Niels Keijzer (@nkeijzer.bsky.social)

I'm very proud that our paper, and with that the main project of my PhD, has just been published by @natcomms.nature.com! 🎉 Here, we reveal the unique, molecular mechanism by which USP1/UAF1 cleaves ubiquitin chains on PCNA, which may direct DNA damage tolerance. doi.org/10.1038/s414...

loading . . .

USP1/UAF1 targets polyubiquitinated PCNA with an exo-cleavage mechanism that can temporarily enrich for monoubiquitinated PCNA - Nature Communications DNA damage tolerance is regulated by ubiquitination of PCNA. Here, the authors present kinetic and structural studies showing that USP1/UAF1 prefers trimming K63- and K48-ubiquitin chains down over cl... https://doi.org/10.1038/s41467-025-61768-0