www.golocalprov.com/business/nab... #nabsys

Throughout my entire Canadian Military Career I served alongside USAF personnel daily. We were a team, brothers and sisters in support of one goal, maintaining North American air sovereignty. More than colleagues, we were best friends, I bet many feel the same to this day. This all day Long 👇

I showed this graph in class yesterday on how measles cases in the United States plummeted dramatically after the vaccine was licensed in 1963. It’s not subtle and I could not resist observing: “This stunning design in measles cases was not thanks to vitamin A. It was not due to beef tallow.”

This is an excellent summary of current circumstances Kyle Kulinski: “A sundowning president with Alzheimer’s who’s a malignant narcissist is dragging us into WWIII, committing war crimes on a daily basis and crashing the global economy, and nobody is actually grabbing the steering wheel.”

Tens of thousands marching in Providence today. No kings.

Spread this dear ones: "Bernie Sanders is suggesting the Epstein files be read during the filibuster on the SAVE Act this week. Let's get this to TREND! Answered that for you

Nabsys: Snow or rain in the forecast? We’ve got you covered - literally. Stop by our booth #2007 and grab an umbrella before heading out! 👉 Visit us on the trade show floor or book a meeting with us: hubs.ly/Q046BKDj0 #ACMG #ACMG26 #ACMGMtg26 #GenomicResearch #EGM

This would be a crime against humanity, but otherwise I agree. .

> @covie93.bsky.social

will be back at same site in Orlando next year, March 1-4, 2027 #AGBTGM26

Beth Shapiro and Len Pennacchio to make closing remarks. That's it! Thanks for coming. Any feedback? #AGBTGM26

JO: DIstinct genomic compartmentalization of hypo- and hyper-methylation in cancer. Tumors share methylation states across distinct lineages. May be able to subtype cancers, use for liquid biopsy for tumor origin, identify cancer specific regulatory alterations #AGBTGM26

JO: Are there conserved methylation across cancers or cancer-type profiles? Contaminating cell types can obscure patterns. Can deconvolve cell types. #AGBTGM26

JO: see focal hypermethylation and global hypomethylation. Cancer atlas has data on 450K sites, only 1-2% fo methylome. Can now do whole methyome. Looked at 106 tumors from 21 cancer types #AGBTGM26

James Occean from Stanford will discuss whole genome profiling DNA methylation in primary tumors. Epigenetic alterations extensive in cancer #AGBTGM26

YM: longer reads can be better assigned to maternal/paternal allele to see imprinting #AGBTGM26

YM: use 5' kit and do long read 1. See extra cap G not genomically encoded in reads if goes all the way to 5' end. Do not see with premature termination to remove noise. See major start site but can also now see minor start sites previously hidden in noise #AGBTGM26

Yasuhiro Murakawa from Kyoto talking about spatial transcriptomics technology for transcription start site profiling #AGBTGM26

JB: another patient has main outlier at AS3MT, implicated in broad set of neuropsychiatric disorders. Depletion leads to increase in nerve, brain and muscle transcripts #AGBTGM26

JB: Use CRISPR guides to remove highly expressed RNA. 400,000 guides made. See highly expressed genes go down, poorly expressed go up. Uncover novel splice sites as in KCNJ6 only identified in depletion #AGBTGM26

JB: Of those with rare disease tested, 25-50% get a causal variant. RNAseq used to help interpret DNA mutations #AGBTGM26

Jon Bezney from Stanford will talk about rare disease diagnostics especially using RNAseq #AGBTGM26

EK: you can sign up your pet for pets on the ark. Free to sign up, $150 if want to have sequenced #AGBTGM26

EK: Are cats domestic? Yes, 4000 yrs ago: Evolution in response to an anthropogenic niche. A domestic population requires acces to that niche. #AGBTGM26

EK: Panagram counts kmers to compare genomes. very fast way to compare similarity. Highly correlated with Minimap #AGBTGM26

EK: Can see trait effects on GWAS Howling associated with SCN3A. See many loci associated dog height. Very heretible. #AGBTGM26

EK: 80% of current dogs are free roaming, living off human garbage just like 20,000 years ago. Dog breeds are only 150 years old. (Dirty secret: EK has never owned a dog). Behavioral traits are not fixed in breeds. Variability within breeds exceeds variability across breeds. #AGBTGM26

