Thank you to my amazing collaborators and the PhD student doing the work, Sam! Please have a read, it is a really interesting paper!

An elegant engineering strategy to perform step-wise truncations of the iron-sulfur cluster domains in semi-synthetic [FeFe] hydrogenases to investigate in detail the influence on the catalytic properties of the enzyme and the inhibitors binding!

I couldn't be happier to see this nice piece of work out! It is like my baby project, which I dedicated a lot of time and effort! lnkd.in/eKpyxrM5http...

Congratulations @rmlab.bsky.social ! pubs.acs.org/doi/10.1021/...

Vincent, Morra, and Rodríguez-Maciá demonstrate that truncating the electron transfer domain of [FeFe]-hydrogenase does *not* diminish their catalytic efficiency (via ACS Catalysis). doi.org/10.1021/acsc...

Our first foray into the P450 world. Thanks to @bellp450enzyme.bsky.social for sharpening our GC-MS skill set @jamesbirrell.bsky.social for the microwaves😃 and Lewis for persevering 🧪

1️⃣ 𝗠𝗜𝗕𝗧𝗣-𝗕𝗕𝗦𝗥𝗖 - 𝟮𝟳𝘁𝗵 𝗡𝗼𝘃𝗲𝗺𝗯𝗲𝗿 𝟮𝟬𝟮𝟱 𝗱𝗲𝗮𝗱𝗹𝗶𝗻𝗲: warwick.ac.uk/fac/cross_fa... 2️⃣ 𝗖𝗘𝗡𝗧𝗔-𝗡𝗘𝗥𝗖 - 𝟳𝘁𝗵 𝗝𝗮𝗻𝘂𝗮𝗿𝘆 𝟮𝟬𝟮𝟲 𝗱𝗲𝗮𝗱𝗹𝗶𝗻𝗲: centa.ac.uk/studentship/... Any questions or more information? Please email me, happy to help! #metalloenzymes #biohybridcatalysts #sustainablechemistry #greenchemistry

Are you excited about #metalloenzymes and #biohybridcatalysts? Are you interested in investigating new approaches for #sustainable and #greenchemistry? If so, I have 𝘁𝘄𝗼 𝗣𝗵𝗱 𝗽𝗼𝘀𝗶𝘁𝗶𝗼𝗻𝘀 to join my group in 📅 Sept 2026 under the schemes. 👇+Info👇

We have a 𝗣𝗼𝘀𝘁𝗱𝗼𝗰𝘁𝗼𝗿𝗮𝗹 𝗥𝗲𝘀𝗲𝗮𝗿𝗰𝗵 𝗔𝘀𝘀𝗼𝗰𝗶𝗮𝘁𝗲 (𝗣𝗗𝗥𝗔) position for a project in collaboration with my colleagues Phil Ash & James Pickering, via the AMBER Postdoctoral Programme funded by MSCActions. Come and join us at @uniofleicester! 👇+info👇

Information about the project here: le.ac.uk/research/ins... Information about the position and how to apply: jobs.le.ac.uk/vacancies/11... Deadline 7 Dec 2025! For any enquires please send an email!

Delightful combination of spectroscopies (electronic, microwave) and protein crystallography in @jarworrall.bsky.social's new paper on cytochrome P450 decarboxylase from Staphylococcus aureus 🤩 doi.org/10.1016/j.ji...

We are delighted to confirm that the and Other Redox Metalloenzymes will take place from 26-30 July 2026 at the University of Leicester, UK. Details of the conference, including regularly updated confirmed speakers, are available on our website (link in bio).

Congrats @rmlab.bsky.social and co-authors on "Two-dimensional infrared spectroscopy as a tool to reveal the vibrational and molecular structure of [FeFe] hydrogenases" in Chemical Science pubs.rsc.org/en/content/a...

Join us next Tuesday May 27 for a Desktop Seminar on the Chemistry of Inorganic Clusters! Featuring talks and live Q&A from Stephen Ragsdale (University of Michigan) and Patrick Holland (Yale University), this is one you don't want to miss! Register and find more details rsc.zoom.us/webinar/regi...

We probed both directions, and it really seems to be the F-domain that modulates the on/off dynamics. We didn't investigate DdH tho...

We have positions for pCNF SDM of CpI in a recent grant application... Very encouraging to see Kylie's data!

We believe that these protein-protein-interactions propell fast & repeated one-electron-transfer reactions, driving multi-electron redox catalysis with a one-electron mediator. In hydrogenase, and other oxidoreductases as well. Find all the details in the paper: pubs.rsc.org/en/content/a...

