Site-specific, multi-kilobase insertions and over-writes in mammalian cells, without DSBs, HDR, recombinases, integrases, transposases, or active cell cycling? Using fully or predominantly single-stranded DNA donors? 1/15

📰 Hot off the press from the Xue & Sontheimer labs 🧬: Prime assembly enables precise genomic insertions up to 11 kb in cells, providing a universal strategy to correct many pathogenic mutations within a gene. Read it @nature.com: buff.ly/dYn0NjS @UMassChan.bsky.social @eriksontheimer.bsky.social