Using live-cell microscopy, we uncovered distinct bacterial subpopulations that primarily use certain combinations of these nanomachines: • some prioritize motility & T3SS • others shift to T6SS for antagonism All tuned by the usual suspect: cyclic di-GMP.

We recently described how T3SS effectors reach the injectisome with a shuttle system. But what about their chaperones? With live microscopy, proximity labeling and functional assays, @keithpint.bsky.social showed the Yersinia chaperone SycH already hands over its cargo in the cytosol. bit.ly/47hq6rM