Elinor Karlsson from UMass Med will talk about pet genomics due to ability to do longitudinal studies, detailed phenotypes, large populations and people love to tell you about their dogs #AGBTGM26

JB: Transcription does not restart after mitosis in RanGAP1 depleted nuclei. After first indiscriminate folding program, get second folding program. Without second program, TADs and Cohesin-mediated loops do not form #AGBTGM26

JD: Folded state of genome is the sum of many different processes working simultaneously. Driven solely by chromosome-intrinsic factors. See indiscriminate clustering of small active domains (cell type specific) #AGBTGM26

JD: chromosome folding very different in interphase vs mitotic cell. Compartmentalizaiton: Hetero- and eu- chromatin spatially segregated. Chromatin looping: fast looping of DNA #AGBTGM26

Job Dekker from UMass Med to talk on mitotic inheritance of chromosome folding programs. Battle in all your cells between protein machines to organize mitotic chromosomes. #AGBTGM26

Tim Steiert form Kiel Inst Clinical Molec Biol. Trying to get more accurate blood genotyping. RHD/RHCE are too homologous to distinguish easily. Using constellation, Pacbio, and ONT to look at neighboring regions #AGBTGM26

Huy Nguyen (Bruker) look at glioblastoma tissue with 419 plex paintscape panel. Look for LOH in chr10. Looked at MET gene region with OncoPaint panel, can see MET amplification #AGBTGM26

Robert Sebra (mount Sinai) high grade serous ovarian cancer metastasis through single cell and spatial profiling. Look at median entropy. stratifies epithelial cells #AGBTGM26

Michal Lipinski (Broad) peripubal testis development profiled using spatial tech. Looked at how cells interact with neighbors. #AGBTGM26

for Kerstin Lindblad-Toh who is snowed in. Systematic discovery of non-codng driver mutations in constrained region for pan-cancer. NCCN Genes do not have coding variants so seems to be caused by amounts because proteins too critical to be knocked out #AGBTGM26

Rishona Lavy (Rady) looking at genome vs environment of Sudden unexpected infant death. Look at both together. Model how they interact. 83% of deaths have preventative measures that could be taken. #AGBTGM26

Brendan Keating (NYU) Heart allografts - precious with high rejection rates. Can we make it better? Use proteomics to look at pathways #AGBTGM26

Dimitra Karagkouni (Harvard Med) on single cell mapping of TCR. Map tumor antigen landscape to T cell recognition at single cell resolution. See cryptic antigens in multiple myeloma #AGBTGM26

Flash talks starting with Tom van der Valk on genomic and dietary insights into capercaillie using low coverage sequencing. #AGBTGM26

TMB: Have data for chimps, bonobo, orangutans, and gorillas for geolocation #AGBTGM26

TMB: Illegal trade in great apes - babies are trafficked but average of 10 adults killed in process of each one captured. To understand where an animal came from, look at feces from different areas. Looked at 828 fecal samples from 46 locations. Can now geolocate origin of rescued animals. #AGBTGM26

TMB: Getting more genomes from more species - will be able to do population genetics. Hard to get genomes but much harder to get functional information. Look at epigenetic and splicing information. Set up CRYOZOO with cell lines from across animal kingdom, making iPSCs #AGBTGM26

TMB: Primate common missense variants nearly completely exclusive of Clinvar pathogenic variants. Better predictor than more distant species. #AGBTGM26

TMB: Made first nuclear phylogeny of primate radiation. Some species do not match pre-existing taxonomy based on structure/traits. Generate human-specific AA mutations. Some previously thought to be human-only are not. #AGBTGM26

TMB: Generated more than 800 primate genomes including all families, 95% of genera and 60% of species. 33 species with more than 5 individuals. 100 species with more than 1 individual. #AGBTGM26

Tomas Marques-Bonet from Univ Pompeu Fabra to talk about primate genomes. Why primates? Can get lots of samples and make changes. #AGBTGM26

DR: periods of intense change then quiet. #AGBTGM26

DR: Can sort out connections looking at synteny that are obscured in fossil record that has gaps. Can we use knowledge of gene history to understand phenotypes? #AGBTGM26