Uli's brilliant microscale thermophoresis experiments show that *only* reduced ferredoxin binds to hydrogenase, which facilitates assigning the secondary structural changes to redox changes and a shift from an "attractive" to a "repulsive" configuration.

Infrared difference spectra reveal that the reduction of ferredoxin is accompanied with larger secondary structural changes - clearly associated with the "F-domain" of [FeFe]-hydrogenase, as reference experiments with "minimal hydrogenase" CrHydA1 lack this spectral feature. A surprise‼️

Interestingly, in the ferredoxin from C. pasteurianum, reduction alone does *not* affect the nitrile frequency. Only in complex with [FeFe]-hydrogenase CpI, redox changes affect the artificial amino acid...

Ross @milton-group.bsky.social came up with a very similar idea: how about introducing cyanophenylalanine to a (bacterial) ferredoxin that binds two [4Fe-4S] clusters? One might learn where the electron resides as most ferredoxins are considered one-electron redox mediators... 🧵

"redox-dependent nitrile band shifts" 🤩

Lovely stuff Zehui et al.!

Applying this genetic approach to [FeFe]-hydrogenase, the nitrile band allows probing the FeS cluster redox states AND the H-cluster. This will be a powerful tool in other FeS proteins as well! doi.org/10.1002/cbic...

First off, let me highlight recent work from Vincent et al.: the authors modified plant ferredoxin with cyanophenylalanine and succeeded in measuring FeS-redox-dependent nitrile band shifts in the IR spectra.

Who's ready to talk about ferredoxin?

It is a real pleasure to collaborate with you guys! 😊

Very happy to see this out! Take a look at this interesting paper on the application of 2D IR to [FeFe] hydrogenases 👇

Out now in @chemicalscience.rsc.org: We apply 2DIR spectroscopy to study [FeFe] hydrogenase for the first time. Check it out! pubs.rsc.org/en/content/a...

As part of this work we explicitly measured the XFEL temporal pulse profiles with a Wakefield device rather than inferring pulse length from electron bunch parameters

Our new paper exploring the effect of XFEL pulse length and pulse intensity on electron density maps of two radiation sensitive proteins. A great collaboration between Diamond, SwissFEL and University of Essex's Worrall group. journals.iucr.org/m/issues/202...

The meanest title ☺️

Here's what my former employer @tuberlin.bsky.social wrote about our work (🇩🇪) www.tu.berlin/news/pressem...

Short press piece @unipotsdam.bsky.social about our new article @nature.com on methyl coenzyme M reductase (MCR) www.uni-potsdam.de/en/headlines...

From our Emerging Investigators collection 🎉 'Terminal hydride formation in [FeFe] hydrogenase: understanding the role of the dithiolate bridgehead' by Patricia Rodríguez-Maciá (@rmlab.bsky.social), James Birrell and colleagues. #OpenAccess 🔓

Have you read it yet? ☺️

#2 out of 3 bfu family bifurcating structure with our CryoEM collaborators. Cryo-EM Structures Define the Electron Bifurcating Flavobicluster and Ferredoxin Binding Site in an Archaeal Nfn-Bfu Transhydrogenase - Journal of Biological Chemistry www.jbc.org/article/S002...

Very interesting! Toward a deeper understanding of the dithiolate ligand of [FeFe]-hydrogenase.

Big title for a DFT-only research paper... onlinelibrary.wiley.com/doi/full/10....

Big day today! Excited to share our new publication in @chemcomm.rsc.org about the formation of the very important hydride state in [FeFe] hydrogenases! Enjoy the reading! ☺️ pubs.rsc.org/en/content/a...

In this Perspective, Patricia and colleagues detail the missing pieces that are still needed to understand the full catalytic cycle of these hydrogen conversion enzymes. Find out about how detailed insights have revealed a sophisticated, optimised, molecular catalyst, the active site H-cluster!

Patricia Rodríguez-Maciá holds an interest in [FeFe] hydrogenases and has published a number of works exploring the catalysis of these systems. Her latest Perspective on these enzymes is available to read here: doi.org/10.1039/D4SC... #ChemSky

Not so relevant for infrared spectroscopy, unfortunatelly, but for any other kind of SEC these guys got some hot designs, love it! redox.me/collections/...

Forgot to tag @jamesbirrell.bsky.social‼️

Interesting preprint from Plumere and Winkler et al. on [FeFe]-hydrogenase maturation (PDF) chemrxiv.org/engage/api-g...

In Nature Chemical Biology today, we reveal how microbes clean our atmospheres by consuming carbon monoxide (CO) gas. A methodological tour de force from Ashleigh Kropp, Rhys Grinter, and David Gillett with broad implications for the atmosphere and bioenergetics. www.nature.com/articles/s41...