Oli Hale
@olijhale.bsky.social
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📥 73
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Infrequently posting cycling, science and wildlife stuff.
pinned post!
The last bit of peel 👀 Simultaneously the least and most important part of installation.
#TeamMassSpec
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about 1 year ago
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reposted by
Oli Hale
BioMassSpec
22 days ago
Integrated Native Mass Spectrometry Imaging of Soluble and Membrane Proteins
#JACS
pubs.acs.org/doi/10.1021/...
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Integrated Native Mass Spectrometry Imaging of Soluble and Membrane Proteins
Native ambient mass spectrometry enables the analysis of intact protein complexes directly from fresh frozen tissue sections together with visualization of their spatial distribution as part of a mass spectrometry imaging workflow. Native mass spectrometry imaging typically employs nanospray-desorption electrospray ionization (nano-DESI), a liquid junction sampling approach. Imaging of both soluble and membrane proteins has been demonstrated by native nano-DESI but, crucially, imaging of one protein type has always been at the expense of the other, requiring tailored sample preparation and multiple tissue sections. Here, we introduce a new mode of nano-DESI operation that combines soluble and membrane protein analysis into a single experiment, requiring no sample preparation and only a single tissue section, and which is compatible with mass spectrometry imaging. Chromatography-like separation of soluble and membrane protein signals, observed as varying elution profiles, occurs when the nano-DESI probe is parked in a fixed location on the tissue. The elution profiles of proteins in both kidney and brain tissue were explored. The results show that elution profiles are quick to record, offer insight into the classification of unknown proteins detected from tissue and enable signal-to-noise improvements to imaging and native top-down mass spectrometry workflows.
https://pubs.acs.org/doi/10.1021/jacs.5c16821
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This is astonishing news. Welshpool and the surrounding area is mega
#LeTour2027
24 days ago
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Nominations for the
#EarlyCareerResearchers
(ECR) Committee of the Consortium for Top-Down Proteomics for the 2026-2028 term are open. We encourage nominations of motivated early-career researchers (including self-nominations) who are passionate about advancing top-down proteomics.
#teammassspec
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Nomination for the Early Career Researchers (ECR) Committee (term: 2026-2028) of the Consortium for Top-Down Proteomics
Open to: academia, industry, and government Criteria: (1) PhD scientist, (2) Within 10 years of graduation, (3) Actively involved in the field of intact protein analysis by mass spectrometry (broadly ...
https://docs.google.com/forms/d/e/1FAIpQLSdxQd87Yurmi45lRu1bH4hRJ5CErar3cvywVigdbvmI6t8iaQ/viewform
30 days ago
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Thanks for the fun write-up
@proteomicsnews.bsky.social
! Can confirm, all done with a home-built ion source attached to an Orbitrap Ascend. Only mouse and rat tissue here though.
proteomicsnews.blogspot.com/2025/12/nano...
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NanoDESI allows spatial intact protein complex analysis!?!
Well...this one looks like magic... I was reading it on my phone yesterday, but I'm reasonably sure this was a custom Nano DESI source equ...
https://proteomicsnews.blogspot.com/2025/12/nanodesi-allows-spatial-intact-protein.html?m=1
about 2 months ago
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My latest paper is out in Analytical Chemistry (open-access):
pubs.acs.org/doi/10.1021/...
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Imaging of Protein Assemblies up to 231 kDa in Tissues with Nano-DESI Mass Spectrometry
Understanding the distribution of proteins and their assemblies in tissues is a major challenge in spatial biology. Mass spectrometry imaging (MSI) with nanospray-desorption electrospray ionization (n...
https://pubs.acs.org/doi/10.1021/acs.analchem.5c05767
about 2 months ago
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reposted by
Oli Hale
BioMassSpec
about 2 months ago
Imaging of Protein Assemblies up to 231 kDa in Tissues with Nano-DESI Mass Spectrometry
#AC
pubs.acs.org/doi/10.1021/...
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Imaging of Protein Assemblies up to 231 kDa in Tissues with Nano-DESI Mass Spectrometry
Understanding the distribution of proteins and their assemblies in tissues is a major challenge in spatial biology. Mass spectrometry imaging (MSI) with nanospray-desorption electrospray ionization (nano-DESI) has previously enabled detection, imaging, and identification of intact protein complexes directly from tissues, including protein assemblies and pathological protein–metal complexes in neurodegenerative disease. To date, nano-DESI MSI has been most effective for lower molecular weight (MW) complexes (<100 kDa), with an upper limit of 113 kDa. Here, we demonstrate nano-DESI at molecular weights up to 231 kDa, more than doubling the previous limit, by combining nano-DESI with a new mass spectrometer system architecture designed for higher MW analysis. Both mouse brain and rat kidney tissues were analyzed. Importantly, protein identification by native top-down MS was performed exclusively by use of nano-DESI. That is, complementary techniques for protein identification, such as liquid extraction surface analysis, were not necessary. Both homo- and heteromeric proteoform assemblies were identified in complex with endogenous small-molecule and metal ion cofactors. The developments lead the way to the analysis of larger oligomeric protein assemblies and protein complexes, cementing nano-DESI as a tool for structural biology, and with implications for molecular pathology and drug discovery.
