It’s out! Using cryo-ET in Dicty cells, we take a fresh in situ look at vaults. Surprisingly, we uncover vaults associated with ER and NE membranes, and find that many vaults enclose ribosomes in defined orientations, opening new avenues to their cellular function! www.biorxiv.org/lookup/conte...

Just published! We share an open-source workflow to measure membrane thickness from tomograms, including a tutorial with 3D visualizations. We analyze thickness variations across organelles and reflect on where to define a membrane boundary. @becklab.bsky.social #teamtomo rupress.org/jcb/article/...

Fantastic new work from our researchers! Check out their work on 🐯 cryoTIGER for cryoET 🐯

New from my colleagues Markus Schreiber & Beata Turoňová: TANGO, an #opensource tool for more holistic spatial analysis of cryo-ET particles. It can clean or cluster particles, analyze lattices, and identify patterns. #TeamTomo www.biorxiv.org/content/10.1...

Excited and honored to speak at the @royalmicrosoc.bsky.social meeting tomorrow where I'll talk about our latest AI tools. Meanwhile, check out Vijay&Arlo's Napari "bootstrapper" plugin for massively accelerated segmentation of multidimensional imaging datasets Github: github.com/ucsdmanorlab...

In situ evidence for systematic membrane thickness variation across cellular organelles https://www.biorxiv.org/content/10.1101/2025.03.13.642912v1

Automated Removal of Corrupted Tilts in Cryo-Electron Tomography https://www.biorxiv.org/content/10.1101/2025.03.13.642992v1