Happy to share our newest preprint on antigen presentation of Mtb-derived peptides on MHC-I: www.biorxiv.org/cgi/content/... This is a follow up to our previous paper where we applied immunopeptidomics to understand MHC-I presentation by Mtb-infected cells: elifesciences.org/articles/84070

Excited to share our lab's newest preprint (strap in if you're excited about metabolism, species differences, or Mtb gene expression): www.biorxiv.org/content/10.1...

Excited to share our newest manuscript on antigen discovery and vaccine design for tuberculosis lead by Owen Leddy (everyone hiring new faculty in a few years, remember this name!) www.science.org/doi/10.1126/... #TBsky

Congrats to the Carpenter Lab on this exciting study! I might now sound like a broken record, but check the abstract!!! Let’s go type 7 secretion system substrates! #tuberculosis #tbsky

Happy to see more data pointing to an important role for type 7 secretion substrates in TB protective immunity

New video summary 🎥 from Leddy et al. @thebrysonlab.bsky.social (rupress.org/jem/article/... ) on a new workflow called PathMHC that helps find pathogen-derived antigens presented on MHCs by infected cells that could be targets for T cell priming vaccines:

Newest paper from the lab out today (doi.org/10.1084/jem....)! Here, we describe PathMHC, a novel MS-based approach to identify rare pathogen-derived peptides on MHC-I and MHC-II. We apply this to a range of pathogens Mtb, SARS-CoV-2, and Listeria.

Anyone have experience with IV mRNA-LNP vaccination in animals? I am specifically interested in LNP formulations and what the preferred formulation for IV is.

Pictured: glass of champagne I treated myself to at DCA upon learning the lab has been tenured! Grateful to my lab family, my extended science family, and my family for the amazing support and love offered on the journey so far. Much more work left to do but taking this moment to say thank you!

Does anyone know of a CRO that produces MVA-based vaccines?

Do any of my #tbsky friends have interests in T cell responses in people with evidence of prior Mtb infection? If so, would you have any interest in looking at T cell responses to Mtb-derived peptides that we have detected by mass spectrometry? LMK 🙏🏽

First post here! To inaugurate this account, I'd like to share my recent work, now published in JACS. Intercepting a Mycobacterial Biosynthetic Pathway with Covalent Labeling | Journal of the American Chemical Society pubs.acs.org/doi/10.1021/...

Does anyone have a favorite protocol for in vitro priming of human T cells from PBMCs? #immunosky

So glad to see this out! I learned a lot in this project about how to think creatively about customizing our approach to using language models for immune receptors

Immunopeptidomics (impep) friends, have you ever done impep to ID peptides after soluble protein delivery to phagocytes to look at peptides generated by XP?

Before I write my own analysis script, does anyone in #tbsky have a tool for pathway analysis for Mtb genes?

A lovely day to celebrate the amazing people I work with every day

What is your favorite cytosolic pathogen that (a) infects macrophages and (b) is genetically tractable? Looking for someone who’d like to team up on a small but impactful project (we would do the heavy lifting!)

My whole scientific identity over the last 96 hours has been species differences in immunity. Would love to connect with others on this topic!

Anyone on here love cathepsins or other lysosomal proteases? Would love to pick your brain for 15 minutes!

Anyone want to discuss T cell vaccines? I’ve been in a rabbit hole lately and would love to discuss with others!

I made a Starter Pack for the Myco & TB community. Hope to find more good friends here on Bluesky to keep in touch about mycobacterial infections. Please join and repost! go.bsky.app/3nwpcVV

Looking forward to spending more time on here! Tuberculosis, macrophages, antigen presentation, and systems biology folks — say hey!

#Immunology friends, what are your favorite fix/perm conditions for imaging to access antigens in the phagosomal lumen?

Looking forward to building community on here! Just saying hello for now! Bioengineer at MIT and the Ragon Institute with an interest in tuberculosis, innate immunity, and computational biology! #HiSciSky #HiImmunoSky