https://pubs.acs.org/doi/10.1021/acs.analchem.5c05767
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reposted by
Oli Hale
Consortium for Top-Down Proteomics
2 months ago
Join us December 11 for Jared Shaw's presentation at Proteoform Thursday: "Ribosome Heterogeneity Revealed by Complex-Up Native Mass Spectrometry and Top-Down Proteomics"
us06web.zoom.us/meeting/regi...
#proteoforms
#proteomics
#massspec
@ge-lab-uw.bsky.social
@joelooucla.bsky.social
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Exciting to see the MSn space hotting up from so many angles, research labs and vendors at the moment. New from Sciex.
pubs.acs.org/doi/10.1021/...
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Multistage Tandem Mass Spectrometry Using an Electron-Activated Dissociation Device with a Linear Ion Trap and Structural Identification of Cardiolipins
We have developed an electron-activated dissociation (EAD) device with product isolation functionality for multistage tandem mass spectrometry (MSn). The EAD portion is a branched magneto-radio frequency ion trap with an electron beam source, and we attached a linear radio frequency quadrupole (RFQ) ion trap (or D trap) to one of the branches of the EAD device. Because the D trap was installed inside a strong ring permanent magnet, we made the linear quadrupole rods from para-magnetic stainless steel, which works as a magnetic shield. Using this D trap, we isolated a fragment produced by the first dissociation technique, which can be further fragmented by collision-induced dissociation (CID) or EAD. Using the new EAD-D trap, we demonstrated a near-complete structural identification of cardiolipins via an MS3 workflow with CID followed by electronically excited dissociation (EED) after isolation from the CID products. The number of double bonds, their positions, and cis/trans isomerism in each acyl group, as well as the regioisomerism of acyl chains, were fully identified; however, the only remaining structural ambiguity of the cardiolipins was the chirality at the central carbon of the central glycerol group. We identified 18 cardiolipins in Escherichia coli (E. coli) extract.
https://pubs.acs.org/doi/10.1021/acs.analchem.5c05400
3 months ago
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Fun day out at Waters charge detection mass spectrometry (CD-MS) technology day.
3 months ago
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Really interesting work.
add a skeleton here at some point
5 months ago
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Beautiful start to May in the UK midlands ☀️☀️☀️
9 months ago
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I found a nice sheep today.
about 1 year ago
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Gooey gravel ride on a sunny January day. Spring soon 🌱🌼
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about 1 year ago
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reposted by
Oli Hale
ASMS
about 1 year ago
Abstract deadline for
#ASMS2025
is February 7. Submit your abstract to present your work in Baltimore. This is a final deadline, please plan accordingly. Abstract submission instructions & criteria:
www.asms.org/conferences/...
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reposted by
Oli Hale
PastelBio
about 1 year ago
imzML Writer: An Easy-to-Use Python Pipeline for Conversion of Continuously Acquired Raw Mass Spectrometry Imaging Files to imzML Format
chemrxiv.org/engage/...
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#proteomics
#prot-preprint
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A small side project and also my first article as corresponding author is now published.
#TeamMassSpec
pubs.acs.org/doi/10.1021/...
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Infrared Photoactivation Enables nano-DESI MS of Protein Complexes in Tissue on a Linear Ion Trap Mass Spectrometer
Native mass spectrometry analysis of proteins directly from tissues can be performed by using nanospray-desorption electrospray ionization (nano-DESI). Typically, supplementary collisional activation ...
https://pubs.acs.org/doi/10.1021/jasms.4c00377
about 1 year ago
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The last bit of peel 👀 Simultaneously the least and most important part of installation.
#TeamMassSpec
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about 1 year ago
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The moment I knew I’d been sussed in Cairns, Australia.
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over 1 year ago
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Commuting by train in the UK is hateful.
over 1 year ago
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There is no device I hate more than the leaf blower.
over 1 year ago
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It’s Paris-Roubaix eve. I love the Tour of Flanders but P-R has been special to me since the first time I watched. It’s just another level of punishing.
almost 2 years ago
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It’s been a busy week.
about 2 years ago
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you reached the end!!
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