bsky.social! Enter your best biological research images 🧪🔬 Shortlisted images will be presented at the #Biologists100 conference in March. Details: thenode.biologists.com/enter-the-no...

Monitoring cellular uptake and lysosomal catabolism of proteins is fundamental to development of antibody-drug conjugates and targeted protein degraders. Here an anti-HER2 IgG labeled with LysoLight Deep Red displays bright fluorescence only upon delivery to the lysosome. 66hr time lapse.

The unique pharmacology of targeted degraders (eg PROTACs) leverages their sub-stoichiometric “catalytic” activity. Now ⁦‪Lycia Tx‬⁩ reports catalytic (cata)LYTACs for potent, durable extracellular #TPD, cleared IgE in NHPs, superior to omalizumab 👏 www.biorxiv.org/content/10.1...

12,000 miles in the air this week 🛫 - time for some miles on the running shoes 👟

🧵Prepint alert! Optimizing Multifunctional Fluorescent Ligands for Intracellular Labeling | tinyurl.com/3n55hvsc. With Jason Vevea, Ed Chapman, and @so-lets-kilab70.bsky.social, we combined dye chemistry, HaloTag, microscopy and cell biology to make protein purification and manipulation tools.

Snowy walk to give the first seminar of the day in Cambridge

Try this on your next flight: Wear AirPods with noise cancellation enabled. The noise suppression is so good that you can hear each plane that passes nearby. Sounds like a small sonic boom.

Recharging this week in Maui but I’ll be in Boston next week if anyone wants to catch up

Standard weekend as a mountain bike coach

Perfect January day on the hill

In a great collaboration with @hummerlab.bsky.social and the Kräusslich lab: HIV capsid doesn't break at the NPC; instead, it cracks open the NPC itself! Details in Cell: authors.elsevier.com/sd/article/S... @mpibp.bsky.social @uniheidelberg.bsky.social A thread below:

Probably not going into lab today; wearing the lab socks anyway. 🔬

TGIF and happy #fluorescencefriday. Today it's FFPE human duodenum at 20x. 🔵Alexa Fluor 532 SMA 🟢Alexa Fluor 568 HLA-DR 🟣Alexa Fluor 647 PCNA 🔴Alexa Fluor 790 EpCAM

Amazing new work from the Baker lab: de novo design of proteins as snake venom neutralizers, but the approach is relevant to many applications. www.nature.com/articles/s41...

9-plex human tonsil 20x

Hey #CellBio2024! San Diego is beautiful, but you know what's also beautiful? SCIENCE See some beautiful science art at the @beatascienceart.bsky.social show tomorrow, including pieces from our own scientists! 🔗 beatascienceart.com/artshow2024/

This #FluorescenceFriday we’re launching a new sensor: LysoLight Green.

Just launched: 🔬 ReadyLabel™ Antibody Labeling Kits with dyes optimized for 9-plex spectral unmixing to overcome secondary detection limits for multiplex and spatial imaging www.thermofisher.com/order/catalog/product/R10714

Two exciting events happening today in Seattle: 1. The ominous bomb cyclone quickly approaching 2. Spatial biology now featuring 9-plex imaging with ReadyLabel Antibody Labeling kits (launching today!) lnkd.in/gm2XSNp2

Early-stage zebrafish embryo. Credit to Dr. Jake Leyhr. #ZebrafishZunday 🧪

Kicking off the science with an image of breast cancer cells labeled with MitoTracker, Hoechst, and a new green sensor launching soon. Can you guess what structure is labeled?

How exciting to make the jump to a new platform! Please reach out if you’re working with fluorescence, cell biology, protein engineering, or especially biotherapeutics.