Sotirios Kampranis
@skampranis.bsky.social
📤 60
📥 66
📝 4
Professor of Biochemical Engineering, University of Copenhagen
Terpenoids were long thought to come only in multiples of 5 carbons. We show that bacteria break this rule, producing diverse 17-carbon terpenoids, and uncover their biosynthesis, enzyme evolution, and remarkable molecular architectures.
pubs.acs.org/doi/10.1021/...
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Biosynthesis of 17-Carbon Terpenoids in Bacteria
Terpenoids are the largest group of specialized metabolites with broad biological activities and applications. While terpenoid biosynthesis was long thought to generate only backbones with multiples of five carbons, the discovery of bacterial 16-carbon terpenoids challenged this view. Here, we expand our understanding of terpenoid biosynthesis by identifying and characterizing 17-carbon terpenoids. Through genome mining and functional analysis, we reveal a diverse array of structurally complex C17 terpenoids synthesized via a conserved pathway. Two methyltransferases successively modify farnesyl diphosphate into a C17 diphosphate precursor, which terpene synthases convert into distinct C17 scaffolds. Phylogenetic analysis shows that C17 terpene synthases evolved multiple times from C16-associated enzymes, while the latter can also generate C17 terpenoids. These findings establish C16 synthases as an evolutionary reservoir for C17 biosynthesis, broadening our understanding of terpenoid diversity and evolution, while paving the way for the biological, chemical, and biotechnological exploration of this new terpenoid class.
https://pubs.acs.org/doi/10.1021/jacs.5c15311
28 days ago
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reposted by
Sotirios Kampranis
Ryo Yokoyama
9 months ago
Elucidation of the final steps in Taxol biosynthesis and its biotechnological production
www.nature.com/articles/s44...
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Elucidation of the final steps in Taxol biosynthesis and its biotechnological production - Nature Synthesis
In Taxol biosynthesis, the steps leading up to the intermediate baccatin III are understood, however, the final steps remain elusive. Here elucidation of the final steps of Taxol biosynthesis enables ...
https://www.nature.com/articles/s44160-025-00800-z
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reposted by
Sotirios Kampranis
Nature Synthesis
9 months ago
Now online: News & Views on the Article by Sotirios Kampranis & co-workers
www.nature.com/articles/s44...
($) Toward a Taxol-producing microbial cell factory by Vincent Courdavault & Nicolas Papon
www.nature.com/articles/s44...
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Toward a Taxol-producing microbial cell factory - Nature Synthesis
Reconstruction of the late enzymatic steps of the yew Taxol biosynthetic pathway in yeast enables the bioproduction of Taxol.
https://www.nature.com/articles/s44160-025-00801-y?utm_source=bluesky&utm_medium=social&utm_campaign=natsynth
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Our work on Taxol biosynthesis is now out at
@natsynth.nature.com
. You can read it here:
www.nature.com/articles/s44...
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Elucidation of the final steps in Taxol biosynthesis and its biotechnological production - Nature Synthesis
In Taxol biosynthesis, the steps leading up to the intermediate baccatin III are understood, however, the final steps remain elusive. Here elucidation of the final steps of Taxol biosynthesis enables ...
https://www.nature.com/articles/s44160-025-00800-z
9 months ago
0
19
12
reposted by
Sotirios Kampranis
Nature Synthesis
9 months ago
Now online: Article by Feiyan Liang,
@skampranis.bsky.social
& co-workers
@novo-nordisk.bsky.social
Elucidation of the final steps in Taxol biosynthesis and its biotechnological production
www.nature.com/articles/s44...
($)
#ChemSky
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Elucidation of the final steps in Taxol biosynthesis and its biotechnological production - Nature Synthesis
In Taxol biosynthesis, the steps leading up to the intermediate baccatin III are understood, however, the final steps remain elusive. Here elucidation of the final steps of Taxol biosynthesis enables ...
https://www.nature.com/articles/s44160-025-00800-z?utm_source=bluesky&utm_medium=social&utm_campaign=natsynth
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reposted by
Sotirios Kampranis
10 months ago
Delighted to share the latest research results of our group on a new synthetic method for non-canonical terpene skeletons. 🥳🥳🥳
add a skeleton here at some point
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Excited to share our latest article! We develop a systematic method to biosynthesize isoprenoid analogs with additional carbons in their skeletons, opening new avenues for biotechnological applications. Thank you:
@novo-nordisk.bsky.social
Read more:
www.nature.com/articles/s41...
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Systematic biotechnological production of isoprenoid analogs with bespoke carbon skeletons - Nature Communications
Current efforts on isoprenoid analogs bioproduction is mainly based on adding functional groups to existing isoprenoid core scaffolds. Here, the authors develop a yeast cell-based biocatalytic method ...
https://www.nature.com/articles/s41467-025-57494-2
11 months ago
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7
reposted by
Sotirios Kampranis
ross
11 months ago
Systematic biotechnological production of isoprenoid analogs with bespoke carbon skeletons
www.nature.com/articles/s41...
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Systematic biotechnological production of isoprenoid analogs with bespoke carbon skeletons - Nature Communications
Current efforts on isoprenoid analogs bioproduction is mainly based on adding functional groups to existing isoprenoid core scaffolds. Here, the authors develop a yeast cell-based biocatalytic method ...
https://www.nature.com/articles/s41467-025-57494-2
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