Announcing our new preprint! We built SPICE, a framework that combines large-scale experiments and generative AI to design RNA sequences that control cell type-specific gene expression using alternative splicing – a powerful, underexplored modality. Preprint: www.biorxiv.org/content/10.1... (1/10)

Thank you to the award committee, my PI Fei Chen and my lab, all my mentors, collaborators, family and friends for your support!

1/ In two back-to-back papers, we present our de novo TRACeR platform for targeting MHC-I and MHC-II antigens TRACeR for MHC-I: go.nature.com/4gcLzn5 TRACeR for MHC-II: go.nature.com/4gj5OQk

So at the beginning of my postdoc I struggled to amplify Chlamy genes (easily 75%+ GC) and did pretty much the same comparison and found the same results DMSO= helps but kills pol processivity Betaine= Lots of spurious products 1,2-propandiol= processivity retained and usually specific product

I took biochem in 2001, and for nearly 20 years read amino acid sequences daily… and I never knew Dayhoff named them or even the logic behind things like Q until last Friday (h/t Mike Janech). Also, this is another big Dayhoff moment for me. She was incredible! #proteomics #bioinformatics

Did you know there’s a site to search for starter packs of people to follow? Built by @mubashariqbal.com blueskydirectory.com/starter-pack...

Congratulations to the team for this work! An innovative way to use #CRISPR 🧬 ! This study uses CRISPR-gRNA to barcode small extracellular vesicles, enabling subpopulation-specific analysis of their biogenesis and release. A groundbreaking tool for studying sEV heterogeneity! #MolecularBiology

Here’s my spreadsheet of starter packs (>80!) related to broadly ‘mechanistic biology’ plus some intriguing extras Complete with collective nouns I’ve been tracking these but now can’t keep up Hope it’s helpful 2/2 docs.google.com/spreadsheets...